To study the chemical constituents,the ethanol extract of dried stem of Akebia quinata(Thunb.) Decne.was seperated by dichloromethane,n-butanol in sequence.The n-butanol extract was further isolated by D101 macroporou...To study the chemical constituents,the ethanol extract of dried stem of Akebia quinata(Thunb.) Decne.was seperated by dichloromethane,n-butanol in sequence.The n-butanol extract was further isolated by D101 macroporous resin and silica gel column chromatography.A new triterpene with two known triterpenes were isolated,their structures were identified as 3α,24-dihydroxy-30-norolean-12,20(29)-dien-28-oic acid(Ⅰ),3α,24,29-trihydroxyolean-12-en-28-oic acid(Ⅱ),2α,3β,23-trihydroxyolean-12-en-28-oic acid(Ⅲ).The new compound Ⅱ was nominated as quinatic stem acid.The structures were identified and elucidated by physicochemical properties and spectral analysis.展开更多
目的:建立冬虫夏草药材中麦角甾醇的RP-HPLC含量测定方法。方法:冬虫夏草药材粉末经甲醇超声提取后,用RP-HPLC测定。采用Phenomenex Luna C18(250mm×4.6mm,5μm)色谱柱,流动相为甲醇-水(98:2);流速为1mL·min-1;检测波长为282n...目的:建立冬虫夏草药材中麦角甾醇的RP-HPLC含量测定方法。方法:冬虫夏草药材粉末经甲醇超声提取后,用RP-HPLC测定。采用Phenomenex Luna C18(250mm×4.6mm,5μm)色谱柱,流动相为甲醇-水(98:2);流速为1mL·min-1;检测波长为282nm。结果:麦角甾醇进样量在0.04272～0.4272μg与峰面积呈良好的线性关系(r=0.9999);麦角甾醇的平均回收率为98.7%(n=6)。结论:采用建立的RP-HPLC方法测定冬虫夏草药材中麦角甾醇的含量,方法灵敏、快速、准确。展开更多
文摘To study the chemical constituents,the ethanol extract of dried stem of Akebia quinata(Thunb.) Decne.was seperated by dichloromethane,n-butanol in sequence.The n-butanol extract was further isolated by D101 macroporous resin and silica gel column chromatography.A new triterpene with two known triterpenes were isolated,their structures were identified as 3α,24-dihydroxy-30-norolean-12,20(29)-dien-28-oic acid(Ⅰ),3α,24,29-trihydroxyolean-12-en-28-oic acid(Ⅱ),2α,3β,23-trihydroxyolean-12-en-28-oic acid(Ⅲ).The new compound Ⅱ was nominated as quinatic stem acid.The structures were identified and elucidated by physicochemical properties and spectral analysis.
文摘目的:建立冬虫夏草药材中麦角甾醇的RP-HPLC含量测定方法。方法:冬虫夏草药材粉末经甲醇超声提取后,用RP-HPLC测定。采用Phenomenex Luna C18(250mm×4.6mm,5μm)色谱柱,流动相为甲醇-水(98:2);流速为1mL·min-1;检测波长为282nm。结果:麦角甾醇进样量在0.04272～0.4272μg与峰面积呈良好的线性关系(r=0.9999);麦角甾醇的平均回收率为98.7%(n=6)。结论:采用建立的RP-HPLC方法测定冬虫夏草药材中麦角甾醇的含量,方法灵敏、快速、准确。
