运用大型有限元分析软件Abaqus,对船用钢板EH36平板CO2气体保护焊的温度场进行三维动态分析。以8 mm EH36平板为研究对象,考虑材料的热物理性能和对流、辐射散热的影响,建立Y型坡口对接两层两道焊模型,利用过渡网格技术划分网格,采用双...运用大型有限元分析软件Abaqus,对船用钢板EH36平板CO2气体保护焊的温度场进行三维动态分析。以8 mm EH36平板为研究对象,考虑材料的热物理性能和对流、辐射散热的影响,建立Y型坡口对接两层两道焊模型,利用过渡网格技术划分网格,采用双椭球热源模型和"单元生死"技术实现焊缝填充的动态过程。得到EH36温度场的分布规律,并研究层间冷却时间对焊接温度场的影响。展开更多
Objective To investigate the cleavage activities of ribozyme RCP in blocking HBV gene expression and replication in eukaryotic cells. Methods: The recombinant plasmid PCR/pSVL which can transiently express ribozyme RC...Objective To investigate the cleavage activities of ribozyme RCP in blocking HBV gene expression and replication in eukaryotic cells. Methods: The recombinant plasmid PCR/pSVL which can transiently express ribozyme RCP in eukaryotic cells was constructed and introduced into HepG2215 cell line with the technique of lipofectamine-mediated gene transfer. The vector pSVL transfection group served as the control. HB-sAg and HBsAg from culture medium of the cells were tested with solid-phase radioimmunoassay. Results:The cpm obtained from the medium samples showed that the inhibition rate of ribozyme RCP for HBsAg andHBeAg was 26. 7% and 24. 8% respectively in this transient expression system. Conclusion:Hammerhead ribozyme RCP can inhibit to some extent the expression of HBV gene.展开更多
文摘运用大型有限元分析软件Abaqus,对船用钢板EH36平板CO2气体保护焊的温度场进行三维动态分析。以8 mm EH36平板为研究对象,考虑材料的热物理性能和对流、辐射散热的影响,建立Y型坡口对接两层两道焊模型,利用过渡网格技术划分网格,采用双椭球热源模型和"单元生死"技术实现焊缝填充的动态过程。得到EH36温度场的分布规律,并研究层间冷却时间对焊接温度场的影响。
文摘Objective To investigate the cleavage activities of ribozyme RCP in blocking HBV gene expression and replication in eukaryotic cells. Methods: The recombinant plasmid PCR/pSVL which can transiently express ribozyme RCP in eukaryotic cells was constructed and introduced into HepG2215 cell line with the technique of lipofectamine-mediated gene transfer. The vector pSVL transfection group served as the control. HB-sAg and HBsAg from culture medium of the cells were tested with solid-phase radioimmunoassay. Results:The cpm obtained from the medium samples showed that the inhibition rate of ribozyme RCP for HBsAg andHBeAg was 26. 7% and 24. 8% respectively in this transient expression system. Conclusion:Hammerhead ribozyme RCP can inhibit to some extent the expression of HBV gene.
