该研究利用Illumina Mi Seq测序技术研究高温大曲发酵过程中真菌群落的演替规律。α多样性分析结果表明,高温大曲发酵过程中真菌群落的丰富度和多样性均呈先上升后下降的趋势,在第二次翻曲时,发现物种数(369.16)和辛普森指数(0.75)均达...该研究利用Illumina Mi Seq测序技术研究高温大曲发酵过程中真菌群落的演替规律。α多样性分析结果表明,高温大曲发酵过程中真菌群落的丰富度和多样性均呈先上升后下降的趋势,在第二次翻曲时,发现物种数(369.16)和辛普森指数(0.75)均达到最大,且极显著高于拆曲出库时(P<0.01);优势真菌属包括嗜热子囊菌属(Thermoascus)、嗜热真菌属(Thermomyces)、曲霉属(Aspergillus)、罗萨氏菌属(Rasamsonia)和丝衣霉菌属(Byssochlamys)。β多样性分析结果表明,高温大曲发酵过程中真菌群落结构发生了较大变化。线性判别分析效应值大小(LEfSe)分析结果表明,不同发酵阶段呈现出特征性优势菌,Phialosimplex、Thermoascus和Thermomyces可分别为第一次翻曲、第二次翻曲和拆曲出库阶段的生物标志物。相关性分析结果表明,真菌群落结构的变化可能与菌群间的互作关系密切相关。该研究结果为后续优化大曲发酵过程和提高产品品质奠定了基础。展开更多
Sediment particles,as one of the key components of drip irrigation technology,significantly affect the service life of emitters and restrict the popularization of drip irrigation technology.Hence,two types of patch dr...Sediment particles,as one of the key components of drip irrigation technology,significantly affect the service life of emitters and restrict the popularization of drip irrigation technology.Hence,two types of patch drip irrigation emitters,focusing on the anti-clogging performance through the experiment,were investigated.The dynamic variations in the clogging characteristics of emitters,specifically were subjected to statistical analysis.The movement mechanism of emitter clogging and discharging sediment was studied.The effects of emitter structure and position factors on emitter clogging were analyzed.The results show that the pressure-compensated emitter exhibits superior anti-clogging perfor-mance,with a service life that is 227.8%greater than that of the labyrinth channel emitter.A single structural factor cannot completely evaluate the anti-clogging performance of emitters.All factors causing emitter clogging should be considered comprehensively.Emitters contain sensitive sediment prone to clogging,however,significant blockage occurs primarily when the sediment content is elevated.The discharge of sediment,denoted as V90,from the emitter is affected by the accumulative effect of clogged sediment.These results may offer valuable insights for the application and advancement of drip irrigation technology.展开更多
文摘该研究利用Illumina Mi Seq测序技术研究高温大曲发酵过程中真菌群落的演替规律。α多样性分析结果表明,高温大曲发酵过程中真菌群落的丰富度和多样性均呈先上升后下降的趋势,在第二次翻曲时,发现物种数(369.16)和辛普森指数(0.75)均达到最大,且极显著高于拆曲出库时(P<0.01);优势真菌属包括嗜热子囊菌属(Thermoascus)、嗜热真菌属(Thermomyces)、曲霉属(Aspergillus)、罗萨氏菌属(Rasamsonia)和丝衣霉菌属(Byssochlamys)。β多样性分析结果表明,高温大曲发酵过程中真菌群落结构发生了较大变化。线性判别分析效应值大小(LEfSe)分析结果表明,不同发酵阶段呈现出特征性优势菌,Phialosimplex、Thermoascus和Thermomyces可分别为第一次翻曲、第二次翻曲和拆曲出库阶段的生物标志物。相关性分析结果表明,真菌群落结构的变化可能与菌群间的互作关系密切相关。该研究结果为后续优化大曲发酵过程和提高产品品质奠定了基础。
基金National Natural Science Foundation of China(52269011,52469008)。
文摘Sediment particles,as one of the key components of drip irrigation technology,significantly affect the service life of emitters and restrict the popularization of drip irrigation technology.Hence,two types of patch drip irrigation emitters,focusing on the anti-clogging performance through the experiment,were investigated.The dynamic variations in the clogging characteristics of emitters,specifically were subjected to statistical analysis.The movement mechanism of emitter clogging and discharging sediment was studied.The effects of emitter structure and position factors on emitter clogging were analyzed.The results show that the pressure-compensated emitter exhibits superior anti-clogging perfor-mance,with a service life that is 227.8%greater than that of the labyrinth channel emitter.A single structural factor cannot completely evaluate the anti-clogging performance of emitters.All factors causing emitter clogging should be considered comprehensively.Emitters contain sensitive sediment prone to clogging,however,significant blockage occurs primarily when the sediment content is elevated.The discharge of sediment,denoted as V90,from the emitter is affected by the accumulative effect of clogged sediment.These results may offer valuable insights for the application and advancement of drip irrigation technology.
