OBJECTIVE To investigate effects of Wudang cherry on urate excretion and renal function and examined whether renal organic ion transporters were involved in potassium oxonateinduced hyperuricemic mice.METHODS The mode...OBJECTIVE To investigate effects of Wudang cherry on urate excretion and renal function and examined whether renal organic ion transporters were involved in potassium oxonateinduced hyperuricemic mice.METHODS The model of hyperuricemic mice was induced by intraperitoneal injection of potassium oxonate(250 mg·kg^(-1))for 7 d.Water extracts of Wudang cherry at 500 mg·kg^(-1)were orally administered to hyperuricemic mice for 7 d,benzbromarone(20 mg·kg^(-1))and allopurinol(20 mg·kg^(-1))were given as positive controls,vehicle control group was given equal normal saline.Serum and urine levels of uric acid were measured in hyperuricemic and normal mice.Simultaneously,the m RNA and protein levels of mouse urate transporter 1(m URAT1),glucose transporter 9(mGLUT9),organic anion transporters(mOAT1 and mOAT3),ATP-binding cassette,subfamily G,membrane 2(mABCG2)and organic cation/carnitine transporters(m OCT1,m OCT2,m OCTN1 and m OCTN2)in the kidney were analyzed by Western blot,RT-PCR,immunohistochemical and immunofluorescent assay,respectively.RESULTS Wudang cherry significantly reduced serum uric acid levels and increased urine uric acid levels in hyperuricemic mice.And it effectively reversed potassium oxonate-induced alterations in renal m URAT1,mGLUT9,mOAT1,mOAT3 and mABCG2 m RNA and protein levels,resulting in the enhancement of renal urate excretion in mice.Moreover,Wudang Cherry increased renal m OCT1,m OCT2,m OCTN1 and m OCTN2 m RNA and protein levels,and improved renal impairment in this model.CONCLUSION Wudang cherry processes uricosuric and nephroprotective actions by regulating renal organic ion transporters in hyperuricemic mice.展开更多
The pyrolysis of cyclohexane,methylcyclohexane,and ethylcyclohexane have been studied behind reflected shock waves at pressures of 5 and10 bar and at temperatures of 930-1550 K for 0.05%fuel diluted by Argon.A single-...The pyrolysis of cyclohexane,methylcyclohexane,and ethylcyclohexane have been studied behind reflected shock waves at pressures of 5 and10 bar and at temperatures of 930-1550 K for 0.05%fuel diluted by Argon.A single-pulse shock tube(SPST)is used to perform the pyrolysis experiments at reaction times varying from 1.65 to 1.74 ms.Major products are obtained and quantified using gas chromatography analysis.A flame ionization detector and a thermal conductivity detector are used for species identification and quantification.Kinetic modeling has been performed using several detailed and lumped chemical kinetic mechanisms.Differences in modeling results among the kinetic models are described.Reaction path analysis and sensitivity analysis are performed to determine the important reactions controlling fuel pyrolysis and their influence on the predicted concentrations of reactant and product species profiles.The present work provides new fundamental knowledge in understating pyrolysis characteristics of cyclohexane compounds and additional data set for detailed kinetic mechanism development.展开更多
目的探讨无创头架结合改良穿刺探针在高血压性基底节区脑出血手术中的应用价值。方法选取2017年1月至2020年12月复旦大学附属金山医院符合纳入标准和排除标准的高血压性基底节区脑出血患者76例,随机分成无创头架组和有创头架组,进行神...目的探讨无创头架结合改良穿刺探针在高血压性基底节区脑出血手术中的应用价值。方法选取2017年1月至2020年12月复旦大学附属金山医院符合纳入标准和排除标准的高血压性基底节区脑出血患者76例,随机分成无创头架组和有创头架组,进行神经导航辅助脑内血肿微创穿刺置管引流。其中,无创头架组使用改良穿刺探针。比较两组患者手术时间、导航注册误差、住院周期、肺部感染率及术后6个月神经功能恢复情况。结果两组患者在年龄、性别、入院收缩压、入院时血肿量和入院格拉斯哥昏迷评分(Glasgow Coma Scale,GCS)等基线数据差异无统计学意义,术后6个月神经功能恢复亦无统计学差异。然而,在手术时长、导航注册误差、住院时长、肺部感染率等方面无创头架组均优于有创头架组,差异有显著统计学意义(P<0.01)。结论无创头架结合改良穿刺探针在神经导航辅助脑内血肿微创穿刺置管治疗高血压性基底节区脑出血手术中具有注册误差小、手术时间短、操作简单精准、住院周期短、并发症少等优势。展开更多
