Objective: To explore the relationship between expression of cathepsin D (Cath-D) and tumor cell proliferative activity and prognosis in osteosarcoma. Methods: Expression of proliferating cell nuclear antigen (PCNA) a...Objective: To explore the relationship between expression of cathepsin D (Cath-D) and tumor cell proliferative activity and prognosis in osteosarcoma. Methods: Expression of proliferating cell nuclear antigen (PCNA) and Cath-D in tumor cells were semiquantitatively anajyzed with immunohistochemistry in a consecutiv e prospective series of 85 cases of osteosarcoma. Results: In 85 cases of osteosarcoma, the Cath-D LI was ranged from 6% - 98. 5% and PCNA LI from 9% - 98%. There was no correlation between the Cath-D LI and the patients’ age, sex, tumor size and Dahlin’s classification. However, significant correlation was found between the Cath-D LI and new WHO classification in 1993, Price’s grade and the PCNA LI. Furthermore, Kaplan-Meier analysis showed that the patients with a Cath-D LI less than 66% had a better survival than those with that more than 66%. Conclusion: There is significant relationship between Cath-D expression and survival, suggesting that Cath-D LI might be a useful prognostic indicator in osteosarcoma.展开更多
One hundren and twenty cases of osteosarcoma (OS) were studied histopathologically and immunohistochemically. It is now generally accepted that the demonstration of neoplastic osteoid is not essential for the diagnosi...One hundren and twenty cases of osteosarcoma (OS) were studied histopathologically and immunohistochemically. It is now generally accepted that the demonstration of neoplastic osteoid is not essential for the diagnosis of osteosarcoma but the microscopic features of sarcomatous stroma, the heteromorphism of osteoblasts and the sarcomatous background of anaplastic giant cells and chondrosarcomatous and fibrosarcomatous tissues are of diagnostic significance. Re-examination of all our tissue specimens revealed that typical neoplastic osteoid was ouly found in 83% of our series. The differential diagnosis between neoplastic osteoid and pseudo-osteoid or fibrillar hyalinized collagen is discussed. Forty-eight surgical specimens were stained with polyclonal actin, monoclonal BMP (bone morphogenetic protein), vimentin, collagen type IV (C-IV) and UEA-1 with immunohistochemical ABC method. It was found that when there was positive expression of monoclonal BMP, vimentin, C-IV,UEA-1 and polyclonal actin, that of monoclonal BMP, vimentin, UEA-1 and/or polyclonal actin, or that of monoclonal BMP, vimentin and/or polyclonal actin, it was of diagnostic significance for osteosarcoma. However, the 5 markers were of no value to differentiate fibrillar hyalinized collagen from osteoid stroma. It is believed that appropriate combination of the immunohistochemical markers is imperative to promote the accuracy of the pathological diagnosis of osteosarcoma and its differential diagnosis.展开更多
文摘Objective: To explore the relationship between expression of cathepsin D (Cath-D) and tumor cell proliferative activity and prognosis in osteosarcoma. Methods: Expression of proliferating cell nuclear antigen (PCNA) and Cath-D in tumor cells were semiquantitatively anajyzed with immunohistochemistry in a consecutiv e prospective series of 85 cases of osteosarcoma. Results: In 85 cases of osteosarcoma, the Cath-D LI was ranged from 6% - 98. 5% and PCNA LI from 9% - 98%. There was no correlation between the Cath-D LI and the patients’ age, sex, tumor size and Dahlin’s classification. However, significant correlation was found between the Cath-D LI and new WHO classification in 1993, Price’s grade and the PCNA LI. Furthermore, Kaplan-Meier analysis showed that the patients with a Cath-D LI less than 66% had a better survival than those with that more than 66%. Conclusion: There is significant relationship between Cath-D expression and survival, suggesting that Cath-D LI might be a useful prognostic indicator in osteosarcoma.
文摘One hundren and twenty cases of osteosarcoma (OS) were studied histopathologically and immunohistochemically. It is now generally accepted that the demonstration of neoplastic osteoid is not essential for the diagnosis of osteosarcoma but the microscopic features of sarcomatous stroma, the heteromorphism of osteoblasts and the sarcomatous background of anaplastic giant cells and chondrosarcomatous and fibrosarcomatous tissues are of diagnostic significance. Re-examination of all our tissue specimens revealed that typical neoplastic osteoid was ouly found in 83% of our series. The differential diagnosis between neoplastic osteoid and pseudo-osteoid or fibrillar hyalinized collagen is discussed. Forty-eight surgical specimens were stained with polyclonal actin, monoclonal BMP (bone morphogenetic protein), vimentin, collagen type IV (C-IV) and UEA-1 with immunohistochemical ABC method. It was found that when there was positive expression of monoclonal BMP, vimentin, C-IV,UEA-1 and polyclonal actin, that of monoclonal BMP, vimentin, UEA-1 and/or polyclonal actin, or that of monoclonal BMP, vimentin and/or polyclonal actin, it was of diagnostic significance for osteosarcoma. However, the 5 markers were of no value to differentiate fibrillar hyalinized collagen from osteoid stroma. It is believed that appropriate combination of the immunohistochemical markers is imperative to promote the accuracy of the pathological diagnosis of osteosarcoma and its differential diagnosis.
