The metabolites and metabolic passways of nitrazepam in rat were confirmed.Wistar rats were feed a pill of nitrazepam,24 h urine reactions were collected.After β-Glucuronidase hydrolysis of the urine samples,the frac...The metabolites and metabolic passways of nitrazepam in rat were confirmed.Wistar rats were feed a pill of nitrazepam,24 h urine reactions were collected.After β-Glucuronidase hydrolysis of the urine samples,the fractions were extracted by Oasis HLB3cc solid-phase column and analyzed by gas chromatography-mass spectrometry with DB-35 MS column.7-Acetylaminonitrazepam,7-aminonitrazepam and 2-amino-5-nitrophenylphenylmethanone were identified as nitrazepam metabolites.The results suggested that two metabolic passways for nitrazepam may be operative in rat.The first passway leads to the corresponding 7-aminonimetazepam in which the amino group is subsequently acetylated.The second passway is open the parent compounds rings to 2-amino-5-nitrophenylphenylmethanone.Nitrazepam was metabolized quickly in rats and 7-acetylaminonitrazepam were the main metabolites in urine.展开更多
A rapid method for the determination of morphine-type drugs in urine was established by SPE/GC-MS,and was applied in the real samples with satisfactory results.
To establish a convenient and efficient SPME/GC-MS method for the determination of terbufos in mulberry leaf.Samples were extracted by HS/SPME and analyzed by GC-MS.Mean recoveries ranged from(90±5.7)%.The method...To establish a convenient and efficient SPME/GC-MS method for the determination of terbufos in mulberry leaf.Samples were extracted by HS/SPME and analyzed by GC-MS.Mean recoveries ranged from(90±5.7)%.The method was simple,rapid,accurate,and was suitable for qualititative analysis of terbufos residue in mulberry leaf.展开更多
Tramadol and its four metabolites O-desmethyltramadol,N-desmethyltramadol,N,O-didesmethyltramadol,Hydroxy tramadol were identified by GC-MS(EI,PCI).The contents and kinds of tramadol and its metabolites were compared ...Tramadol and its four metabolites O-desmethyltramadol,N-desmethyltramadol,N,O-didesmethyltramadol,Hydroxy tramadol were identified by GC-MS(EI,PCI).The contents and kinds of tramadol and its metabolites were compared between human urine and rat urine.The identical metabolites were found in human urine and rat urine but different contents.Some of the tramadol and its metabolites existing as conjugative and the others existing as educts in body were found after enzymatic hydrolysis.展开更多
文摘The metabolites and metabolic passways of nitrazepam in rat were confirmed.Wistar rats were feed a pill of nitrazepam,24 h urine reactions were collected.After β-Glucuronidase hydrolysis of the urine samples,the fractions were extracted by Oasis HLB3cc solid-phase column and analyzed by gas chromatography-mass spectrometry with DB-35 MS column.7-Acetylaminonitrazepam,7-aminonitrazepam and 2-amino-5-nitrophenylphenylmethanone were identified as nitrazepam metabolites.The results suggested that two metabolic passways for nitrazepam may be operative in rat.The first passway leads to the corresponding 7-aminonimetazepam in which the amino group is subsequently acetylated.The second passway is open the parent compounds rings to 2-amino-5-nitrophenylphenylmethanone.Nitrazepam was metabolized quickly in rats and 7-acetylaminonitrazepam were the main metabolites in urine.
文摘A rapid method for the determination of morphine-type drugs in urine was established by SPE/GC-MS,and was applied in the real samples with satisfactory results.
文摘To establish a convenient and efficient SPME/GC-MS method for the determination of terbufos in mulberry leaf.Samples were extracted by HS/SPME and analyzed by GC-MS.Mean recoveries ranged from(90±5.7)%.The method was simple,rapid,accurate,and was suitable for qualititative analysis of terbufos residue in mulberry leaf.
文摘Tramadol and its four metabolites O-desmethyltramadol,N-desmethyltramadol,N,O-didesmethyltramadol,Hydroxy tramadol were identified by GC-MS(EI,PCI).The contents and kinds of tramadol and its metabolites were compared between human urine and rat urine.The identical metabolites were found in human urine and rat urine but different contents.Some of the tramadol and its metabolites existing as conjugative and the others existing as educts in body were found after enzymatic hydrolysis.
