枯草芽胞杆菌BAB−1是一株对设施蔬菜气传病害具有较好防治效果的生防菌,前期研究表明脂肽类抗生素是其产生的主要抑菌物质之一。为提高菌株BAB−1脂肽类抗菌物质的产量,本研究通过快速蛋白液相层析(Fast protein liquid chromatography,F...枯草芽胞杆菌BAB−1是一株对设施蔬菜气传病害具有较好防治效果的生防菌,前期研究表明脂肽类抗生素是其产生的主要抑菌物质之一。为提高菌株BAB−1脂肽类抗菌物质的产量,本研究通过快速蛋白液相层析(Fast protein liquid chromatography,FPLC)分析了5种碳源及5种氮源对菌株BAB−1脂肽类抗菌物质产量的影响,进一步采用溶血圈试验及排油圈法测定粗脂肽的性质。结果表明,熊果苷是BAB−1菌株产生surfactin的最佳碳源,其surfactin产量分别是葡萄糖、D−木糖、D−核糖及L−阿拉伯糖的2.95、7.01、4.26及5.60倍,此时菌株BAB−1粗脂肽的溶血活性与排油能力最强;熊果苷与葡萄糖利于该菌株产生fengycin,二者的fengycin产量没有显著差异,但显著高于D−木糖、D−核糖及L−阿拉伯糖。L−天冬酰胺与L−谷氨酸钠是菌株BAB−1产生抗菌脂肽的良好氮源,二者的surfactin与fengycin产量没有显著差异,但显著高于L−半胱氨酸、L−脯氨酸及L−谷氨酰胺,以这2种物质为氮源时菌株BAB−1粗脂肽的溶血活性与排油能力均较强。通过RT−qPCR技术分析了不同碳源对surfactin合成酶基因srfAA表达的影响结果表明,以熊果苷为碳源时,菌株BAB−1中srfAA基因的表达量相比葡萄糖提高了2.9倍。上述结果为菌株BAB−1产抗菌脂肽类物质发酵工艺的优化及其工业化生产提供了一定的理论支持。展开更多
WTA （weapon-target allocation） of air defense operation is a very complicated problem and current models focus on static and restricted WTA problem mostly. Based on the dynamic characteristics of air defense operati...WTA （weapon-target allocation） of air defense operation is a very complicated problem and current models focus on static and restricted WTA problem mostly. Based on the dynamic characteristics of air defense operational command and decision of warships＇ formation, a dynamic WTA model is established. Simulation results show that switch fire and repetition fire of anti-air weapon system affect the result of the air defense operation remarkably and the dynamic model is more satisfying than static ones. Related results are gained based on the analysis of the simulation results and the results are accordant with the intuitionistic tactical judgment. The model is some reference for the research of air defense C^3I system of warships＇ formation.展开更多
文摘枯草芽胞杆菌BAB−1是一株对设施蔬菜气传病害具有较好防治效果的生防菌,前期研究表明脂肽类抗生素是其产生的主要抑菌物质之一。为提高菌株BAB−1脂肽类抗菌物质的产量,本研究通过快速蛋白液相层析(Fast protein liquid chromatography,FPLC)分析了5种碳源及5种氮源对菌株BAB−1脂肽类抗菌物质产量的影响,进一步采用溶血圈试验及排油圈法测定粗脂肽的性质。结果表明,熊果苷是BAB−1菌株产生surfactin的最佳碳源,其surfactin产量分别是葡萄糖、D−木糖、D−核糖及L−阿拉伯糖的2.95、7.01、4.26及5.60倍,此时菌株BAB−1粗脂肽的溶血活性与排油能力最强;熊果苷与葡萄糖利于该菌株产生fengycin,二者的fengycin产量没有显著差异,但显著高于D−木糖、D−核糖及L−阿拉伯糖。L−天冬酰胺与L−谷氨酸钠是菌株BAB−1产生抗菌脂肽的良好氮源,二者的surfactin与fengycin产量没有显著差异,但显著高于L−半胱氨酸、L−脯氨酸及L−谷氨酰胺,以这2种物质为氮源时菌株BAB−1粗脂肽的溶血活性与排油能力均较强。通过RT−qPCR技术分析了不同碳源对surfactin合成酶基因srfAA表达的影响结果表明,以熊果苷为碳源时,菌株BAB−1中srfAA基因的表达量相比葡萄糖提高了2.9倍。上述结果为菌株BAB−1产抗菌脂肽类物质发酵工艺的优化及其工业化生产提供了一定的理论支持。
文摘WTA （weapon-target allocation） of air defense operation is a very complicated problem and current models focus on static and restricted WTA problem mostly. Based on the dynamic characteristics of air defense operational command and decision of warships＇ formation, a dynamic WTA model is established. Simulation results show that switch fire and repetition fire of anti-air weapon system affect the result of the air defense operation remarkably and the dynamic model is more satisfying than static ones. Related results are gained based on the analysis of the simulation results and the results are accordant with the intuitionistic tactical judgment. The model is some reference for the research of air defense C^3I system of warships＇ formation.
