By detecting the influence of six main ingredients of PM2.5 mineral dusts on the A549 cell morphology, proliferation inhibition rate, micronuclei and DNA damage, to explore the genotoxicity of PM2.5 mineral dusts. (1)...By detecting the influence of six main ingredients of PM2.5 mineral dusts on the A549 cell morphology, proliferation inhibition rate, micronuclei and DNA damage, to explore the genotoxicity of PM2.5 mineral dusts. (1) After exposure to six kinds of dusts of 200 μg/mL concentration for 24 hours, the morphology of A549 cells were observed using Wright-Giemsa staining. (2) After exposure to different concentrations of mineral dusts for 24 hours, the proliferation inhibition rate of A549 cells was detected by MTT assay. (3) Cells were exposed to PM2.5 mineral dusts at a concentration of 200 μg/mL for 24 h. After Wright-Giemsa staining, the rates of micronucleus cells were counted under oil microscope. (4) Observe Comet phenomenon by SCGE electrophoresis, the degree of DNA damage was observed by OTM. (1) Compared to the control group, membrane destruction, nuclear pyknosis and mineral surface adhesion were mainly seen in the Sericite group and Albite group. In the Quartz group and Montmorillonite group, enlarged cell gaps, loosely arranged cells, absorption of a large number of minerals on the cell surface, and cell pyknosis were observed. (2) The proliferation inhibition rate of the six kinds of dusts to A549 cells were (from large to small): KWC-M>Nano-SiO2>KWC-S>KWC-Q>KWC-A>KWC-C.The dust concentration was positively related to the inhibition of cell proliferation rate. (3) With the dusts concentration increased, the incidence of micronuclei gradually increased. The rate was positively correlated to exposure concentration. (4) The six mineral dusts can damage DNA of the A549 cells by dose-response relationship.The higher concentration of the mineral dusts, the more obvious of the DNA damagenation. There’s statistically significant compared with the control group. The six main ingredients of the PM2.5 mineral dusts can change A549 cell morphology from varying degrees, improve proliferation inhibition rate of the cells, increase the number of micronuclei cells, damage DNA.Then we come to the conclusion that PM2.5 mineral dusts can change the genotoxicity of the cells.展开更多
基金Project supported by the State Key Program of National Natural Science of China(No.41130746)
文摘By detecting the influence of six main ingredients of PM2.5 mineral dusts on the A549 cell morphology, proliferation inhibition rate, micronuclei and DNA damage, to explore the genotoxicity of PM2.5 mineral dusts. (1) After exposure to six kinds of dusts of 200 μg/mL concentration for 24 hours, the morphology of A549 cells were observed using Wright-Giemsa staining. (2) After exposure to different concentrations of mineral dusts for 24 hours, the proliferation inhibition rate of A549 cells was detected by MTT assay. (3) Cells were exposed to PM2.5 mineral dusts at a concentration of 200 μg/mL for 24 h. After Wright-Giemsa staining, the rates of micronucleus cells were counted under oil microscope. (4) Observe Comet phenomenon by SCGE electrophoresis, the degree of DNA damage was observed by OTM. (1) Compared to the control group, membrane destruction, nuclear pyknosis and mineral surface adhesion were mainly seen in the Sericite group and Albite group. In the Quartz group and Montmorillonite group, enlarged cell gaps, loosely arranged cells, absorption of a large number of minerals on the cell surface, and cell pyknosis were observed. (2) The proliferation inhibition rate of the six kinds of dusts to A549 cells were (from large to small): KWC-M>Nano-SiO2>KWC-S>KWC-Q>KWC-A>KWC-C.The dust concentration was positively related to the inhibition of cell proliferation rate. (3) With the dusts concentration increased, the incidence of micronuclei gradually increased. The rate was positively correlated to exposure concentration. (4) The six mineral dusts can damage DNA of the A549 cells by dose-response relationship.The higher concentration of the mineral dusts, the more obvious of the DNA damagenation. There’s statistically significant compared with the control group. The six main ingredients of the PM2.5 mineral dusts can change A549 cell morphology from varying degrees, improve proliferation inhibition rate of the cells, increase the number of micronuclei cells, damage DNA.Then we come to the conclusion that PM2.5 mineral dusts can change the genotoxicity of the cells.
