We irradiated pea seeds with neutrons from a ^(252)Cf source and studied the radiation dose effects on various morphological development parameters during the growth of M_(1) generation peas.We found that in the dose ...We irradiated pea seeds with neutrons from a ^(252)Cf source and studied the radiation dose effects on various morphological development parameters during the growth of M_(1) generation peas.We found that in the dose range of 0.51-9.27 Gy,with the increase in neutron-absorbed dose,the morphological development parameters of M_(1) generation peas at the initial seedling stage showed an obvious trend with three fluctuations.With the development of pea,this trend gradually weakened.Further analysis and verification showed that the main trend in the M_(1) generation of pea seeds was an inhibitory effect induced by neutron irradiation and there was a good linear correlation between the inhibitory effect and neutron absorption dose We successfully demonstrated the background removal of mutant plants and defined morphological developmen parameters for peas that match the overall development of plants.Our results will positively impact neutron mutation breeding and automatic agriculture.展开更多
Irradiation breeding is an important technique in the effort to solve food shortages and improve the quality of agricultural products.In this study,a field test was implemented on the M3 generation of two mutant pea p...Irradiation breeding is an important technique in the effort to solve food shortages and improve the quality of agricultural products.In this study,a field test was implemented on the M3 generation of two mutant pea plants gained from previous neutron radiation of pea seeds.The relationship between agronomic characteristics and yields of the mutants was investigated.Moreover,differences in physiological and biochemical properties and seed nutrients were analyzed.The results demonstrated that the plant height,effective pods per plant,and yield per plant of mutant Leaf-M1 were 45.0%,43.2%,and 50.9%higher than those of the control group,respectively.Further analysis attributed the increase in yield per plant to the increased branching number.The yield per plant of mutant Leaf-M2 was 7.8%higher than that of the control group,which could be related to the increased chlorophyll content in the leaves.There was a significant difference between the two mutants in the increase in yield per plant owing to morphological variation between the two mutants.There were significant differences in SOD activity and MDA content between the two mutants and the control,indicating that the physiological regulation of the two mutants also changed.In addition,the iron element content of seeds of the two mutants was about 10.9%lower than in the seeds of the control group,a significant difference.These findings indicate that the mutants Leaf-M1 and Leaf-M2 have breeding value and material value for molecular biological studies.展开更多
基金supported by the National Natural Science Foundation of China(Nos.11675069 and 12075106)the Natural Science Foundation of Gansu Province(No.20JR10RA607)the Fundamental Research Funds for the Central Universities of China(No.lzujbky-2020-kb09)。
文摘We irradiated pea seeds with neutrons from a ^(252)Cf source and studied the radiation dose effects on various morphological development parameters during the growth of M_(1) generation peas.We found that in the dose range of 0.51-9.27 Gy,with the increase in neutron-absorbed dose,the morphological development parameters of M_(1) generation peas at the initial seedling stage showed an obvious trend with three fluctuations.With the development of pea,this trend gradually weakened.Further analysis and verification showed that the main trend in the M_(1) generation of pea seeds was an inhibitory effect induced by neutron irradiation and there was a good linear correlation between the inhibitory effect and neutron absorption dose We successfully demonstrated the background removal of mutant plants and defined morphological developmen parameters for peas that match the overall development of plants.Our results will positively impact neutron mutation breeding and automatic agriculture.
基金supported by the National Natural Science Foundation of China(No.11675069)the Fundamental Research Funds for the Central Universities of China(No.lzujbky-2019-kb09)。
文摘Irradiation breeding is an important technique in the effort to solve food shortages and improve the quality of agricultural products.In this study,a field test was implemented on the M3 generation of two mutant pea plants gained from previous neutron radiation of pea seeds.The relationship between agronomic characteristics and yields of the mutants was investigated.Moreover,differences in physiological and biochemical properties and seed nutrients were analyzed.The results demonstrated that the plant height,effective pods per plant,and yield per plant of mutant Leaf-M1 were 45.0%,43.2%,and 50.9%higher than those of the control group,respectively.Further analysis attributed the increase in yield per plant to the increased branching number.The yield per plant of mutant Leaf-M2 was 7.8%higher than that of the control group,which could be related to the increased chlorophyll content in the leaves.There was a significant difference between the two mutants in the increase in yield per plant owing to morphological variation between the two mutants.There were significant differences in SOD activity and MDA content between the two mutants and the control,indicating that the physiological regulation of the two mutants also changed.In addition,the iron element content of seeds of the two mutants was about 10.9%lower than in the seeds of the control group,a significant difference.These findings indicate that the mutants Leaf-M1 and Leaf-M2 have breeding value and material value for molecular biological studies.
