目的观察血管活性肠肽（vasoactive intestinal peptide,VIP）对脂多糖（lipopolysaccharides,LPS）应激的小鼠成纤维细胞髓样细胞表达触发受体-2（TREM-2）表达的影响,并初步探讨其信号转导通路。方法利用LPS腹腔注射建立急性肺损伤（...目的观察血管活性肠肽（vasoactive intestinal peptide,VIP）对脂多糖（lipopolysaccharides,LPS）应激的小鼠成纤维细胞髓样细胞表达触发受体-2（TREM-2）表达的影响,并初步探讨其信号转导通路。方法利用LPS腹腔注射建立急性肺损伤（ALI）小鼠模型;采用VIP慢病毒气管滴注,q PCR检测肺组织TREM-2的表达。选用q PCR和流式细胞术检测VIP对LPS应激的小鼠成纤维细胞TREM-2表达的影响;并观察PKC信号通路阻断剂（H-7）、PKA信号通路阻断剂（H-89）、MAPK信号通路阻断剂（PD98059）和Ca M信号通路阻断剂（W-7）对VIP调控TREM-2表达的影响。结果 ALI时小鼠肺组织TREM-2 m RNA表达降低,而VIP可上调肺组织TREM-2 m RNA的表达。LPS下调小鼠成纤维细胞TREM-2 m RNA的表达,VIP可呈时间依赖性上调TREM-2 m RNA的表达（0、3、6、12和24 h）,且在6 h达到峰值;并呈剂量相关性上调TREM-2 m RNA的表达（10^（-10）、10^（-9）、10^（-8）和10^（-7）mol/L）,以10^（-8）mol/L作用最明显。VIP对LPS应激6 h增加小鼠成纤维细胞TREM-2 m RNA和蛋白表达的效应可被H-7、PD98059以及W-7所阻断。结论 LPS下调小鼠成纤维细胞TREM-2的表达,而VIP可上调LPS应激的小鼠成纤维细胞TREM-2 m RNA的表达,其胞内信号转导途径可能与PKC、MAPK及Ca M有关。展开更多
Concentration of heavy metals in blood and urine of rabbit after inhaling three different kinds of cigarette was studied through the animal passive smoking pattern. The samples were prepared by nitric acid solution di...Concentration of heavy metals in blood and urine of rabbit after inhaling three different kinds of cigarette was studied through the animal passive smoking pattern. The samples were prepared by nitric acid solution digestion and determination of seven kinds of heavy metals including Hg, Se, Sn, Pb, Cd, Ni and Cr was performed by inductively coupled plasma-atomic emission spectrometry （ICP-AES）. The ICP-AES method was established with good precision and accuracy, relative standard deviation （n=6） was between 2.9% and 5.9%, and the recovery was in the range of 95.0%-104.2%. Concentration of six heavy metals increases in some extent in blood and urine after period of smoking and the increasing of heavy metals in blood and urine all shows time dependence. Significantly higher heavy metal levels are observed in the blood and urine of the cigarette inhaling rabbits in the exposed group. The concentration of six kinds of heavy metals in the blood of the rabbit increases after 16 weeks exposing to cigarette smoking. Three times of rig, ten times of Se and trace amount ofPb, Cd, Ni and Cr are detected in the blood after 16 weeks of smoking. For urine samples, about three times of Hg, two times of Se, five times of Pb and trace amount of Cd are detected after 16 weeks of inhalation of cigarette. Comparatively, higher concentration of heavy metals are detected after inhaling of Nise cigarette.展开更多
文摘目的观察血管活性肠肽（vasoactive intestinal peptide,VIP）对脂多糖（lipopolysaccharides,LPS）应激的小鼠成纤维细胞髓样细胞表达触发受体-2（TREM-2）表达的影响,并初步探讨其信号转导通路。方法利用LPS腹腔注射建立急性肺损伤（ALI）小鼠模型;采用VIP慢病毒气管滴注,q PCR检测肺组织TREM-2的表达。选用q PCR和流式细胞术检测VIP对LPS应激的小鼠成纤维细胞TREM-2表达的影响;并观察PKC信号通路阻断剂（H-7）、PKA信号通路阻断剂（H-89）、MAPK信号通路阻断剂（PD98059）和Ca M信号通路阻断剂（W-7）对VIP调控TREM-2表达的影响。结果 ALI时小鼠肺组织TREM-2 m RNA表达降低,而VIP可上调肺组织TREM-2 m RNA的表达。LPS下调小鼠成纤维细胞TREM-2 m RNA的表达,VIP可呈时间依赖性上调TREM-2 m RNA的表达（0、3、6、12和24 h）,且在6 h达到峰值;并呈剂量相关性上调TREM-2 m RNA的表达（10^（-10）、10^（-9）、10^（-8）和10^（-7）mol/L）,以10^（-8）mol/L作用最明显。VIP对LPS应激6 h增加小鼠成纤维细胞TREM-2 m RNA和蛋白表达的效应可被H-7、PD98059以及W-7所阻断。结论 LPS下调小鼠成纤维细胞TREM-2的表达,而VIP可上调LPS应激的小鼠成纤维细胞TREM-2 m RNA的表达,其胞内信号转导途径可能与PKC、MAPK及Ca M有关。
基金Project(11JJ5053) supported by the Provincial Natural Science Foundation of Hunan Province,China
文摘Concentration of heavy metals in blood and urine of rabbit after inhaling three different kinds of cigarette was studied through the animal passive smoking pattern. The samples were prepared by nitric acid solution digestion and determination of seven kinds of heavy metals including Hg, Se, Sn, Pb, Cd, Ni and Cr was performed by inductively coupled plasma-atomic emission spectrometry （ICP-AES）. The ICP-AES method was established with good precision and accuracy, relative standard deviation （n=6） was between 2.9% and 5.9%, and the recovery was in the range of 95.0%-104.2%. Concentration of six heavy metals increases in some extent in blood and urine after period of smoking and the increasing of heavy metals in blood and urine all shows time dependence. Significantly higher heavy metal levels are observed in the blood and urine of the cigarette inhaling rabbits in the exposed group. The concentration of six kinds of heavy metals in the blood of the rabbit increases after 16 weeks exposing to cigarette smoking. Three times of rig, ten times of Se and trace amount ofPb, Cd, Ni and Cr are detected in the blood after 16 weeks of smoking. For urine samples, about three times of Hg, two times of Se, five times of Pb and trace amount of Cd are detected after 16 weeks of inhalation of cigarette. Comparatively, higher concentration of heavy metals are detected after inhaling of Nise cigarette.
