objective: To construct a human Bax eukaryotic expression vector and detect its expression in hu man drug resistant gastric cancer cells. Methods: Bax gene was first inserted into polyclonal sites of pBK CMV vector. T...objective: To construct a human Bax eukaryotic expression vector and detect its expression in hu man drug resistant gastric cancer cells. Methods: Bax gene was first inserted into polyclonal sites of pBK CMV vector. Then the recombinant plasmid vector was trans fected into vincristine resistant human gastric cancer cells SGC7901/VCR by lipofectamine. G418 was used to select resistant clones. Lastly, Western blot was used to observe the expression of Bax protein. Results: Recombinant plasmids were successfully con structed. When the plasmids were trans fected into SGC7901/VCR cellsl stable trans fected cell clones were obtained 30 d after G418 selection. The Western blotting demonstrated a significant expression of Bax protein in pBK-Bax cDNA trans fected cells. Conclusion: This construction of Bax gene transfectant will be conducive to a further study of the role of Bax in gastric cancer multi-drug-resistance.展开更多
文摘objective: To construct a human Bax eukaryotic expression vector and detect its expression in hu man drug resistant gastric cancer cells. Methods: Bax gene was first inserted into polyclonal sites of pBK CMV vector. Then the recombinant plasmid vector was trans fected into vincristine resistant human gastric cancer cells SGC7901/VCR by lipofectamine. G418 was used to select resistant clones. Lastly, Western blot was used to observe the expression of Bax protein. Results: Recombinant plasmids were successfully con structed. When the plasmids were trans fected into SGC7901/VCR cellsl stable trans fected cell clones were obtained 30 d after G418 selection. The Western blotting demonstrated a significant expression of Bax protein in pBK-Bax cDNA trans fected cells. Conclusion: This construction of Bax gene transfectant will be conducive to a further study of the role of Bax in gastric cancer multi-drug-resistance.
