Two experimental patterns of autogenous nerve replantation in situ and centrocentralnerve suture were performed on the rabbit median and ulnar nerves.The results of light andtransmission electron microscopy as well as...Two experimental patterns of autogenous nerve replantation in situ and centrocentralnerve suture were performed on the rabbit median and ulnar nerves.The results of light andtransmission electron microscopy as well as immunohistochemistry showed that autogenous nervereplantation in situ could inhibit the formation of neuroma.The mechanism might be that twoproximal stump axons could touch within the interpolated graft segment.Simple end-to-endnerve suture failed to inhibit neuroma development because of a lack of proper environment forthe touching between two proximal stump axons.展开更多
Objective: To develop China-made or modified UW Solution for organ transplantation. Methods: In 1992, we developed a new solution: NO. 1 Changzheng Multi-organ Preservation Solution(CZ-1 Solution). There were 5 modifi...Objective: To develop China-made or modified UW Solution for organ transplantation. Methods: In 1992, we developed a new solution: NO. 1 Changzheng Multi-organ Preservation Solution(CZ-1 Solution). There were 5 modified aspects as compared with UW solution: (1) replacing hydroxyethylstarch with dextran-40; (2) replacing RAF(raffinose ) with sucrose; (3) removing insulin, penicillin and batricn; (4) adding Ca2+ channel blockers verapamil; (5) further improving the pH value of CZ-1 Solution to 7.45 ±0.1. A series of studies were conducted on how and how long CZ-1 Solution could preserve the organs (such as kidneys, liver, heart, lungs, pancreas, etc. ) of the animals (New Zealand rabbits or SD rats). The morphologic survey was studied by spectrum microscope and electron microscope; the biochemical, physiologic functions and the survival rate during cold prolonged storage were also studied . Results: The effect of CZ-1 was similiar and superior, in some aspects, to that of UW solution in the cold preservation of organs. Conclusion: The controlled study showed that the CZ-1 Solu- tion is as effective as UW Solution and is quite effective for organ preservation. The 24 to 31 h cold storage of cadaveric kidney in transplantation is successful. The advantage of CZ-1 Solution is that it can reduce costs and improve the effect of organ pres- ervation. It can also meet the requirements of future development of transplantation in China.展开更多
Objective: Apoptosis of human mast cell line HMC-1 can be induced by tripterine, a monomer extracted from Tripterygium Wilfordii Hook, f.. This paper is to describe the typical morphologic changes accompanying apoptot...Objective: Apoptosis of human mast cell line HMC-1 can be induced by tripterine, a monomer extracted from Tripterygium Wilfordii Hook, f.. This paper is to describe the typical morphologic changes accompanying apoptotic process, explore the possible mechanism underlying and discuss the significance of using it as hallmark of apoptosis. Methods: On or after exposure to tripterine, cells were analyzed with light microscopy, electromicroscope, flow cytometry, ferminal trans- ferase dUTP nick and end labeling (TFUNEL). Results: Under light microscopy, Cell with patterns like sunflower: Cellular main body surrounded by clusters of small small (resembling the petals of sunflower), could be seen in the apoptotic pro cess. The sunflower-like cells (SLCs), negative to trypan blue staining, wb or without the accompaniment of chromatin condense, could occur as soon as20 min after the addition of tripterine and survive for several hours. Drug-baced SLC was time- and dose-dependent. The apoptosis were significantly correlated with results of TUNEL or PI/FCM in the for & h of induction. The SLCs could be easily discriminated under reverse light microscopy. The number of SLC at different time from different persons was consistent. Conclusion: The results suggested that SLC is a characteristic morphologic chane of apop totic cells, it can be a useful mark for quantifying apoptotic HMC-l cell.展开更多
文摘Two experimental patterns of autogenous nerve replantation in situ and centrocentralnerve suture were performed on the rabbit median and ulnar nerves.The results of light andtransmission electron microscopy as well as immunohistochemistry showed that autogenous nervereplantation in situ could inhibit the formation of neuroma.The mechanism might be that twoproximal stump axons could touch within the interpolated graft segment.Simple end-to-endnerve suture failed to inhibit neuroma development because of a lack of proper environment forthe touching between two proximal stump axons.
文摘Objective: To develop China-made or modified UW Solution for organ transplantation. Methods: In 1992, we developed a new solution: NO. 1 Changzheng Multi-organ Preservation Solution(CZ-1 Solution). There were 5 modified aspects as compared with UW solution: (1) replacing hydroxyethylstarch with dextran-40; (2) replacing RAF(raffinose ) with sucrose; (3) removing insulin, penicillin and batricn; (4) adding Ca2+ channel blockers verapamil; (5) further improving the pH value of CZ-1 Solution to 7.45 ±0.1. A series of studies were conducted on how and how long CZ-1 Solution could preserve the organs (such as kidneys, liver, heart, lungs, pancreas, etc. ) of the animals (New Zealand rabbits or SD rats). The morphologic survey was studied by spectrum microscope and electron microscope; the biochemical, physiologic functions and the survival rate during cold prolonged storage were also studied . Results: The effect of CZ-1 was similiar and superior, in some aspects, to that of UW solution in the cold preservation of organs. Conclusion: The controlled study showed that the CZ-1 Solu- tion is as effective as UW Solution and is quite effective for organ preservation. The 24 to 31 h cold storage of cadaveric kidney in transplantation is successful. The advantage of CZ-1 Solution is that it can reduce costs and improve the effect of organ pres- ervation. It can also meet the requirements of future development of transplantation in China.
文摘Objective: Apoptosis of human mast cell line HMC-1 can be induced by tripterine, a monomer extracted from Tripterygium Wilfordii Hook, f.. This paper is to describe the typical morphologic changes accompanying apoptotic process, explore the possible mechanism underlying and discuss the significance of using it as hallmark of apoptosis. Methods: On or after exposure to tripterine, cells were analyzed with light microscopy, electromicroscope, flow cytometry, ferminal trans- ferase dUTP nick and end labeling (TFUNEL). Results: Under light microscopy, Cell with patterns like sunflower: Cellular main body surrounded by clusters of small small (resembling the petals of sunflower), could be seen in the apoptotic pro cess. The sunflower-like cells (SLCs), negative to trypan blue staining, wb or without the accompaniment of chromatin condense, could occur as soon as20 min after the addition of tripterine and survive for several hours. Drug-baced SLC was time- and dose-dependent. The apoptosis were significantly correlated with results of TUNEL or PI/FCM in the for & h of induction. The SLCs could be easily discriminated under reverse light microscopy. The number of SLC at different time from different persons was consistent. Conclusion: The results suggested that SLC is a characteristic morphologic chane of apop totic cells, it can be a useful mark for quantifying apoptotic HMC-l cell.
