In this paper, two anionic two phase systems, sodium dodecyl sulfate(SDS) dodecyl triethylammonium bromide(C 12 NE) and sodium perfluorooctanoate (SPFO) C 12 NE, were established. A two phase system with a clear inter...In this paper, two anionic two phase systems, sodium dodecyl sulfate(SDS) dodecyl triethylammonium bromide(C 12 NE) and sodium perfluorooctanoate (SPFO) C 12 NE, were established. A two phase system with a clear interfacial boundary formed spontaneously under certain surfactant concentrations and molar ratios, respectively for the above two mixtures. Phase separation conditions and their properties were studied. Extractions of dyes and porphyrin compounds were performed. The results show that hydrophobic molecules are easily extracted into the surfactant rich phase of SPFO C 12 NE or into the “aqueous phase” of SDS C 12 NE. Positively charged porphyrins are extracted with higher extraction efficiencies than negatively charged porphyrins. The extraction results for charged porphyrins are opposite to those extracted with cationic two phase of C 12 NE SDS mixture, indicating that anionic two phase systems are complementary to the cationic two phase systems.展开更多
Room temperature phosphorescence(RTP) signal intensity of eosin-ITC on a polyamide membrane(PM) was very low, but the signal was increased obviously once some inorganic salts were added into the system at λ ex/λ em=...Room temperature phosphorescence(RTP) signal intensity of eosin-ITC on a polyamide membrane(PM) was very low, but the signal was increased obviously once some inorganic salts were added into the system at λ ex/λ em=532 nm/685 nm. The enhancement effect of ⅡA element ions(such as Ca 2+, Sr 2+ and Ba 2+) on RTP of eosin-ITC is much obvious than that of Pb 2+ and Hg 2+ used constantly as an outer heavy atom perturber in phosphorimetry. Based on that, a new solid substrate room temperature phosphorescence immunoassay(SS-RTP-IA) for the determination of the human IgG was proposed to demonstrate the feasibility using eosin-ITC as phosphorescent marker. It was found that a good linear relationship with the ΔI P was obtained when the amount of human IgG in the range from 6.25 to 400 pg/spot(corresponding to the mass concentration: 15.63 ng/mL—1 μg/L, sample volume of 0.4 μL/spot). The regression {equation} of working curve can be expressed as ΔI p=3.062+0.00864x(pg)(n=7, r=0.996), The detection limit calculated as 3S b/k is 0.394 pg/spot. The method was applied directly to the determination of IgG in human serum with a satisfying result. These results show that eosin-ITC can be used conveniently as a RTP marker of high sensitivity.展开更多
文摘In this paper, two anionic two phase systems, sodium dodecyl sulfate(SDS) dodecyl triethylammonium bromide(C 12 NE) and sodium perfluorooctanoate (SPFO) C 12 NE, were established. A two phase system with a clear interfacial boundary formed spontaneously under certain surfactant concentrations and molar ratios, respectively for the above two mixtures. Phase separation conditions and their properties were studied. Extractions of dyes and porphyrin compounds were performed. The results show that hydrophobic molecules are easily extracted into the surfactant rich phase of SPFO C 12 NE or into the “aqueous phase” of SDS C 12 NE. Positively charged porphyrins are extracted with higher extraction efficiencies than negatively charged porphyrins. The extraction results for charged porphyrins are opposite to those extracted with cationic two phase of C 12 NE SDS mixture, indicating that anionic two phase systems are complementary to the cationic two phase systems.
文摘Room temperature phosphorescence(RTP) signal intensity of eosin-ITC on a polyamide membrane(PM) was very low, but the signal was increased obviously once some inorganic salts were added into the system at λ ex/λ em=532 nm/685 nm. The enhancement effect of ⅡA element ions(such as Ca 2+, Sr 2+ and Ba 2+) on RTP of eosin-ITC is much obvious than that of Pb 2+ and Hg 2+ used constantly as an outer heavy atom perturber in phosphorimetry. Based on that, a new solid substrate room temperature phosphorescence immunoassay(SS-RTP-IA) for the determination of the human IgG was proposed to demonstrate the feasibility using eosin-ITC as phosphorescent marker. It was found that a good linear relationship with the ΔI P was obtained when the amount of human IgG in the range from 6.25 to 400 pg/spot(corresponding to the mass concentration: 15.63 ng/mL—1 μg/L, sample volume of 0.4 μL/spot). The regression {equation} of working curve can be expressed as ΔI p=3.062+0.00864x(pg)(n=7, r=0.996), The detection limit calculated as 3S b/k is 0.394 pg/spot. The method was applied directly to the determination of IgG in human serum with a satisfying result. These results show that eosin-ITC can be used conveniently as a RTP marker of high sensitivity.
