The samples of minks from several main mink farming areas were detected by the methods of counter immunoelectrophoresis(CIEP)and polymerase chain reaction (PCR)and some data about Aleutian mink disease parvovirus(ADV)...The samples of minks from several main mink farming areas were detected by the methods of counter immunoelectrophoresis(CIEP)and polymerase chain reaction (PCR)and some data about Aleutian mink disease parvovirus(ADV)infection in farmed mink was obtained. Partial VP2 genes,which include a hypervariable region, of 16 ADV strains isolated were separately amplified by PCR and sequenced. Comparison and analysis of these genes sequences and the related sequences of ADV-G,ADV-Utah1 and ADV-K that reported in the USA and European had been completed. The result shows that : firstly, the closed relationship exists between domestic ADV isolates and foreign ones,and the domestic AD maybe introduced from foreign countries. Secondly, the ADV isolates we obtained have a high genetic diversity. DL1, DL2, MS3 and MS5 may be some new types of ADV because of so obvious differences compared with foreign isolates in the genes sequences.展开更多
试验旨在研究猪骨形态发生蛋白-6(bone morphogenetic protein 6,BMP-6)基因多态性及其与产仔性能的关联。采用PCR-SSCP技术检测了野猪、民猪、长白猪、北京黑猪、法系大白猪5个猪种268个个体BMP-6基因的多态性,并分析了BMP-6基因多态...试验旨在研究猪骨形态发生蛋白-6(bone morphogenetic protein 6,BMP-6)基因多态性及其与产仔性能的关联。采用PCR-SSCP技术检测了野猪、民猪、长白猪、北京黑猪、法系大白猪5个猪种268个个体BMP-6基因的多态性,并分析了BMP-6基因多态性与法系大白猪产仔性能的关联性。结果表明,猪BMP-6基因CDS区1104位存在1个G→A的同义突变,产生AA、AB、BB 3种基因型,其中在野猪和民猪中,A等位基因频率高;在长白猪、北京黑猪和法系大白猪中,B等位基因频率高。不同基因型对法系大白猪产仔数和产活仔数的影响差异显著(P<0.05),初步认为BMP-6基因多态性与产仔性能显著关联。展开更多
[目的]研究禽6型副粘病毒生物学特性。[方法]从来自吉林省自然保护区的野鸭拭子中分离出1株禽6型副粘病毒(Avian paramyxovirus type 6,APMV6),进行了形态学、血清学、生物学和病毒分子生物学鉴定,测定该病毒全基因组序列,并对F和HN毒...[目的]研究禽6型副粘病毒生物学特性。[方法]从来自吉林省自然保护区的野鸭拭子中分离出1株禽6型副粘病毒(Avian paramyxovirus type 6,APMV6),进行了形态学、血清学、生物学和病毒分子生物学鉴定,测定该病毒全基因组序列,并对F和HN毒力基因进行进化分析。[结果]成功分离鉴定出1株APMV6,命名为JL-127,基因组全长为16 236 nt,毒力基因进化结果表明,JL-127株与TW及FE株遗传关系较近,与HK株遗传关系较远。[结论]首次对我国野鸭源APMV6分离株进行全基因组测序,并对其进行遗传进化分析。展开更多
Molecular genetic maps were commonly constructed by analyzing the segregation of restriction fragment length polymorphisms (RFLPs). Here we described methodology-marker sequences in a new mapping based on recent docum...Molecular genetic maps were commonly constructed by analyzing the segregation of restriction fragment length polymorphisms (RFLPs). Here we described methodology-marker sequences in a new mapping based on recent documents. With the methods they were unique sequences detected by the polymerase chain reaction (PCR). Each of the methods had its Iimitations and the current trend was to integrate the maps produced by the different methods. Marker sequences contained mainly expressed sequence tags (ESTs),polymorphie sequence-tagged sites (STSs), randomly amplified polymorphic DNA (RAPDs), cIeaved amplified polymorphic sequences (CAPS), amplified fragment Iength pofymorphism (AFLPs), genorne sequence sampling (GSS) and sequence-tagged connectors (STCs) in this paper.展开更多
Growth hormone gene (GH) ofRhinopithecus roxellanae was amplified by PCR based on the sequences of the reported mammalian growth hormone gene for the first time. The amplified fragment was about 1.8 kb. It was cloned ...Growth hormone gene (GH) ofRhinopithecus roxellanae was amplified by PCR based on the sequences of the reported mammalian growth hormone gene for the first time. The amplified fragment was about 1.8 kb. It was cloned and its upper stream was sequenced. This sequencing region consists of a 5′flanking regulatory region, exon I and part of exon II, intron I of growth hormone