In order to study the physiological mechanism of mail sterile Chestnut during male inflorescence abortion, the activities of SOD, POD, CAT and the content of MDA of male sterile variety of `Fulaiwuhua’ and male ferti...In order to study the physiological mechanism of mail sterile Chestnut during male inflorescence abortion, the activities of SOD, POD, CAT and the content of MDA of male sterile variety of `Fulaiwuhua’ and male fertile variety of `Dahongpao’ during the development of male inflorescence were measured. The results showed that the SOD activities of mail sterile variety were higher than that of male fertile variety during the development of male inflorescence. The POD activities of mail sterile variety were higher than male fertile variety at the early stage of male inflorescence development, and rose obviously when the male inflorescence was dropping. The CAT activities of mail sterile variety were obviously higher than that of male fertile variety and their changes trend was the same. The MDA contents of mail sterile variety were obviously lower than that of male fertile variety except the male inflorescence was dropping. It was suggested that the destroying of active oxygen eliminating system might be the physiological reason of male inflorescence abortion.展开更多
’Dashi Early Ripening’ plums were treated with 0.1,0.5,1 μL·L-1 1-methylcyclopropene(1-MCP)at ambient temperature for 12 h following harvest to evaluate the effectiveness of 1-MCP in extending the shelf life.T...’Dashi Early Ripening’ plums were treated with 0.1,0.5,1 μL·L-1 1-methylcyclopropene(1-MCP)at ambient temperature for 12 h following harvest to evaluate the effectiveness of 1-MCP in extending the shelf life.The fruit firmness,soluble pectin,malondialdehyde(MDA)content,respiration rate,superoxide dismutase(SOD)and catalase(CAT)activities as well as soluble sugar and titratable acidity content of the treated plums were measured over thereafter 12 d during which the plums were stored in ambient temperature.The result showed that the plums treated with 1 μL·L-1 1-MCP remained consumer quality better than that with the other treatments.Their firmness was 249% higher than that of control on the 3rd d of storage,their MDA content was 40% lower than that of control on the 9th d of storage,the titratable acidity was 16.5% higher and the soluble sugar content was 25% higher than that of control at the end of the storage.The treatment with 1 μL·L-1 1-MCP lowered the respiration rate and soluble pectin content,and retarded the respiration peak in comparison with control.The treatment also maintained relative higher SOD,CAT activities.It was concluded that 1 μL·L-1 1-MCP treatment was efficient for extending the shelf life and maintaining the quality of ’Dashi Early Ripening’ plums.展开更多
为建立罗丹明B(rhodamine B)的免疫分析方法,采用戊二醛法,将半抗原罗丹明123与载体牛血清白蛋白(BSA)及卵清蛋白(OVA)偶联制备免疫抗原R123-B S A和包被抗原R123-OVA。经动物免疫实验证实人工抗原合成,并制备抗罗丹明123的多克隆抗体,...为建立罗丹明B(rhodamine B)的免疫分析方法,采用戊二醛法,将半抗原罗丹明123与载体牛血清白蛋白(BSA)及卵清蛋白(OVA)偶联制备免疫抗原R123-B S A和包被抗原R123-OVA。经动物免疫实验证实人工抗原合成,并制备抗罗丹明123的多克隆抗体,此抗体同时能够检测食品中的罗丹明B,且最低检测限为0.001ng/mL。展开更多
文摘In order to study the physiological mechanism of mail sterile Chestnut during male inflorescence abortion, the activities of SOD, POD, CAT and the content of MDA of male sterile variety of `Fulaiwuhua’ and male fertile variety of `Dahongpao’ during the development of male inflorescence were measured. The results showed that the SOD activities of mail sterile variety were higher than that of male fertile variety during the development of male inflorescence. The POD activities of mail sterile variety were higher than male fertile variety at the early stage of male inflorescence development, and rose obviously when the male inflorescence was dropping. The CAT activities of mail sterile variety were obviously higher than that of male fertile variety and their changes trend was the same. The MDA contents of mail sterile variety were obviously lower than that of male fertile variety except the male inflorescence was dropping. It was suggested that the destroying of active oxygen eliminating system might be the physiological reason of male inflorescence abortion.
文摘’Dashi Early Ripening’ plums were treated with 0.1,0.5,1 μL·L-1 1-methylcyclopropene(1-MCP)at ambient temperature for 12 h following harvest to evaluate the effectiveness of 1-MCP in extending the shelf life.The fruit firmness,soluble pectin,malondialdehyde(MDA)content,respiration rate,superoxide dismutase(SOD)and catalase(CAT)activities as well as soluble sugar and titratable acidity content of the treated plums were measured over thereafter 12 d during which the plums were stored in ambient temperature.The result showed that the plums treated with 1 μL·L-1 1-MCP remained consumer quality better than that with the other treatments.Their firmness was 249% higher than that of control on the 3rd d of storage,their MDA content was 40% lower than that of control on the 9th d of storage,the titratable acidity was 16.5% higher and the soluble sugar content was 25% higher than that of control at the end of the storage.The treatment with 1 μL·L-1 1-MCP lowered the respiration rate and soluble pectin content,and retarded the respiration peak in comparison with control.The treatment also maintained relative higher SOD,CAT activities.It was concluded that 1 μL·L-1 1-MCP treatment was efficient for extending the shelf life and maintaining the quality of ’Dashi Early Ripening’ plums.
文摘为建立罗丹明B(rhodamine B)的免疫分析方法,采用戊二醛法,将半抗原罗丹明123与载体牛血清白蛋白(BSA)及卵清蛋白(OVA)偶联制备免疫抗原R123-B S A和包被抗原R123-OVA。经动物免疫实验证实人工抗原合成,并制备抗罗丹明123的多克隆抗体,此抗体同时能够检测食品中的罗丹明B,且最低检测限为0.001ng/mL。
