Objective:To analyze the p16INK4a genomic alteration and expression status in 3 human pancreatic carcinoma cell lines with different potential of metastasis. Methods:Using PCR-SSCP, Dot-blot and immunohistochemistry, ...Objective:To analyze the p16INK4a genomic alteration and expression status in 3 human pancreatic carcinoma cell lines with different potential of metastasis. Methods:Using PCR-SSCP, Dot-blot and immunohistochemistry, the p16INK4a genomic mutation and expression were analyzed on DNA, mRNA and protein levels in 3 human pancreatic carcinoma cell lines Patu8902, Patu8988 and SW1990, which had different potential of metastasis. Results: (1) On DNA level: there was no deletion of p16INK4a Exon Ⅰ in 3cell lines; p16INK4a Exon Ⅱ was only deleted in Patu8902 while no deletion in Patu8988 and SW1990. No insertion, microdeletion and point mutation were found in the 3 cell lines. (2) On RNA level: the expression of p16INK4a protein was negative in Patu8902, low expressed in SW1990, but highly expressed in Patu8988.(3) On protein level: P16 protein was strongly stained in Patu8988, much lower in SW1990, but not stained in Patu8902. Conclusion:The genomic type and expression of p16INK4a are quite different in 3 pancreatic carcinoma cell lines which have different potential of metastasis. It is suggested that genomic homozygous deletion and low expression of mRNA might relate to the potential of metastasis of pancreatic cell lines. In other words, dysfunction of p16INK4a might be an important mechanism in the metastasis of pancreatic carcinoma.展开更多
Objective： To evaluate the clinical value of laparoscopic inguinal hernia repair in hernioplasty and simultaneous cholecystectomy. Methods： Twenty-eight patients with symptomatic chronic calculous cholecystitis and ...Objective： To evaluate the clinical value of laparoscopic inguinal hernia repair in hernioplasty and simultaneous cholecystectomy. Methods： Twenty-eight patients with symptomatic chronic calculous cholecystitis and synchronous unilateral primary inguinal hernia were performed combined surgery between October 2001 and March 2005. Of them, 10 cases underwent laparoscopic totally extraperitoneal mesh hernia repair （TEP） and laparoscopic cholecystectomy （LC）, 3 cases underwent laparoscopic transabdominal preperitoneal mesh hernia repair （TAPP） and LC, and 15 cases underwent LC and open tension-free hernia repair. Results： All the procedures were performed successfully, 2 patients occurred urinary retention in LC＋open group and 1 patient occurred scrotum seroma in LC＋TEP procedures. During the 6 to 24 months＇ follow-up, no hernia recurrences occurred in all patients. There were 6 patients （40%） in LC ＋open group had discomfort pain in the inguinal region and lasted 1 to 3 months. The operating time was longer in the totally laparoscopic group （TEP＋LC and TAPP＋LC） （104±31 min） than in the LC＋open group （80±28 min） （P〈0. 05）. The intensity of postoperative pain at rest was greater in the LC＋open group at 24 h （P〈0.05） and 48 h （P〈0.05）. No differences between the 2 groups were found in the mean operating costs and oral intake of the postoperative period. But the time resume to walking （2.9 vs 1. 8 d） （P〈0.01） and the mean hospital stay （8.2 vs 4.6 d） （P〈0.001） was longer in the LC＋open group than in the totally laparoscopic group. Conclusion： In the same operating costs, the totally laparoscopic precedure has more advantages of low postoperative pain, quicker resume to walking and less hospital stay than open tension-free hernia repair in hernioplasty and simultaneous LC. Thus, the totally laparoscopic approach is considered to be advantage of the hernioplasty and simultaneous LC.展开更多
文摘Objective:To analyze the p16INK4a genomic alteration and expression status in 3 human pancreatic carcinoma cell lines with different potential of metastasis. Methods:Using PCR-SSCP, Dot-blot and immunohistochemistry, the p16INK4a genomic mutation and expression were analyzed on DNA, mRNA and protein levels in 3 human pancreatic carcinoma cell lines Patu8902, Patu8988 and SW1990, which had different potential of metastasis. Results: (1) On DNA level: there was no deletion of p16INK4a Exon Ⅰ in 3cell lines; p16INK4a Exon Ⅱ was only deleted in Patu8902 while no deletion in Patu8988 and SW1990. No insertion, microdeletion and point mutation were found in the 3 cell lines. (2) On RNA level: the expression of p16INK4a protein was negative in Patu8902, low expressed in SW1990, but highly expressed in Patu8988.(3) On protein level: P16 protein was strongly stained in Patu8988, much lower in SW1990, but not stained in Patu8902. Conclusion:The genomic type and expression of p16INK4a are quite different in 3 pancreatic carcinoma cell lines which have different potential of metastasis. It is suggested that genomic homozygous deletion and low expression of mRNA might relate to the potential of metastasis of pancreatic cell lines. In other words, dysfunction of p16INK4a might be an important mechanism in the metastasis of pancreatic carcinoma.
文摘Objective： To evaluate the clinical value of laparoscopic inguinal hernia repair in hernioplasty and simultaneous cholecystectomy. Methods： Twenty-eight patients with symptomatic chronic calculous cholecystitis and synchronous unilateral primary inguinal hernia were performed combined surgery between October 2001 and March 2005. Of them, 10 cases underwent laparoscopic totally extraperitoneal mesh hernia repair （TEP） and laparoscopic cholecystectomy （LC）, 3 cases underwent laparoscopic transabdominal preperitoneal mesh hernia repair （TAPP） and LC, and 15 cases underwent LC and open tension-free hernia repair. Results： All the procedures were performed successfully, 2 patients occurred urinary retention in LC＋open group and 1 patient occurred scrotum seroma in LC＋TEP procedures. During the 6 to 24 months＇ follow-up, no hernia recurrences occurred in all patients. There were 6 patients （40%） in LC ＋open group had discomfort pain in the inguinal region and lasted 1 to 3 months. The operating time was longer in the totally laparoscopic group （TEP＋LC and TAPP＋LC） （104±31 min） than in the LC＋open group （80±28 min） （P〈0. 05）. The intensity of postoperative pain at rest was greater in the LC＋open group at 24 h （P〈0.05） and 48 h （P〈0.05）. No differences between the 2 groups were found in the mean operating costs and oral intake of the postoperative period. But the time resume to walking （2.9 vs 1. 8 d） （P〈0.01） and the mean hospital stay （8.2 vs 4.6 d） （P〈0.001） was longer in the LC＋open group than in the totally laparoscopic group. Conclusion： In the same operating costs, the totally laparoscopic precedure has more advantages of low postoperative pain, quicker resume to walking and less hospital stay than open tension-free hernia repair in hernioplasty and simultaneous LC. Thus, the totally laparoscopic approach is considered to be advantage of the hernioplasty and simultaneous LC.
