Objective:To investigate the alteration of serum soluble fms-like tyrosine kinase receptor 1 (sFlt-1), the possible cause in preeclampsia, the relationship between altered sFlt-1 and the development of pregnancy. Meth...Objective:To investigate the alteration of serum soluble fms-like tyrosine kinase receptor 1 (sFlt-1), the possible cause in preeclampsia, the relationship between altered sFlt-1 and the development of pregnancy. Methods: Semi-quantitative RT-PCR was carried out to detect the level of sFlt-1 mRNA in placental tissue of 10 preeclampsia (study group) and 10 normotensive pregnancies (control group); ELISA was used to detect the serum level of sFlt-1 in peripheral venous blood and uterine venous blood in control group (n=35) and study group (n=35), and furthermore to detect the volume of peripheral venous blood sFlt-1 in early, middle and later pregnancies. Results: sFlt-1 mRNA of placental tissue was significantly higher in preeclampsia than that in normal pregnancy. The serum level of sFlt-1 of peripheral vein in preeclampsia was higher than that in normal pregnancy ([ 5639.6±3190.5] pg/ml vs[ 2194.4±635.8 ] pg/ml, P<0.01) , so did the serum sFlt-1 of uterine vein in preeclampsia and control group ([7673.3±2295.8] pg/ml vs[ 3056.6±784.6] pg/ml, P<0.01), indicating that the volume of sFlt-1 of uterine vein blood was significantly higher than that of peripheral venous blood (P<0.01). The serum level of sFlt-1 in early, middle and later pregnancy were (31.7±19.6) pg/ml (n=10), (994.0±302.1) pg/ml (n=10), (2194.4±635.8) pg/ml (n=35), respectively, showing that the level of sFlt-1 in peripheral venous blood increasingly elevated with the development of pregnancy (P<0.01). Conclusion: Preeclampsia is associated with increased level of sFlt-1 which might be largely from placenta during pregnancy.展开更多
Objective: To investigate the contribution of placental leptin to the serum levels in preeclampsia and the effect of hypoxia on synthesis of placental leptin. Methods: Fifteen preeclamptic women and 20 normotensive pr...Objective: To investigate the contribution of placental leptin to the serum levels in preeclampsia and the effect of hypoxia on synthesis of placental leptin. Methods: Fifteen preeclamptic women and 20 normotensive pregnant women were recruited in present study. Leptin concentrations in peripheral venous blood samples and uterine venous blood samples were measured by radioimmunoassay. Eight cases of normal human term placental villi were cultured either in normaxia （21%O2） or in hypoxia （2%O2） followed by determining leptin in the culture medium by radioimmunoassay. Results: Leptin concentrations were significantly higher in preeclamptic women than in normotensive pregnant women, both in the peripheral vein （[23.29±12.87] μg/L vs [13.87±5.57] μg/L, P<0.01） and uterine vein （[16.44±8.62] μg/L vs [11.21±4.20] μg/L, P<0.05）. Leptin concentrations were significantly higher in the peripheral vein than in uterine vein, both in the preeclamptic （P<0.01） and in normotensive pregnant women （P<0.01）. Concentrations of leptin in the culture medium were significantly increased in hypoxia than in normoxia （P<0.05）. Conclusion: The pathogenesis of preeclampsia may be associated with an increase of maternal serum leptin and placenta leptin, and hypoxia in placenta may be an important factor that results in preeclamptic placenta to produce more leptin. Placenta is not the principal source of the serum leptin in the preeclamptic women or normotensive pregnant women.展开更多
To identify gene expression profiling in epithelial ovarian cancer and to explore its correlation with histopathology characterization and prognosis.Gene expression profiles were generated from 10 hu- man ovarian froz...To identify gene expression profiling in epithelial ovarian cancer and to explore its correlation with histopathology characterization and prognosis.Gene expression profiles were generated from 10 hu- man ovarian frozen tissue specimens using Agilent Human 1A microarrays.Strikingly.clear differences of gene expression patterns were observed in ovarian cancer as compared to normal tissues.Unique gene pro files were observed in moderately and poorly differentiated epithelial ovarian cancer.It is concluded that different histopathology characterization likely exists extensive molecular heterogeneity.展开更多
