To reduce Ni ion release and improve biocompatibility of NiTi alloy, the cathodic plasma electrolytic deposition （CPED） technique was used to fabricate ceramic coating onto a NiTi alloy surface. The formation of a c...To reduce Ni ion release and improve biocompatibility of NiTi alloy, the cathodic plasma electrolytic deposition （CPED） technique was used to fabricate ceramic coating onto a NiTi alloy surface. The formation of a coating with a rough and micro-textured surface was confirmed by X-ray diffraction, scanning electron microscopy, and energy-dispersive X-ray spectroscopy, re- spectively. An inductively coupled plasma mass spectrometry test showed that the formed coating significantly reduced the release of Ni ions from the NiTi alloy in simulated body fluid. The in- fluence of CPED treated NiTi substrates on the biological behaviors of osteoblasts, including cell adhesion, cell viability, and osteogenic differentiation function （alkaline phosphatase）, was inves- tigated in vitro. Immunofluorescence staining of nuclei revealed that the CPED treated NiTi alloy was favorable for cell growth. Osteoblasts on CPED modified NiTi alloy showed greater cell viability than those for the native NiTi substrate after 4 and 7 days cultures. More importantly, osteoblasts cultured onto a modified NiTi sample displayed significantly higher differentiation lev-els of alkaline phosphatase. The results suggested that surface functionalization of NiTi alloy with ceramic coating via the CPED technique was beneficial for cell proliferation and differentiation. The approach presented here is useful for NiTi implants to enhance bone osseointegration and reduce Ni ion release in vitro.展开更多
基金supported by China Ministry of Science and Technology (973 project No. 2009CB930000)National Natural Science Foundation of China (Nos. 11032012 and 51173216)+3 种基金Fok Ying Tung Education Foundation (121035)Natural Science Foundation of Chongqing Municipal Government (CSTC2011jjjq10004 and CSTC2012gg-yyjs10023)Fundamental Research Funds for the Central Universities (Nos. CDJXS10232211, CDJZR11230005)the sharing fund of Chongqing University's large-scale equipment (Nos. 2011063046,2011063047)
文摘To reduce Ni ion release and improve biocompatibility of NiTi alloy, the cathodic plasma electrolytic deposition （CPED） technique was used to fabricate ceramic coating onto a NiTi alloy surface. The formation of a coating with a rough and micro-textured surface was confirmed by X-ray diffraction, scanning electron microscopy, and energy-dispersive X-ray spectroscopy, re- spectively. An inductively coupled plasma mass spectrometry test showed that the formed coating significantly reduced the release of Ni ions from the NiTi alloy in simulated body fluid. The in- fluence of CPED treated NiTi substrates on the biological behaviors of osteoblasts, including cell adhesion, cell viability, and osteogenic differentiation function （alkaline phosphatase）, was inves- tigated in vitro. Immunofluorescence staining of nuclei revealed that the CPED treated NiTi alloy was favorable for cell growth. Osteoblasts on CPED modified NiTi alloy showed greater cell viability than those for the native NiTi substrate after 4 and 7 days cultures. More importantly, osteoblasts cultured onto a modified NiTi sample displayed significantly higher differentiation lev-els of alkaline phosphatase. The results suggested that surface functionalization of NiTi alloy with ceramic coating via the CPED technique was beneficial for cell proliferation and differentiation. The approach presented here is useful for NiTi implants to enhance bone osseointegration and reduce Ni ion release in vitro.
