Genomic DNA was successfully extracted from leaf tissues of two rattan species,Calamus simplificifolius and Daemonorops margaritae,using a modified CTAB method.This method could remove phenolic compounds,polysaccharid...Genomic DNA was successfully extracted from leaf tissues of two rattan species,Calamus simplificifolius and Daemonorops margaritae,using a modified CTAB method.This method could remove phenolic compounds,polysaccharides and proteins,and the quality and quantity of DNA extracted were reliably characterized.In addition,the reaction composition and amplification program for RAPD analysis were developed with GeneAmp PCR System 2700 (Applied Biosystem Co.).This study provided a technological basis for future molecular studies in rattan and the related species.展开更多
文摘Genomic DNA was successfully extracted from leaf tissues of two rattan species,Calamus simplificifolius and Daemonorops margaritae,using a modified CTAB method.This method could remove phenolic compounds,polysaccharides and proteins,and the quality and quantity of DNA extracted were reliably characterized.In addition,the reaction composition and amplification program for RAPD analysis were developed with GeneAmp PCR System 2700 (Applied Biosystem Co.).This study provided a technological basis for future molecular studies in rattan and the related species.
