Immunoreactive substance P(Ir-SP)level,and substance P likeimmunoreactivity(SP-Li)in the spinal cord were observed with radioimmunoassay andimmunohistochemistry in rats after they were given an intraperitoneal injecti...Immunoreactive substance P(Ir-SP)level,and substance P likeimmunoreactivity(SP-Li)in the spinal cord were observed with radioimmunoassay andimmunohistochemistry in rats after they were given an intraperitoneal injection of mor-phine(7.5mg/kg)or electroacupunctured(3V and 3Hz)on the “Jiaji point”.It wasfound that the pain threshold(PT),Ir-SP level and SP-Li in the dorsal horn of the spi-nal cord were more significantly increased in the animals after the administration ofmorphine or electroacupuncture than in the control(P【0.05～0.01).The combined effectsof morphine and electropacupuncture were even more powerful than either of the agentswas administered singly.Naloxone could block the analgesic effect and the elevation ofIr-SP due to morphine or electroacupuncture.The findings suggest that there is a synergismbe tween morphine and electroacupuncture and the analgesic effect of the 2 depends uponthe increase of Ir-SP level of the spinal cord mediated through the opiate receptors.展开更多
Objective:To exploretheserum-freecultureconditionsfordifferentiatingmouseembryonicstemcells(ES cells)intoneuralprecursorcells(NPC)andcomparetheeffectsof humanembryonicfibroblasts(HEF)as thefeederlayer of ES withthatof...Objective:To exploretheserum-freecultureconditionsfordifferentiatingmouseembryonicstemcells(ES cells)intoneuralprecursorcells(NPC)andcomparetheeffectsof humanembryonicfibroblasts(HEF)as thefeederlayer of ES withthatof mouseembryonicfibroblasts(MEF)in vitro.Methods:MouseES cellswereculturedin or notin feederlayer cellsmediumcontainingor notleukemiainhibitoryfactorto suppresstheirdifferentiation.Immunocytochemicalmethod was usedto identifyNPCby detectingnestinantigenandalkalinephosphatase.Results: TheES cellsculturedin HEF werepositiveto alkalinephosphatase.Serum-freemediumallowedthedifferentiationof ES cellsintoNPC.Conclusion:HEFcouldreplaceMEFandkeeptheundifferentiatedconditionof ES cellswithmorebenefits.NPCof highpuritycould be culturedfromEScellsby serum-freeculturemethod.展开更多
Objective: To study the role of nitric oxide (NO) in the inhibition of formalin-induced hyperalgesia aller intraspinal injection of adrenocorticotropic hormone (ATCH) in rats. Methods: NADPH-d histochemistry assay of ...Objective: To study the role of nitric oxide (NO) in the inhibition of formalin-induced hyperalgesia aller intraspinal injection of adrenocorticotropic hormone (ATCH) in rats. Methods: NADPH-d histochemistry assay of the spinal cord, immuno-histochemical staining of c-fos protein (fos) and determination of the content of gama-aminobutyric acid (CABA) of ’ the spinal spinal cord tissue were performed Results: lntraspinal injection,of ACTH significantly inhibited the increase of nitrie oxide synthetase (NOS)positive . neurons, fos-positive neurons and the elevation of GABA level in the lumbar seg ment of the spinal cord which could be partially reversed with the adminnistration of 10 μmol L-arginine (Arg)Conclu- sion:The decrease of NO synthesis might play a part in the inhibition of formalin -induced hyperalgesia after intraspinal injec- tion of ACTH展开更多
文摘Immunoreactive substance P(Ir-SP)level,and substance P likeimmunoreactivity(SP-Li)in the spinal cord were observed with radioimmunoassay andimmunohistochemistry in rats after they were given an intraperitoneal injection of mor-phine(7.5mg/kg)or electroacupunctured(3V and 3Hz)on the “Jiaji point”.It wasfound that the pain threshold(PT),Ir-SP level and SP-Li in the dorsal horn of the spi-nal cord were more significantly increased in the animals after the administration ofmorphine or electroacupuncture than in the control(P【0.05～0.01).The combined effectsof morphine and electropacupuncture were even more powerful than either of the agentswas administered singly.Naloxone could block the analgesic effect and the elevation ofIr-SP due to morphine or electroacupuncture.The findings suggest that there is a synergismbe tween morphine and electroacupuncture and the analgesic effect of the 2 depends uponthe increase of Ir-SP level of the spinal cord mediated through the opiate receptors.
文摘Objective:To exploretheserum-freecultureconditionsfordifferentiatingmouseembryonicstemcells(ES cells)intoneuralprecursorcells(NPC)andcomparetheeffectsof humanembryonicfibroblasts(HEF)as thefeederlayer of ES withthatof mouseembryonicfibroblasts(MEF)in vitro.Methods:MouseES cellswereculturedin or notin feederlayer cellsmediumcontainingor notleukemiainhibitoryfactorto suppresstheirdifferentiation.Immunocytochemicalmethod was usedto identifyNPCby detectingnestinantigenandalkalinephosphatase.Results: TheES cellsculturedin HEF werepositiveto alkalinephosphatase.Serum-freemediumallowedthedifferentiationof ES cellsintoNPC.Conclusion:HEFcouldreplaceMEFandkeeptheundifferentiatedconditionof ES cellswithmorebenefits.NPCof highpuritycould be culturedfromEScellsby serum-freeculturemethod.
文摘Objective: To study the role of nitric oxide (NO) in the inhibition of formalin-induced hyperalgesia aller intraspinal injection of adrenocorticotropic hormone (ATCH) in rats. Methods: NADPH-d histochemistry assay of the spinal cord, immuno-histochemical staining of c-fos protein (fos) and determination of the content of gama-aminobutyric acid (CABA) of ’ the spinal spinal cord tissue were performed Results: lntraspinal injection,of ACTH significantly inhibited the increase of nitrie oxide synthetase (NOS)positive . neurons, fos-positive neurons and the elevation of GABA level in the lumbar seg ment of the spinal cord which could be partially reversed with the adminnistration of 10 μmol L-arginine (Arg)Conclu- sion:The decrease of NO synthesis might play a part in the inhibition of formalin -induced hyperalgesia after intraspinal injec- tion of ACTH
