In order to compare the contemporary and genetic variation effect on Arabidop-sis thaliana treated with N+ implantation and 7-ray radiation, the authors did some statistical comparison on the germinating rate and the ...In order to compare the contemporary and genetic variation effect on Arabidop-sis thaliana treated with N+ implantation and 7-ray radiation, the authors did some statistical comparison on the germinating rate and the development period, and analyzed the content of soluble proteins, the activity of some enzymes, isoenzymes profile, and along with the variation in genome DNA of two generations by RAPD. With N+ implantation there was an analogical 'saddle model' relationship between doses and the plant development, soluble proteins, the activity of some enzymes and isoenzymes profile. A certain connection might exist between the similar dose-effect relations among enzymes activity, isoenzymes profile and content of soluble proteins. Maybe, there also exists a certain connection between the mutants of development period and that of DNA variations, between the hereditability of the effect of N+ implantation on the isoenzymes, the activities of enzymes and the hereditability of DNA variations. So it is presumed that the implanted ions, maybe, have participated in metabolism process of organism including that of genome DNA, to consequently affect vital process, such as the changes of gene structure, gene expression manner and gene repair mechanism, and finally result in mutation on phenotype and molecular level. Furthermore, the results definitely showed that mutagenic mechanism induced by N+ implantation is very complicated and is much different from that induced by traditional 7-ray radiation.展开更多
Ion implantation, as a new biophysically mutagenic technique, has shown a great potential for crop breeding. By analyzing polymorphisms of genomic DNA through RAPD-based DNA analysis, we compared the frequency and eff...Ion implantation, as a new biophysically mutagenic technique, has shown a great potential for crop breeding. By analyzing polymorphisms of genomic DNA through RAPD-based DNA analysis, we compared the frequency and efficiency of somatic and germ-line mutations of Arabidopsis thaliana treated with N^＋ ion implantation and γ-rays radiation. Our data support the following conclusions： （1） N^＋ ion implantation can induce a much wider spectrum of mutations than γ-rays radiation does; （2） Unlike the linear correlation between the doses and their effect in γ-rays radiation, the dose-effect correlation in N^＋ ion implantation is nonlinear; （3） Like γ-rays radiation, both somatic and germ-line mutations could be induced by N^＋ ion implantation; and （4） RAPD deletion patterns are usually seen in N^＋ ion implantation induced mutation.展开更多
基金The project supported by the National Natural Science Foundation of China (No. 10435020)the project of backbone youth teachers in high Colleges of Education Department of China Central Government
文摘In order to compare the contemporary and genetic variation effect on Arabidop-sis thaliana treated with N+ implantation and 7-ray radiation, the authors did some statistical comparison on the germinating rate and the development period, and analyzed the content of soluble proteins, the activity of some enzymes, isoenzymes profile, and along with the variation in genome DNA of two generations by RAPD. With N+ implantation there was an analogical 'saddle model' relationship between doses and the plant development, soluble proteins, the activity of some enzymes and isoenzymes profile. A certain connection might exist between the similar dose-effect relations among enzymes activity, isoenzymes profile and content of soluble proteins. Maybe, there also exists a certain connection between the mutants of development period and that of DNA variations, between the hereditability of the effect of N+ implantation on the isoenzymes, the activities of enzymes and the hereditability of DNA variations. So it is presumed that the implanted ions, maybe, have participated in metabolism process of organism including that of genome DNA, to consequently affect vital process, such as the changes of gene structure, gene expression manner and gene repair mechanism, and finally result in mutation on phenotype and molecular level. Furthermore, the results definitely showed that mutagenic mechanism induced by N+ implantation is very complicated and is much different from that induced by traditional 7-ray radiation.
基金supported by the National Natural Science Foundation of China (Nos. 30570434, 10435020)special funding from the Ministry of Education of China for Backbone Young Teachers at Key Universities
文摘Ion implantation, as a new biophysically mutagenic technique, has shown a great potential for crop breeding. By analyzing polymorphisms of genomic DNA through RAPD-based DNA analysis, we compared the frequency and efficiency of somatic and germ-line mutations of Arabidopsis thaliana treated with N^＋ ion implantation and γ-rays radiation. Our data support the following conclusions： （1） N^＋ ion implantation can induce a much wider spectrum of mutations than γ-rays radiation does; （2） Unlike the linear correlation between the doses and their effect in γ-rays radiation, the dose-effect correlation in N^＋ ion implantation is nonlinear; （3） Like γ-rays radiation, both somatic and germ-line mutations could be induced by N^＋ ion implantation; and （4） RAPD deletion patterns are usually seen in N^＋ ion implantation induced mutation.
