Adeno-associated virus(AAV) is a single-stranded linear defective parvovirus. Using the model proposed by Cavalier-Smith for replication of ss-DNA virus. the linear fragment of inverted terminal repeats(ITRs) from PAA...Adeno-associated virus(AAV) is a single-stranded linear defective parvovirus. Using the model proposed by Cavalier-Smith for replication of ss-DNA virus. the linear fragment of inverted terminal repeats(ITRs) from PAAV/neo was isolated, denatured and refolded over. The daughter DNA was synthesized by using 3'-OH terminus as a primer with the new strand covalently linked to the parental strand. After the palindromic structure with neomycin resistance gene controlled by SV40 early promoter into eukaryotic cells. Southern blot showed that the molecule of hairpin structure could facilitate the copies into mammalian cells in vitro. The experiment supports the Cavalier-Smith's replication model of ss-DNA virus and provides another new system for DNA transduction.展开更多
文摘Adeno-associated virus(AAV) is a single-stranded linear defective parvovirus. Using the model proposed by Cavalier-Smith for replication of ss-DNA virus. the linear fragment of inverted terminal repeats(ITRs) from PAAV/neo was isolated, denatured and refolded over. The daughter DNA was synthesized by using 3'-OH terminus as a primer with the new strand covalently linked to the parental strand. After the palindromic structure with neomycin resistance gene controlled by SV40 early promoter into eukaryotic cells. Southern blot showed that the molecule of hairpin structure could facilitate the copies into mammalian cells in vitro. The experiment supports the Cavalier-Smith's replication model of ss-DNA virus and provides another new system for DNA transduction.
