The study aimed to optimize the induction and differentiation medium by exploreing different tissue culture of Saposhnikovia divaricata （Turcz.） Schischk. In tissue culture with the root, stem segments, young leaf, ...The study aimed to optimize the induction and differentiation medium by exploreing different tissue culture of Saposhnikovia divaricata （Turcz.） Schischk. In tissue culture with the root, stem segments, young leaf, cotyledonary node and axillary bud of Saposhnikovia divaricata （Turcz.） Schischk as explants, a lot of plantleles were obtained and the corresponding plant regeneration-system was established. The results showed that when use MS＋1.0 mg·L^-1 6-BA＋0.2 mg·L^-1 NAA as callus induction medium, the cotyledonary node had the highest bourgeon rate, and its callus was better than any others; MS＋2 mg·L^-1 6-BA＋0.4 mg·L^-1 NAA was the best adventitious buds induction medium, and the best adventitious buds induced condition was 3% sucrose as carbon source, illumination for 12-14 h·d^-1 and pH 5.8, The best rootage medium was 1/2 MS＋0.5 mg·L^-1 NAA.展开更多
Now every laboratory is facing a common problem that is seriously lacking in the standard reference molecule in the GMC detecting research center. Constructing the recombinant plasmid is the urgent demand. In order to...Now every laboratory is facing a common problem that is seriously lacking in the standard reference molecule in the GMC detecting research center. Constructing the recombinant plasmid is the urgent demand. In order to overcome this problem, this study respectively cloned CrylA（B）, BAR, CP4-EPSPS, PAT and RBCL genes into PMD 18-T vector. This constructed plasmid could be used as the standard reference molecule in the qualitative detecting of the exogenous CP4-EPSPS gene, CrylA（B） gene, BAR gene and PAT gene. Identification by double restriction endonuclease digestion and identification by PCR proved that the test result was positive. All of these indicated that the established reference molecules in this study were suitable for the qualitative analysis standard of this GMC detection.展开更多
基金Supported by Natural Science Foundation of Heilongjiang Province (C2005-31)
文摘The study aimed to optimize the induction and differentiation medium by exploreing different tissue culture of Saposhnikovia divaricata （Turcz.） Schischk. In tissue culture with the root, stem segments, young leaf, cotyledonary node and axillary bud of Saposhnikovia divaricata （Turcz.） Schischk as explants, a lot of plantleles were obtained and the corresponding plant regeneration-system was established. The results showed that when use MS＋1.0 mg·L^-1 6-BA＋0.2 mg·L^-1 NAA as callus induction medium, the cotyledonary node had the highest bourgeon rate, and its callus was better than any others; MS＋2 mg·L^-1 6-BA＋0.4 mg·L^-1 NAA was the best adventitious buds induction medium, and the best adventitious buds induced condition was 3% sucrose as carbon source, illumination for 12-14 h·d^-1 and pH 5.8, The best rootage medium was 1/2 MS＋0.5 mg·L^-1 NAA.
基金Supported by the Innovative Team Funds of Northeast Agricultural University (CXT004-3-2)Foundation of Heilongjiang Educational Committee (11511030)
文摘Now every laboratory is facing a common problem that is seriously lacking in the standard reference molecule in the GMC detecting research center. Constructing the recombinant plasmid is the urgent demand. In order to overcome this problem, this study respectively cloned CrylA（B）, BAR, CP4-EPSPS, PAT and RBCL genes into PMD 18-T vector. This constructed plasmid could be used as the standard reference molecule in the qualitative detecting of the exogenous CP4-EPSPS gene, CrylA（B） gene, BAR gene and PAT gene. Identification by double restriction endonuclease digestion and identification by PCR proved that the test result was positive. All of these indicated that the established reference molecules in this study were suitable for the qualitative analysis standard of this GMC detection.
