A method for determination of quercetin in 8 kinds of Chinese herbal medicines by capillary electrophoresis(CE) with microwave assisted extraction(MAE) was developed. These medicines include Hypericum periforatum, Sem...A method for determination of quercetin in 8 kinds of Chinese herbal medicines by capillary electrophoresis(CE) with microwave assisted extraction(MAE) was developed. These medicines include Hypericum periforatum, Semen cuscuta, Flos sophorae buds, Flos sophorae flowers, Herba euphorbiae humifusae, Acanthopanax, Ginkgo biloba L. leaves and Rhododendron dauricuml. The method is simple, rapid and with satisfactory recoveries and good reproducibilities.展开更多
A high performance liquid chromatography-tandem mass spectrometric method was established for determination of the nitrofuran metabolites were released.After adjusting pH range from 7.0 to 7.5. Then the analytes were ...A high performance liquid chromatography-tandem mass spectrometric method was established for determination of the nitrofuran metabolites were released.After adjusting pH range from 7.0 to 7.5. Then the analytes were extracted by ethyl acetate identification was achieved by electrospray ionization in positive mode(ESI+) using multiple reaction monitoring.The quantification was performed with external standards.The recoveries of 3-Amino-2-oxazolidinone(AOZ)were in the range of 79.1%-94.8% with spiked levels of 0.5-2 μg/kg.The RSD were less than 8.23%.The limits of detection were 0.10 μg/kg for AOZ.展开更多
目的探讨丹参素对离体鼠肺组织过氧化氢(H2O2)损伤的保护作用及机制。方法制备鼠肺组织精细切片40张,随机分为对照组、损伤组及丹参素低、中、高剂量组。以0.5 ml K-H缓冲液作为孵育液,37℃培养箱内进行孵育。损伤组和丹参素各剂量组制...目的探讨丹参素对离体鼠肺组织过氧化氢(H2O2)损伤的保护作用及机制。方法制备鼠肺组织精细切片40张,随机分为对照组、损伤组及丹参素低、中、高剂量组。以0.5 ml K-H缓冲液作为孵育液,37℃培养箱内进行孵育。损伤组和丹参素各剂量组制备H2O2损伤模型,丹参素低、中、高组分别加入丹参素使其质量浓度分别为2、4、8μg/ml。继续孕育60 min后采用分光光度计测定各组细胞活性及乳酸脱氢酶(LDH)水平。结果丹参素各剂量组细胞活性明显高于损伤组(P<0.05或P<0.01),LDH水平明显低于对照组(P<0.05或P<0.01),呈一定剂量依赖性。结论丹参素能有效减轻肺组织过氧化损伤;其机制可能为清除氧自由基,抑制细胞内钙超载及保护内皮细胞。展开更多
文摘A method for determination of quercetin in 8 kinds of Chinese herbal medicines by capillary electrophoresis(CE) with microwave assisted extraction(MAE) was developed. These medicines include Hypericum periforatum, Semen cuscuta, Flos sophorae buds, Flos sophorae flowers, Herba euphorbiae humifusae, Acanthopanax, Ginkgo biloba L. leaves and Rhododendron dauricuml. The method is simple, rapid and with satisfactory recoveries and good reproducibilities.
文摘A high performance liquid chromatography-tandem mass spectrometric method was established for determination of the nitrofuran metabolites were released.After adjusting pH range from 7.0 to 7.5. Then the analytes were extracted by ethyl acetate identification was achieved by electrospray ionization in positive mode(ESI+) using multiple reaction monitoring.The quantification was performed with external standards.The recoveries of 3-Amino-2-oxazolidinone(AOZ)were in the range of 79.1%-94.8% with spiked levels of 0.5-2 μg/kg.The RSD were less than 8.23%.The limits of detection were 0.10 μg/kg for AOZ.
文摘目的探讨丹参素对离体鼠肺组织过氧化氢(H2O2)损伤的保护作用及机制。方法制备鼠肺组织精细切片40张,随机分为对照组、损伤组及丹参素低、中、高剂量组。以0.5 ml K-H缓冲液作为孵育液,37℃培养箱内进行孵育。损伤组和丹参素各剂量组制备H2O2损伤模型,丹参素低、中、高组分别加入丹参素使其质量浓度分别为2、4、8μg/ml。继续孕育60 min后采用分光光度计测定各组细胞活性及乳酸脱氢酶(LDH)水平。结果丹参素各剂量组细胞活性明显高于损伤组(P<0.05或P<0.01),LDH水平明显低于对照组(P<0.05或P<0.01),呈一定剂量依赖性。结论丹参素能有效减轻肺组织过氧化损伤;其机制可能为清除氧自由基,抑制细胞内钙超载及保护内皮细胞。
