Objective:To clone prothymosin α which has important biological functions and to analyze its sequence. Methods:huPTMA α cDNA was cloned from poly(A) + RNA of the healthy Chinese peripheral blood monocyte cells by RT...Objective:To clone prothymosin α which has important biological functions and to analyze its sequence. Methods:huPTMA α cDNA was cloned from poly(A) + RNA of the healthy Chinese peripheral blood monocyte cells by RT-PCR and the sequence was analyzed. Results:It was a new subtype of huPTMA α and has 86.4% homology with Genbank data. Conclusion:We find a new gene from the healthy Chinese, and it is named and accepted by Genbank.展开更多
Objective: To study the chemotactic superfamily genes expression profiling of macrophage line U937 treated with monocyte chemoattractant protein-1 (MCP-1) using gene chip technique. Methods: Total RNA from macrophage ...Objective: To study the chemotactic superfamily genes expression profiling of macrophage line U937 treated with monocyte chemoattractant protein-1 (MCP-1) using gene chip technique. Methods: Total RNA from macrophage line U937 (as control) and U937 with MCP-1 was extracted, made reverse transcript to cDNA and tested with gene expression chip HO2 human. Results: Some chemotactic-related gene expressions were changed in all analyzed genes. Regulated upon activation, normal T cell expressed and secreted (RANTES) was up-regulated over 2-fold and 7 chemotactic-related genes (CCR2, CCR5, CCL16, GROβ, GROγ, IL-8 and granulocyte chemotactic protein 2) were down-regulated over 2-fold in MCP-1 treated U937 cells at mRNA level. Conclusion: MCP-1 can influence some chemokines and receptors expression in macrophage in vitro, in which MCP-1 mainly down-regulates the chemotactic genes expression of those influencing neutrophilic granulocyte (GROβ, GROγ, IL-8 and granulocyte chemotactic protein 2). Another novel finding is that it can also down-regulate the mRNA level of CCR5, which plays a critical role in many disorders and illnesses.展开更多
文摘Objective:To clone prothymosin α which has important biological functions and to analyze its sequence. Methods:huPTMA α cDNA was cloned from poly(A) + RNA of the healthy Chinese peripheral blood monocyte cells by RT-PCR and the sequence was analyzed. Results:It was a new subtype of huPTMA α and has 86.4% homology with Genbank data. Conclusion:We find a new gene from the healthy Chinese, and it is named and accepted by Genbank.
文摘Objective: To study the chemotactic superfamily genes expression profiling of macrophage line U937 treated with monocyte chemoattractant protein-1 (MCP-1) using gene chip technique. Methods: Total RNA from macrophage line U937 (as control) and U937 with MCP-1 was extracted, made reverse transcript to cDNA and tested with gene expression chip HO2 human. Results: Some chemotactic-related gene expressions were changed in all analyzed genes. Regulated upon activation, normal T cell expressed and secreted (RANTES) was up-regulated over 2-fold and 7 chemotactic-related genes (CCR2, CCR5, CCL16, GROβ, GROγ, IL-8 and granulocyte chemotactic protein 2) were down-regulated over 2-fold in MCP-1 treated U937 cells at mRNA level. Conclusion: MCP-1 can influence some chemokines and receptors expression in macrophage in vitro, in which MCP-1 mainly down-regulates the chemotactic genes expression of those influencing neutrophilic granulocyte (GROβ, GROγ, IL-8 and granulocyte chemotactic protein 2). Another novel finding is that it can also down-regulate the mRNA level of CCR5, which plays a critical role in many disorders and illnesses.
