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鸭骨骼肌TNNI1基因CpG岛区甲基化与mRNA差异表达
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作者 姬改革 束婧婷 +4 位作者 单艳菊 章明 屠云洁 巨晓军 刘一帆 《江苏农业学报》 CSCD 北大核心 2018年第6期1312-1318,共7页
本研究旨在通过克隆鸭慢速骨骼肌型肌钙蛋白I 1(Slow skeletal muscle troponin I 1,TNNI1)基因5'侧翼区序列,检测鸭骨骼肌组织TNNI1基因的mRNA表达水平和启动子CpG岛区甲基化状态,初步探索TNNI1基因转录调控机制。采用染色体步移... 本研究旨在通过克隆鸭慢速骨骼肌型肌钙蛋白I 1(Slow skeletal muscle troponin I 1,TNNI1)基因5'侧翼区序列,检测鸭骨骼肌组织TNNI1基因的mRNA表达水平和启动子CpG岛区甲基化状态,初步探索TNNI1基因转录调控机制。采用染色体步移方法克隆测序获得鸭TNNI1基因5'侧翼区序列,进行生物信息学分析,采用荧光定量PCR(Real-time quantitative PCR,RT-PCR)检测鸭胸肌和腿肌TNNI1基因mRNA表达水平,采用亚硫酸氢盐测序法(Bisulfite sequencing PCR,BSP)检测核心启动子区CpG岛在鸭肌肉组织中的甲基化水平。结果表明,克隆获得鸭TNNI1基因5'侧翼区序列2 078 bp,预测存在2个CpG岛,其中CpG岛(-2 032~-1 833 bp)位于预测的核心启动子区内,并存在多个真核生物结构元件和转录因子结合位点;甲基化检测发现,其总体甲基化水平在胸肌和腿肌组织中分别为52. 66%、57. 04%,差异不显著(P>0. 05);荧光定量检测结果表明,鸭胸肌和腿肌TNNI1基因表达量差异显著(P<0. 05);相关性分析结果表明,CpG4位点甲基化程度与胸肌TNNI1基因表达量呈极显著负相关(P<0. 01)。鸭胸肌和腿肌TNNI1基因的mRNA表达量存在显著差异,其总体甲基化水平无显著差异。在胸肌中,启动子区CpG4位点可能通过甲基化修饰影响TNNI1基因的转录调控。 展开更多
关键词 TNNI1基因 启动子 MRNA表达 甲基化
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Structure design and characteristics analysis of a cylindrical giant magnetostrictive actuator for ball screw preload 被引量:1
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作者 ju xiao-jun LIN Ming-xing +2 位作者 FAN Wen-tao BU Qing-qiang WU Xiao-jian 《Journal of Central South University》 SCIE EI CAS CSCD 2018年第7期1799-1812,共14页
In order to achieve automatic adjustment of the double-nut ball screw preload, a magnetostrictive ball screw preload system is proposed. A new cylindrical giant magnetostrictive actuator (CGMA), which is the core co... In order to achieve automatic adjustment of the double-nut ball screw preload, a magnetostrictive ball screw preload system is proposed. A new cylindrical giant magnetostrictive actuator (CGMA), which is the core component of the preload system, is developed using giant magnetostrictive material (GMM) with a hole. The pretightening force of the CGMA is determined by testing. And the magnetic circuit analysis method is introduced to calculate magnetic field intensity of the actuator with a ball screw shaft. To suppress the thermal effects on the magnetostrictive outputs, an oil cooling method which can directly cool the heat source is adopted. A CGMA test platform is established and the static and dynamic output characteristics are respectively studied. The experimental results indicate that the CGMA has good linearity and no double-frequency effect under the bias magnetic field and the output accuracy of the CGMA is significantly improved with cooling measures. Although the output decreased with screw shaft through the actuator, the performance of CGMA meets the design requirements for ball screw preload with output displacement more than 26 μm and force up to 6200 N. The development of a CGMA will provide a new approach for automatic adjustment of double-nut ball screw preload. 展开更多
关键词 ball screw preload cylindrical giant magnetostrictive actuator (CGMA) structure design output characteristics
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