Objective: To investigate whether nitric oxide synthase (NOS) is expressed in T-cell-dependent liver injury initiated by concanavalin A (ConA) in Kunming mice and study the possible effect of nitric oxide(NO) on liver...Objective: To investigate whether nitric oxide synthase (NOS) is expressed in T-cell-dependent liver injury initiated by concanavalin A (ConA) in Kunming mice and study the possible effect of nitric oxide(NO) on liver injury models. Methods: Liver injury in Kunming mice was induced by administration of ConA through tail vein. Expression of NOS in the liver was detected by NADPH diaphorase staining method. The possible effect of NO on liver injury models was obtained by L-NAME injection to suppress synthesis of NO. Results: NOS has a strong expression in hepatocytes after ConA injection, especially in those close to the central vein, while only a weak expression was found in the epithelial cells in control group. Liver injury became more serious when NO synthesis was inhibited by L-NAME, accompanied by great malondialdehyde(MDA) increase in serum and severe intrahepatic vascular thrombosis. Conclusion: NOS markedly expressed in ConA-induced liver injury, which may subsequently promote nitric oxide synthesis. Increasement of nitric oxide has a protective effect on ConA-induced liver injury.展开更多
Objective: To gain more data for the diagnosis and treatment for primary hepatic lymphoma(PHL). Methods: A 32-year-old man was admitted due to fatigue, anorexia, loss of weight and fever. Physical examination was carr...Objective: To gain more data for the diagnosis and treatment for primary hepatic lymphoma(PHL). Methods: A 32-year-old man was admitted due to fatigue, anorexia, loss of weight and fever. Physical examination was carried out and bone marrow aspitation, blood culture, endoscopy of stomach and colon, chest X-ray, renal fumction and liver function test were all done. Results: Physical examination showed slight abdominal tenderness all edema of the legs, and the liver was unpalpable. Alpha- fetoprotein (AFP ) and carcinomoembryonic antigen (CEA ) were negative. Laboratory tests of rheumatic diseases such as ANA and ENA were all normal. Abdominal ultrasonography and computerized tomography showed diffuse low density or diffuse hypoechogenic changes of the liver, there was no obvious mass lesion in the liver. Conclusion: The diagnosis of PHL was affirmed, and the subtype was non-Hodgkin’s lymphoma.展开更多
文摘Objective: To investigate whether nitric oxide synthase (NOS) is expressed in T-cell-dependent liver injury initiated by concanavalin A (ConA) in Kunming mice and study the possible effect of nitric oxide(NO) on liver injury models. Methods: Liver injury in Kunming mice was induced by administration of ConA through tail vein. Expression of NOS in the liver was detected by NADPH diaphorase staining method. The possible effect of NO on liver injury models was obtained by L-NAME injection to suppress synthesis of NO. Results: NOS has a strong expression in hepatocytes after ConA injection, especially in those close to the central vein, while only a weak expression was found in the epithelial cells in control group. Liver injury became more serious when NO synthesis was inhibited by L-NAME, accompanied by great malondialdehyde(MDA) increase in serum and severe intrahepatic vascular thrombosis. Conclusion: NOS markedly expressed in ConA-induced liver injury, which may subsequently promote nitric oxide synthesis. Increasement of nitric oxide has a protective effect on ConA-induced liver injury.
文摘Objective: To gain more data for the diagnosis and treatment for primary hepatic lymphoma(PHL). Methods: A 32-year-old man was admitted due to fatigue, anorexia, loss of weight and fever. Physical examination was carried out and bone marrow aspitation, blood culture, endoscopy of stomach and colon, chest X-ray, renal fumction and liver function test were all done. Results: Physical examination showed slight abdominal tenderness all edema of the legs, and the liver was unpalpable. Alpha- fetoprotein (AFP ) and carcinomoembryonic antigen (CEA ) were negative. Laboratory tests of rheumatic diseases such as ANA and ENA were all normal. Abdominal ultrasonography and computerized tomography showed diffuse low density or diffuse hypoechogenic changes of the liver, there was no obvious mass lesion in the liver. Conclusion: The diagnosis of PHL was affirmed, and the subtype was non-Hodgkin’s lymphoma.
