BACKGROUND:Opportunistic infection of Candida albicans(C.albicans) has become a serious problem in immunocompromised patients.The study aimed to explore the mechanism of enterogenous infection of C.albicans in immunoc...BACKGROUND:Opportunistic infection of Candida albicans(C.albicans) has become a serious problem in immunocompromised patients.The study aimed to explore the mechanism of enterogenous infection of C.albicans in immunocompromised rats under severe acute pancreatitis(SAP).METHODS:Sprague Dawley(SD) rats(n=100) were randomly assigned into 5 groups as the following:blank group,cyclophosphamide+ceftriaxone+SAP group,cyclophosphamide+ceftriaxone group,cyclophosphamide+SAP group,and cyclophosphamide group.The rats were sacrificed at 5and 10 days,and their jejunum,colon,mesenteric lymph nodes,pancreas,intestinal content,and blood were quickly collected to detect C.albicans.A region of the 25 S rRNA gene was chosen and amplified by polymerase chain reaction(PCR) to differentiate C.albicans genotypes.The amplified products were further sequenced and compared to judge their homology.RESULTS:Compared with the Cyclophosphamide group,the combination of immunosuppressants and broad-spectrum antibiotics significantly increased the colonization of C.albicans in intestine in 5 and 10 days.Pure SAP stress did not increase the opportunistic infection of C.albicans.The PCR products of C.albicans isolates all belonged to the genotype A family,and sequence alignment showed that the amplified fragments were homologous.CONCLUSION:The damage of immune system and broad-spectrum antimicrobial agents are important risk factors for opportunistic fungal infection.Intestinal tract is an important source for genotype-A C.albicans to translocate and invade into bloodstream.展开更多
Covering-based rough sets process data organized by a covering of the universe. A soft set is a parameterized family of subsets of the universe. Both theories can deal with the uncertainties of data. Soft sets have no...Covering-based rough sets process data organized by a covering of the universe. A soft set is a parameterized family of subsets of the universe. Both theories can deal with the uncertainties of data. Soft sets have not any restrictions on the approximate description of the object,and they might form a covering of the universe. From this viewpoint,we establish a connection between these two theories. Specifically,we propose a complementary parameter for this purpose. With this parameter,the soft covering approximation space is established and the two theories are bridged. Furthermore,we study some relations between the covering and the soft covering approximation space and obtain some significant results. Finally,we define a notion of combine parameter which can help us to simplify the set of parameters and reduce the storage requirement of a soft covering approximation space.展开更多
基金supported by grants from the Research Foundation of Shanghai Minhang District Municipal Commission of Health and Family Planning(2013MW12)the Research Foundation of Shanghai Municipal Commission of Health and Family Planning(201540136)
文摘BACKGROUND:Opportunistic infection of Candida albicans(C.albicans) has become a serious problem in immunocompromised patients.The study aimed to explore the mechanism of enterogenous infection of C.albicans in immunocompromised rats under severe acute pancreatitis(SAP).METHODS:Sprague Dawley(SD) rats(n=100) were randomly assigned into 5 groups as the following:blank group,cyclophosphamide+ceftriaxone+SAP group,cyclophosphamide+ceftriaxone group,cyclophosphamide+SAP group,and cyclophosphamide group.The rats were sacrificed at 5and 10 days,and their jejunum,colon,mesenteric lymph nodes,pancreas,intestinal content,and blood were quickly collected to detect C.albicans.A region of the 25 S rRNA gene was chosen and amplified by polymerase chain reaction(PCR) to differentiate C.albicans genotypes.The amplified products were further sequenced and compared to judge their homology.RESULTS:Compared with the Cyclophosphamide group,the combination of immunosuppressants and broad-spectrum antibiotics significantly increased the colonization of C.albicans in intestine in 5 and 10 days.Pure SAP stress did not increase the opportunistic infection of C.albicans.The PCR products of C.albicans isolates all belonged to the genotype A family,and sequence alignment showed that the amplified fragments were homologous.CONCLUSION:The damage of immune system and broad-spectrum antimicrobial agents are important risk factors for opportunistic fungal infection.Intestinal tract is an important source for genotype-A C.albicans to translocate and invade into bloodstream.
基金supported by National Natural Science Foundation of China under Grant No. 60873077/F020107the Science Research Project of Zhangzhou Normal University under Grant No. SK09002
文摘Covering-based rough sets process data organized by a covering of the universe. A soft set is a parameterized family of subsets of the universe. Both theories can deal with the uncertainties of data. Soft sets have not any restrictions on the approximate description of the object,and they might form a covering of the universe. From this viewpoint,we establish a connection between these two theories. Specifically,we propose a complementary parameter for this purpose. With this parameter,the soft covering approximation space is established and the two theories are bridged. Furthermore,we study some relations between the covering and the soft covering approximation space and obtain some significant results. Finally,we define a notion of combine parameter which can help us to simplify the set of parameters and reduce the storage requirement of a soft covering approximation space.
