Gladiolus hybridus Hort is one of the world＇s famous cutting flowers. It is very popular because of the big size, bright color, various shape, and long period of bloom. New species should be cultivated in order to me...Gladiolus hybridus Hort is one of the world＇s famous cutting flowers. It is very popular because of the big size, bright color, various shape, and long period of bloom. New species should be cultivated in order to meet the consumers＇ need of asking for the new. Among the technologies of cultivating new species of flowers, mutagenic breeding is a shortcut. This study treated corm bud of G. hybridus Hort with EMS of different consistency. Then M1 after treated was analyzed by RAPD. The result showed that EMS was a very effective mutagenic agent for the corm bud of G. hybridus Hort. With the increase of consistency, the mutagenic range increased first, then decreased, among which 0.6% EMS treatment had the biggest influence. However, with the same EMS consistency, there was not close relevancy between the amount of mutagenic agent and the divergence of plant＇s genomes, which offered a molecular basis for selecting plants with good mutation.展开更多
The purpose of this study is to find a new gene resource for the researches of molecular breeding of Rosaceae plants disease-resistance. Pyrus ussuriensis Maxim is used as a starting material to clone the full-length ...The purpose of this study is to find a new gene resource for the researches of molecular breeding of Rosaceae plants disease-resistance. Pyrus ussuriensis Maxim is used as a starting material to clone the full-length cDNA of NPR1（nonexpressor of pathogenesis- related genes 1） which is a key regulator in SA （salicylic acid）-mediated systemic acquired resistance （SAR） by homologous cloning and RACE techniques. The length of the cDNA sequence was 1 767 bp, the ORF was 1 761 bp, it coded 586 amino acids, pi=5.58, the relative molecular weight was 65.009 ku, contained 19 kinds of amino acids, and had full BTB/POZ and ANK domains. Compared the homology of NPR1 gene in GenBank database, the homology with Pyrus pyrifolia, Arabidopsis thaliana, Nicotiana tabacum, Lycopersicon esculentum, Oryza sativa, Helianthus annuus were 98%, 62%, 68%, 65%, 57%, 63%. The homology offunctional area were 99%, 78%, 82%, 79%, 74%, 77%. This NPR1 gene was considered as homologic gene of Pyrus ussuriensis Maxim and named PumNPR1.展开更多
ISSR analysis is applied in this study to research the classification and genetic relationship of 26 cultivars of G.hybridus Hort.Total 19 arbitrary primers screened from 100 primers were used for further PCR and dive...ISSR analysis is applied in this study to research the classification and genetic relationship of 26 cultivars of G.hybridus Hort.Total 19 arbitrary primers screened from 100 primers were used for further PCR and diversity analysis.A total of 110 ISSR sites were detected with a mean of 5.63 fragments amplified for each primer.A total of 103 polymorphic DNA fragments were detected among all the 110 amplified fragments,which accounted for a high level of 93.6%of all and could be used to identify different cultivars.The results revealed that the idioplasm resource of G.hybridus Hort cutting flower cultivar had a narrow genetic basis on molecular level,and certain genetic relationship existed in summer large-flowers of G.hybridus Hort varieties.展开更多
基金Supported by Postdoctoral Fund of China (20080430871)Postdoctoral Funds Project of Heilongjiang Province+1 种基金Innovation Talent Research Fund of Harbin City (2006RFLXN007)Education Project Fund of Heilongjiang Province (11511037)
文摘Gladiolus hybridus Hort is one of the world＇s famous cutting flowers. It is very popular because of the big size, bright color, various shape, and long period of bloom. New species should be cultivated in order to meet the consumers＇ need of asking for the new. Among the technologies of cultivating new species of flowers, mutagenic breeding is a shortcut. This study treated corm bud of G. hybridus Hort with EMS of different consistency. Then M1 after treated was analyzed by RAPD. The result showed that EMS was a very effective mutagenic agent for the corm bud of G. hybridus Hort. With the increase of consistency, the mutagenic range increased first, then decreased, among which 0.6% EMS treatment had the biggest influence. However, with the same EMS consistency, there was not close relevancy between the amount of mutagenic agent and the divergence of plant＇s genomes, which offered a molecular basis for selecting plants with good mutation.
文摘The purpose of this study is to find a new gene resource for the researches of molecular breeding of Rosaceae plants disease-resistance. Pyrus ussuriensis Maxim is used as a starting material to clone the full-length cDNA of NPR1（nonexpressor of pathogenesis- related genes 1） which is a key regulator in SA （salicylic acid）-mediated systemic acquired resistance （SAR） by homologous cloning and RACE techniques. The length of the cDNA sequence was 1 767 bp, the ORF was 1 761 bp, it coded 586 amino acids, pi=5.58, the relative molecular weight was 65.009 ku, contained 19 kinds of amino acids, and had full BTB/POZ and ANK domains. Compared the homology of NPR1 gene in GenBank database, the homology with Pyrus pyrifolia, Arabidopsis thaliana, Nicotiana tabacum, Lycopersicon esculentum, Oryza sativa, Helianthus annuus were 98%, 62%, 68%, 65%, 57%, 63%. The homology offunctional area were 99%, 78%, 82%, 79%, 74%, 77%. This NPR1 gene was considered as homologic gene of Pyrus ussuriensis Maxim and named PumNPR1.
基金Supported by Postdoctoral Fund of China(20080430871)Postdoctoral Funds Project of Heilongjiang Province(520-415037)Innovation Talent Research Fund of Harbin City(2006RFLXN007)
文摘ISSR analysis is applied in this study to research the classification and genetic relationship of 26 cultivars of G.hybridus Hort.Total 19 arbitrary primers screened from 100 primers were used for further PCR and diversity analysis.A total of 110 ISSR sites were detected with a mean of 5.63 fragments amplified for each primer.A total of 103 polymorphic DNA fragments were detected among all the 110 amplified fragments,which accounted for a high level of 93.6%of all and could be used to identify different cultivars.The results revealed that the idioplasm resource of G.hybridus Hort cutting flower cultivar had a narrow genetic basis on molecular level,and certain genetic relationship existed in summer large-flowers of G.hybridus Hort varieties.
