It is of great scientific significance to construct a 3D dynamic structural color with a special color effect based on the microlens array.However,the problems of imperfect mechanisms and poor color quality need to be...It is of great scientific significance to construct a 3D dynamic structural color with a special color effect based on the microlens array.However,the problems of imperfect mechanisms and poor color quality need to be solved.A method of 3D structural color turning on periodic metasurfaces fabricated by the microlens array and self-assembly technology was proposed in this study.In the experiment,Polydimethylsiloxane(PDMS)flexible film was used as a substrate,and SiO2 microspheres were scraped into grooves of the PDMS film to form 3D photonic crystal structures.By adjusting the number of blade-coated times and microsphere concentrations,high-saturation structural color micropatterns were obtained.These films were then matched with microlens arrays to produce dynamic graphics with iridescent effects.The results showed that by blade-coated two times and SiO2 microsphere concentrations of 50%are the best conditions.This method demonstrates the potential for being widely applied in the anticounterfeiting printing and ultra-high-resolution display.展开更多
目的大鼠海马神经元经过不同时间糖氧剥夺(oxygen and glucose deprivation,OGD)处理后,观察其线粒体形态改变与凋亡相关蛋白的变化。方法将原代培养大鼠海马神经元随机分为5组:空白对照组(对照组)与OGD处理30min组、45min组、60min组及...目的大鼠海马神经元经过不同时间糖氧剥夺(oxygen and glucose deprivation,OGD)处理后,观察其线粒体形态改变与凋亡相关蛋白的变化。方法将原代培养大鼠海马神经元随机分为5组:空白对照组(对照组)与OGD处理30min组、45min组、60min组及90min组,采用激光共聚焦显微镜观察线粒体形态;JC-1检测线粒体膜电位变化;Western blot检测胞质内磷酸化线粒体动力相关蛋白(P-Drp1蛋白)、细胞色素C(CytC)及含半胱氨酸的天冬氨酸水解蛋白3(caspase 3)蛋白的表达。结果随着OGD处理时间延长,(1)P-Drp1蛋白表达下降,线粒体裂解率逐渐增加(P<0.05);(2)线粒体膜电位下降越严重;(3)与对照组相比,60min组及90min组CytC、caspase 3蛋白的表达显著增加(P<0.05)。结论随着OGD处理延长,线粒体裂解增加,膜电位下降,膜通透性增加,且在OGD处理60min时伴随凋亡发生。展开更多
文摘It is of great scientific significance to construct a 3D dynamic structural color with a special color effect based on the microlens array.However,the problems of imperfect mechanisms and poor color quality need to be solved.A method of 3D structural color turning on periodic metasurfaces fabricated by the microlens array and self-assembly technology was proposed in this study.In the experiment,Polydimethylsiloxane(PDMS)flexible film was used as a substrate,and SiO2 microspheres were scraped into grooves of the PDMS film to form 3D photonic crystal structures.By adjusting the number of blade-coated times and microsphere concentrations,high-saturation structural color micropatterns were obtained.These films were then matched with microlens arrays to produce dynamic graphics with iridescent effects.The results showed that by blade-coated two times and SiO2 microsphere concentrations of 50%are the best conditions.This method demonstrates the potential for being widely applied in the anticounterfeiting printing and ultra-high-resolution display.
