目的研究胃癌患者细胞表面黏附分子CD44在外周血淋巴细胞中的表达及临床意义. 方法根治术的胃癌患者49例.外周血淋巴细胞中CD44的表达应用流式细胞免疫学方法检测,并与正常对照（n=25）进行比较. 结果胃癌患者外周血淋巴细胞中CD44的表...目的研究胃癌患者细胞表面黏附分子CD44在外周血淋巴细胞中的表达及临床意义. 方法根治术的胃癌患者49例.外周血淋巴细胞中CD44的表达应用流式细胞免疫学方法检测,并与正常对照（n=25）进行比较. 结果胃癌患者外周血淋巴细胞中CD44的表达均明显高于正常对照[（437.1±16.3） vs （353.3±18.1）,P＜0.01],手术后2周（381.9±18.7）和12个月（381.5±21.6）表达降低,但仍高于对照（P＜0.05）.胃癌Ⅲ～Ⅳ期CD44的表达明显高于Ⅰ～Ⅱ期[（479.1±24.6） vs （423.2±19.5）,P＜0.05],胃癌有转移患者CD44表达均高于无转移[（462.1±19.2） vs （428.4±19.6）,P＜0.01],但不同组织类型的CD44表达无显著性差异（P＞0.05）. 结论胃癌患者外周血淋巴细胞中CD44的表达水平明显高于正常对照,且手术后可以明显下降,其表达水平与胃癌的进展情况及有无转移、预后有一定关系.展开更多
Objective: To amplify HBV-RNase H gene fragment and express RNase H for further use in the studiesof HBV associated liver diseases. Methods: The encoding gene of HBV-RNase H was separately amplified for thefirst half ...Objective: To amplify HBV-RNase H gene fragment and express RNase H for further use in the studiesof HBV associated liver diseases. Methods: The encoding gene of HBV-RNase H was separately amplified for thefirst half and second half (H1 and H2)by PCR from full length HBV gene and cloned into pT7Blue-T vector.Clones were first screened by digestion with Xbal and HindⅢ enzyme for the correct size, and analyzed further byDNA sequencing. The RNase H1 and H2 fragments isolated from XbaⅠ and HindⅢ digestion products of pT7 BlueRNaseH plasmid were ligated to the GSTag expressing vectors separately, and expressed in E. coli BL21. The expressed proteins were checked by PAGE gel and Western blot. Results: Both H1 and H2 nucleotide seqences wereconsisted with the known genes and the proteins, with correct size, were further confirmed by western blot to bethe GST and RNaseHl or H2 fusion proteins. Conclusion: The successful cloning and expression of HBV-RNase Hwill contribute to further research and application in HBV associated diseases.展开更多
文摘目的研究胃癌患者细胞表面黏附分子CD44在外周血淋巴细胞中的表达及临床意义. 方法根治术的胃癌患者49例.外周血淋巴细胞中CD44的表达应用流式细胞免疫学方法检测,并与正常对照（n=25）进行比较. 结果胃癌患者外周血淋巴细胞中CD44的表达均明显高于正常对照[（437.1±16.3） vs （353.3±18.1）,P＜0.01],手术后2周（381.9±18.7）和12个月（381.5±21.6）表达降低,但仍高于对照（P＜0.05）.胃癌Ⅲ～Ⅳ期CD44的表达明显高于Ⅰ～Ⅱ期[（479.1±24.6） vs （423.2±19.5）,P＜0.05],胃癌有转移患者CD44表达均高于无转移[（462.1±19.2） vs （428.4±19.6）,P＜0.01],但不同组织类型的CD44表达无显著性差异（P＞0.05）. 结论胃癌患者外周血淋巴细胞中CD44的表达水平明显高于正常对照,且手术后可以明显下降,其表达水平与胃癌的进展情况及有无转移、预后有一定关系.
文摘Objective: To amplify HBV-RNase H gene fragment and express RNase H for further use in the studiesof HBV associated liver diseases. Methods: The encoding gene of HBV-RNase H was separately amplified for thefirst half and second half (H1 and H2)by PCR from full length HBV gene and cloned into pT7Blue-T vector.Clones were first screened by digestion with Xbal and HindⅢ enzyme for the correct size, and analyzed further byDNA sequencing. The RNase H1 and H2 fragments isolated from XbaⅠ and HindⅢ digestion products of pT7 BlueRNaseH plasmid were ligated to the GSTag expressing vectors separately, and expressed in E. coli BL21. The expressed proteins were checked by PAGE gel and Western blot. Results: Both H1 and H2 nucleotide seqences wereconsisted with the known genes and the proteins, with correct size, were further confirmed by western blot to bethe GST and RNaseHl or H2 fusion proteins. Conclusion: The successful cloning and expression of HBV-RNase Hwill contribute to further research and application in HBV associated diseases.
