The interaction of arsenazo Ⅲ with serum albumin and the optimum condition for the determination of protein were investigated by spectrophotometry.In a Clark—Lubs buffer solution of pH 3.0,arsenazo Ⅲ interacts with...The interaction of arsenazo Ⅲ with serum albumin and the optimum condition for the determination of protein were investigated by spectrophotometry.In a Clark—Lubs buffer solution of pH 3.0,arsenazo Ⅲ interacts with bovine serum albumin and they form a compound with an maximum absorption wavelength of 550 nm in the presence of surfactant OP.The apparent molar absorptivity of the compound is ε550nm=4.36×105L·mol-1·cm-1.Beer’s law is obeyed over 0～80mg·L-1of protein.The proteins in human serum were determined by the proposed methed with satisfactory results.展开更多
文摘The interaction of arsenazo Ⅲ with serum albumin and the optimum condition for the determination of protein were investigated by spectrophotometry.In a Clark—Lubs buffer solution of pH 3.0,arsenazo Ⅲ interacts with bovine serum albumin and they form a compound with an maximum absorption wavelength of 550 nm in the presence of surfactant OP.The apparent molar absorptivity of the compound is ε550nm=4.36×105L·mol-1·cm-1.Beer’s law is obeyed over 0～80mg·L-1of protein.The proteins in human serum were determined by the proposed methed with satisfactory results.