gene. Comparing the corresponding sequences of growth hormone gene betweenRhinopithecus roxellanae and the porcine, we concluded that the homology reached 81% in the region, and there was high conservation in the 5′flanking sequence. The kinds of amino acids of exon I and exon II for about 90% were the same to those in pig. Many mutations occurred in the degenerate site of the triplet code. In the nucleotides of intron I, there were only 72% homologies with those in pig. It means that introns and 3′flanking sequence maybe play an important part in growth hormone gene regulation of the different animals.展开更多
文摘The samples of minks from several main mink farming areas were detected by the methods of counter immunoelectrophoresis(CIEP)and polymerase chain reaction (PCR)and some data about Aleutian mink disease parvovirus(ADV)infection in farmed mink was obtained. Partial VP2 genes,which include a hypervariable region, of 16 ADV strains isolated were separately amplified by PCR and sequenced. Comparison and analysis of these genes sequences and the related sequences of ADV-G,ADV-Utah1 and ADV-K that reported in the USA and European had been completed. The result shows that : firstly, the closed relationship exists between domestic ADV isolates and foreign ones,and the domestic AD maybe introduced from foreign countries. Secondly, the ADV isolates we obtained have a high genetic diversity. DL1, DL2, MS3 and MS5 may be some new types of ADV because of so obvious differences compared with foreign isolates in the genes sequences.
文摘试验旨在研究猪骨形态发生蛋白-6(bone morphogenetic protein 6,BMP-6)基因多态性及其与产仔性能的关联。采用PCR-SSCP技术检测了野猪、民猪、长白猪、北京黑猪、法系大白猪5个猪种268个个体BMP-6基因的多态性,并分析了BMP-6基因多态性与法系大白猪产仔性能的关联性。结果表明,猪BMP-6基因CDS区1104位存在1个G→A的同义突变,产生AA、AB、BB 3种基因型,其中在野猪和民猪中,A等位基因频率高;在长白猪、北京黑猪和法系大白猪中,B等位基因频率高。不同基因型对法系大白猪产仔数和产活仔数的影响差异显著(P<0.05),初步认为BMP-6基因多态性与产仔性能显著关联。
文摘Molecular genetic maps were commonly constructed by analyzing the segregation of restriction fragment length polymorphisms (RFLPs). Here we described methodology-marker sequences in a new mapping based on recent documents. With the methods they were unique sequences detected by the polymerase chain reaction (PCR). Each of the methods had its Iimitations and the current trend was to integrate the maps produced by the different methods. Marker sequences contained mainly expressed sequence tags (ESTs),polymorphie sequence-tagged sites (STSs), randomly amplified polymorphic DNA (RAPDs), cIeaved amplified polymorphic sequences (CAPS), amplified fragment Iength pofymorphism (AFLPs), genorne sequence sampling (GSS) and sequence-tagged connectors (STCs) in this paper.
文摘Growth hormone gene (GH) ofRhinopithecus roxellanae was amplified by PCR based on the sequences of the reported mammalian growth hormone gene for the first time. The amplified fragment was about 1.8 kb. It was cloned and its upper stream was sequenced. This sequencing region consists of a 5′flanking regulatory region, exon I and part of exon II, intron I of growth hormone gene. Comparing the corresponding sequences of growth hormone gene betweenRhinopithecus roxellanae and the porcine, we concluded that the homology reached 81% in the region, and there was high conservation in the 5′flanking sequence. The kinds of amino acids of exon I and exon II for about 90% were the same to those in pig. Many mutations occurred in the degenerate site of the triplet code. In the nucleotides of intron I, there were only 72% homologies with those in pig. It means that introns and 3′flanking sequence maybe play an important part in growth hormone gene regulation of the different animals.
