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高效柴油降解菌的筛选及其对烷烃组分的降解
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作者 陈红初 张婷娣 +2 位作者 付玉丰 茹金涛 秦传玉 《中国环境科学》 EI CAS CSCD 北大核心 2024年第10期5723-5732,共10页
从东北某油田开采区油污土壤中分离筛选出一株柴油降解菌13-3,经形态观察、16S rRNA鉴定其属于不动杆菌属(Acinetobacter sp.).该菌株在20℃、180r/min的条件下培养7d,可将1g/L的柴油降解88.50%,与其他菌株相比,表现出优秀的柴油降解能... 从东北某油田开采区油污土壤中分离筛选出一株柴油降解菌13-3,经形态观察、16S rRNA鉴定其属于不动杆菌属(Acinetobacter sp.).该菌株在20℃、180r/min的条件下培养7d,可将1g/L的柴油降解88.50%,与其他菌株相比,表现出优秀的柴油降解能力;接种至10g/kg柴油污染土壤中,在20℃、土壤含水量为30%的条件下修复21d,柴油降解率可达71.7%,在柴油污染土壤修复应用中具有较大潜力.根据烷烃底物降解实验,菌株13-3的烷烃利用谱较广,对C_(14)~C_(22)的中长链烷烃均有降解,C_(17)~C_(20)的降解率可达90%以上.环境因素影响实验表明,菌株13-3可在pH值6~9、温度10~30℃、盐度(NaCl)0.5%~3%的范围内生长,在10g/L柴油浓度下仍可获得较好生长,具有良好的环境适应性,有望在复杂的环境条件下发挥其降解性能.通过全基因组测序分析,在菌株13-3的基因组中找到3个烷烃降解关键基因(alkB、almA、ladA),推测其降解烷烃途径为末端氧化. 展开更多
关键词 菌株筛选鉴定 不动杆菌 柴油降解 烷烃降解
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Resolving double-sided inverse heat conduction problem using calibration integral equation method
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作者 chen hong-chu 《Journal of Central South University》 SCIE EI CAS CSCD 2019年第8期2100-2108,共9页
In this paper, a novel calibration integral equation is derived for resolving double-sided, two-probe inverse heat conduction problem of surface heat flux estimation. In contrast to the conventional inverse heat condu... In this paper, a novel calibration integral equation is derived for resolving double-sided, two-probe inverse heat conduction problem of surface heat flux estimation. In contrast to the conventional inverse heat conduction techniques, this calibration approach does not require explicit input of the probe locations, thermophysical properties of the host material and temperature sensor parameters related to thermal contact resistance, sensor capacitance and conductive lead losses. All those parameters and properties are inherently contained in the calibration framework in terms of Volterra integral equation of the first kind. The Laplace transform technique is applied and the frequency domain manipulations of the heat equation are performed for deriving the calibration integral equation. Due to the ill-posed nature, regularization is required for the inverse heat conduction problem, a future-time method or singular value decomposition (SVD) can be used for stabilizing the ill-posed Volterra integral equation of the first kind. 展开更多
关键词 inverse heat conduction problem surface heat flux estimation calibration integral equation method
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