文摘Objective:To investigate the alteration of serum soluble fms-like tyrosine kinase receptor 1 (sFlt-1), the possible cause in preeclampsia, the relationship between altered sFlt-1 and the development of pregnancy. Methods: Semi-quantitative RT-PCR was carried out to detect the level of sFlt-1 mRNA in placental tissue of 10 preeclampsia (study group) and 10 normotensive pregnancies (control group); ELISA was used to detect the serum level of sFlt-1 in peripheral venous blood and uterine venous blood in control group (n=35) and study group (n=35), and furthermore to detect the volume of peripheral venous blood sFlt-1 in early, middle and later pregnancies. Results: sFlt-1 mRNA of placental tissue was significantly higher in preeclampsia than that in normal pregnancy. The serum level of sFlt-1 of peripheral vein in preeclampsia was higher than that in normal pregnancy ([ 5639.6±3190.5] pg/ml vs[ 2194.4±635.8 ] pg/ml, P<0.01) , so did the serum sFlt-1 of uterine vein in preeclampsia and control group ([7673.3±2295.8] pg/ml vs[ 3056.6±784.6] pg/ml, P<0.01), indicating that the volume of sFlt-1 of uterine vein blood was significantly higher than that of peripheral venous blood (P<0.01). The serum level of sFlt-1 in early, middle and later pregnancy were (31.7±19.6) pg/ml (n=10), (994.0±302.1) pg/ml (n=10), (2194.4±635.8) pg/ml (n=35), respectively, showing that the level of sFlt-1 in peripheral venous blood increasingly elevated with the development of pregnancy (P<0.01). Conclusion: Preeclampsia is associated with increased level of sFlt-1 which might be largely from placenta during pregnancy.
文摘Objective: To investigate the contribution of placental leptin to the serum levels in preeclampsia and the effect of hypoxia on synthesis of placental leptin. Methods: Fifteen preeclamptic women and 20 normotensive pregnant women were recruited in present study. Leptin concentrations in peripheral venous blood samples and uterine venous blood samples were measured by radioimmunoassay. Eight cases of normal human term placental villi were cultured either in normaxia （21%O2） or in hypoxia （2%O2） followed by determining leptin in the culture medium by radioimmunoassay. Results: Leptin concentrations were significantly higher in preeclamptic women than in normotensive pregnant women, both in the peripheral vein （[23.29±12.87] μg/L vs [13.87±5.57] μg/L, P<0.01） and uterine vein （[16.44±8.62] μg/L vs [11.21±4.20] μg/L, P<0.05）. Leptin concentrations were significantly higher in the peripheral vein than in uterine vein, both in the preeclamptic （P<0.01） and in normotensive pregnant women （P<0.01）. Concentrations of leptin in the culture medium were significantly increased in hypoxia than in normoxia （P<0.05）. Conclusion: The pathogenesis of preeclampsia may be associated with an increase of maternal serum leptin and placenta leptin, and hypoxia in placenta may be an important factor that results in preeclamptic placenta to produce more leptin. Placenta is not the principal source of the serum leptin in the preeclamptic women or normotensive pregnant women.
基金Foundation of Science and Technology Pro-gram of Shaanxi province[No.2007k09-09(1)]
文摘To identify gene expression profiling in epithelial ovarian cancer and to explore its correlation with histopathology characterization and prognosis.Gene expression profiles were generated from 10 hu- man ovarian frozen tissue specimens using Agilent Human 1A microarrays.Strikingly.clear differences of gene expression patterns were observed in ovarian cancer as compared to normal tissues.Unique gene pro files were observed in moderately and poorly differentiated epithelial ovarian cancer.It is concluded that different histopathology characterization likely exists extensive molecular heterogeneity.
