The continued application of electron microscopy as an adjunct procedure for the diagnosis of human diseases has been questioned in light of the explosive development and refinement of immunophenotyping, cytogenetic a...The continued application of electron microscopy as an adjunct procedure for the diagnosis of human diseases has been questioned in light of the explosive development and refinement of immunophenotyping, cytogenetic and molecular techniques (nucleic acid hybridization, molecular genetics, and the polymerase chain reaction). Accumulated fact demonstrated lately that a combination of immunohistochemistry and molecular techniques could not identify every disease and some diagnostic problems went unanswered. And immunohistochemistry sometimes produced results that are entirely convincing but incorrect. It is confirmed by our experience and that of many other investigations that electron microscopy can be applied to human disease diagnosis still as a powerful tool. It can help diagnose nonimmunoreactive diseases, nonneoplastic or neoplastic, clarify diagnoses in cases with complex and confusing immunophenotypes, promote understanding of the differentiation of new or controversial entities, and , as a result, enhance light microscopy diagnostic expertise.展开更多
文摘The continued application of electron microscopy as an adjunct procedure for the diagnosis of human diseases has been questioned in light of the explosive development and refinement of immunophenotyping, cytogenetic and molecular techniques (nucleic acid hybridization, molecular genetics, and the polymerase chain reaction). Accumulated fact demonstrated lately that a combination of immunohistochemistry and molecular techniques could not identify every disease and some diagnostic problems went unanswered. And immunohistochemistry sometimes produced results that are entirely convincing but incorrect. It is confirmed by our experience and that of many other investigations that electron microscopy can be applied to human disease diagnosis still as a powerful tool. It can help diagnose nonimmunoreactive diseases, nonneoplastic or neoplastic, clarify diagnoses in cases with complex and confusing immunophenotypes, promote understanding of the differentiation of new or controversial entities, and , as a result, enhance light microscopy diagnostic expertise.
