The interactions of two kinds of water-soluble porphyrins, Cu (NEAE) andH2PPS, with DNA have been investigated by microcajorimetry and UV spectroscopy.The experimental results show that Cu (NEAE) interact with dsDNA i...The interactions of two kinds of water-soluble porphyrins, Cu (NEAE) andH2PPS, with DNA have been investigated by microcajorimetry and UV spectroscopy.The experimental results show that Cu (NEAE) interact with dsDNA in the outside-boundmode, its binding number is 9～10 base pairs and its binding enthalpy △H＝-24．4kJ．mol-1.There are large enthalpy changes when Cu (NEAE) react with ssDNA. H2PPS can notreact with both dsDNA and ssDAN, which demonstrats that the coulombic force is one ofthe important factors affecting the interactions of porphyrins with DNA.展开更多
This paper reports the thermodynamics of reversible oxygen binding to a dicobalt di(meso-tetraphenylporphyrin) linked by a diamido- aliphatic chain in N, Ndimethylformamide solution. Thermodynamic values(standard stat...This paper reports the thermodynamics of reversible oxygen binding to a dicobalt di(meso-tetraphenylporphyrin) linked by a diamido- aliphatic chain in N, Ndimethylformamide solution. Thermodynamic values(standard state 101325 Pa O2): △G (298K)=-3.1kJ mol-1, △H (298K)=-37.1kJ·mol-1, △S (298K)=-114 J.mol-1K-1,Co:O2=1:1. The ESR parameters of the dioxygen complex in DMF at 133K are as follows: g=2.000, g=2.046, A= 15G, A =22G, The pressure of dioxygen necessary for half-oxygenation of the system (P1/2 =42.7kPa at 288K) is comparable with that for cobalt substituted human hemoglobin at T state.展开更多
文摘The interactions of two kinds of water-soluble porphyrins, Cu (NEAE) andH2PPS, with DNA have been investigated by microcajorimetry and UV spectroscopy.The experimental results show that Cu (NEAE) interact with dsDNA in the outside-boundmode, its binding number is 9～10 base pairs and its binding enthalpy △H＝-24．4kJ．mol-1.There are large enthalpy changes when Cu (NEAE) react with ssDNA. H2PPS can notreact with both dsDNA and ssDAN, which demonstrats that the coulombic force is one ofthe important factors affecting the interactions of porphyrins with DNA.
文摘This paper reports the thermodynamics of reversible oxygen binding to a dicobalt di(meso-tetraphenylporphyrin) linked by a diamido- aliphatic chain in N, Ndimethylformamide solution. Thermodynamic values(standard state 101325 Pa O2): △G (298K)=-3.1kJ mol-1, △H (298K)=-37.1kJ·mol-1, △S (298K)=-114 J.mol-1K-1,Co:O2=1:1. The ESR parameters of the dioxygen complex in DMF at 133K are as follows: g=2.000, g=2.046, A= 15G, A =22G, The pressure of dioxygen necessary for half-oxygenation of the system (P1/2 =42.7kPa at 288K) is comparable with that for cobalt substituted human hemoglobin at T state.