基金supported by National Natural Science Foundation of China(81402994)Natural Science Foundation of Hubei Province(2014CFB652)"2011 Plan" Open Fund Innovation Team Project of Hubei Province(2011JH-2014CXTT08)
文摘OBJECTIVE To investigate effects of Wudang cherry on urate excretion and renal function and examined whether renal organic ion transporters were involved in potassium oxonateinduced hyperuricemic mice.METHODS The model of hyperuricemic mice was induced by intraperitoneal injection of potassium oxonate(250 mg·kg^(-1))for 7 d.Water extracts of Wudang cherry at 500 mg·kg^(-1)were orally administered to hyperuricemic mice for 7 d,benzbromarone(20 mg·kg^(-1))and allopurinol(20 mg·kg^(-1))were given as positive controls,vehicle control group was given equal normal saline.Serum and urine levels of uric acid were measured in hyperuricemic and normal mice.Simultaneously,the m RNA and protein levels of mouse urate transporter 1(m URAT1),glucose transporter 9(mGLUT9),organic anion transporters(mOAT1 and mOAT3),ATP-binding cassette,subfamily G,membrane 2(mABCG2)and organic cation/carnitine transporters(m OCT1,m OCT2,m OCTN1 and m OCTN2)in the kidney were analyzed by Western blot,RT-PCR,immunohistochemical and immunofluorescent assay,respectively.RESULTS Wudang cherry significantly reduced serum uric acid levels and increased urine uric acid levels in hyperuricemic mice.And it effectively reversed potassium oxonate-induced alterations in renal m URAT1,mGLUT9,mOAT1,mOAT3 and mABCG2 m RNA and protein levels,resulting in the enhancement of renal urate excretion in mice.Moreover,Wudang Cherry increased renal m OCT1,m OCT2,m OCTN1 and m OCTN2 m RNA and protein levels,and improved renal impairment in this model.CONCLUSION Wudang cherry processes uricosuric and nephroprotective actions by regulating renal organic ion transporters in hyperuricemic mice.
文摘The pyrolysis of cyclohexane,methylcyclohexane,and ethylcyclohexane have been studied behind reflected shock waves at pressures of 5 and10 bar and at temperatures of 930-1550 K for 0.05%fuel diluted by Argon.A single-pulse shock tube(SPST)is used to perform the pyrolysis experiments at reaction times varying from 1.65 to 1.74 ms.Major products are obtained and quantified using gas chromatography analysis.A flame ionization detector and a thermal conductivity detector are used for species identification and quantification.Kinetic modeling has been performed using several detailed and lumped chemical kinetic mechanisms.Differences in modeling results among the kinetic models are described.Reaction path analysis and sensitivity analysis are performed to determine the important reactions controlling fuel pyrolysis and their influence on the predicted concentrations of reactant and product species profiles.The present work provides new fundamental knowledge in understating pyrolysis characteristics of cyclohexane compounds and additional data set for detailed kinetic mechanism development.
文摘目的探讨无创头架结合改良穿刺探针在高血压性基底节区脑出血手术中的应用价值。方法选取2017年1月至2020年12月复旦大学附属金山医院符合纳入标准和排除标准的高血压性基底节区脑出血患者76例,随机分成无创头架组和有创头架组,进行神经导航辅助脑内血肿微创穿刺置管引流。其中,无创头架组使用改良穿刺探针。比较两组患者手术时间、导航注册误差、住院周期、肺部感染率及术后6个月神经功能恢复情况。结果两组患者在年龄、性别、入院收缩压、入院时血肿量和入院格拉斯哥昏迷评分(Glasgow Coma Scale,GCS)等基线数据差异无统计学意义,术后6个月神经功能恢复亦无统计学差异。然而,在手术时长、导航注册误差、住院时长、肺部感染率等方面无创头架组均优于有创头架组,差异有显著统计学意义(P<0.01)。结论无创头架结合改良穿刺探针在神经导航辅助脑内血肿微创穿刺置管治疗高血压性基底节区脑出血手术中具有注册误差小、手术时间短、操作简单精准、住院周期短、并发症少等优势。
