Objective. To establish a PCR- SSP method for discriminating as many HLA- A* 02 alleles, which could easily be introduced into a routine laboratory. Methods. In this study we typed HLA- A* 02 polymorphisms by a sequen...Objective. To establish a PCR- SSP method for discriminating as many HLA- A* 02 alleles, which could easily be introduced into a routine laboratory. Methods. In this study we typed HLA- A* 02 polymorphisms by a sequence- specific primer (SSP) method, which involved round 1 and round 2 PCR reactions to detect 17 HLA- A* 02 alleles (they are HLA- A* 0201- 0217 alleles) covering exon 2 and exon 3. Results. We have found that DNA sample concentration and purity were the most important variables in determining the quality of the results. For identifying correct band size, the size marker used was important. We noticed that different PCR machines performed differently. By this method, we detected 20 HLA- A* 02 positive genomic DNA samples and found 4 kinds of HLA- A* 02 alleles. They were HLA- A* 0201, 0203, 0206 and 0210. Conclusion. The HLA- A* 02 PCR- SSP method was proven to be a reliable and easily applicable typing method. Our results suggest that the SSP described here provides an optimal HLA- A* 02 typing technique that may be useful in selecting donor- recipient pairs in bone marrow transplantation between unrelated individuals.展开更多
Objective’ Recent studies indicated that incidence of occlusive cardiovascular diseases increased in psoriasis. Changes of serum lipid metabolism may be the cause. Apolipoproteinsplay a central role in lipoprotein me...Objective’ Recent studies indicated that incidence of occlusive cardiovascular diseases increased in psoriasis. Changes of serum lipid metabolism may be the cause. Apolipoproteinsplay a central role in lipoprotein metabolism in serum. The measurement of serum apo levelsmay reveal the characteristics of lipoprotein metabolism in serum in patients with psoriasisand may help to find an answer to the cause of the high incidence of occlusive cardiovasculardiseases in psoriasis.Method. The apo levels of 31 patients with psoriasis vulgaris and 31 sex- and age-matched healthy individuals with normal serum lipid levels were measured by radial immuno-diffusion assay.Results’ The apo C Ⅲ (t= 2.44,P<0. 05) and apo E (t=2. 12, P<0. 05) of psoriat-ic group elevated significantly- The apo C Ⅲ(t= 2.77, P<0.01) of patients less than 40-year-old and apoE (t= 2. 91, P<0. 01) of male patients eIevated significantly. Conclusha. Abndrmal metabolism of lipoprotein in serum exist in psoriasis, especially inmale patients and/or patients less than 40 years old.展开更多
文摘Objective. To establish a PCR- SSP method for discriminating as many HLA- A* 02 alleles, which could easily be introduced into a routine laboratory. Methods. In this study we typed HLA- A* 02 polymorphisms by a sequence- specific primer (SSP) method, which involved round 1 and round 2 PCR reactions to detect 17 HLA- A* 02 alleles (they are HLA- A* 0201- 0217 alleles) covering exon 2 and exon 3. Results. We have found that DNA sample concentration and purity were the most important variables in determining the quality of the results. For identifying correct band size, the size marker used was important. We noticed that different PCR machines performed differently. By this method, we detected 20 HLA- A* 02 positive genomic DNA samples and found 4 kinds of HLA- A* 02 alleles. They were HLA- A* 0201, 0203, 0206 and 0210. Conclusion. The HLA- A* 02 PCR- SSP method was proven to be a reliable and easily applicable typing method. Our results suggest that the SSP described here provides an optimal HLA- A* 02 typing technique that may be useful in selecting donor- recipient pairs in bone marrow transplantation between unrelated individuals.
文摘Objective’ Recent studies indicated that incidence of occlusive cardiovascular diseases increased in psoriasis. Changes of serum lipid metabolism may be the cause. Apolipoproteinsplay a central role in lipoprotein metabolism in serum. The measurement of serum apo levelsmay reveal the characteristics of lipoprotein metabolism in serum in patients with psoriasisand may help to find an answer to the cause of the high incidence of occlusive cardiovasculardiseases in psoriasis.Method. The apo levels of 31 patients with psoriasis vulgaris and 31 sex- and age-matched healthy individuals with normal serum lipid levels were measured by radial immuno-diffusion assay.Results’ The apo C Ⅲ (t= 2.44,P<0. 05) and apo E (t=2. 12, P<0. 05) of psoriat-ic group elevated significantly- The apo C Ⅲ(t= 2.77, P<0.01) of patients less than 40-year-old and apoE (t= 2. 91, P<0. 01) of male patients eIevated significantly. Conclusha. Abndrmal metabolism of lipoprotein in serum exist in psoriasis, especially inmale patients and/or patients less than 40 years old.
