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黑豆多酚氧化酶的分离纯化及其酶学特性研究 被引量:5
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作者 张宽朝 全明吉 《中国饲料》 北大核心 2012年第19期38-41,共4页
试验用磷酸缓冲液提取黑豆粉中的多酚氧化酶,经30%~80%饱和硫酸铵分级沉淀、透析脱盐、DE-52阴离子交换层析对黑豆多酚氧化酶进行分离纯化。酶学特性研究结果表明,在30 min以内,酶活与反应时间基本呈线性关系。在磷酸盐缓冲液中黑豆多... 试验用磷酸缓冲液提取黑豆粉中的多酚氧化酶,经30%~80%饱和硫酸铵分级沉淀、透析脱盐、DE-52阴离子交换层析对黑豆多酚氧化酶进行分离纯化。酶学特性研究结果表明,在30 min以内,酶活与反应时间基本呈线性关系。在磷酸盐缓冲液中黑豆多酚氧化酶的最适pH为6.8,最适反应温度为50℃,最适底物浓度为0.1%。在供试浓度范围内,高浓度Cu2+、Mg2+、Zn2+、Fe2+、Na+、Ca2+及抗坏血酸、EDTA、Na2SO3均是黑豆多酚氧化酶的抑制剂。 展开更多
关键词 黑豆 多酚氧化酶 纯化 酶学特性
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Collective Bands in Neutron-Rich ^(104)Mo Nucleus
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作者 YANG Li-Ming ZHU Sheng-Jiang +24 位作者 LI Ke J.H.Hamilton A.V.Ramayya J.K.Hwang X.Q.Zhang ZHU Ling-Yan GAN Cui-Yun M.Sakhaee LONG Gui-Lu XU Rui-Qing HANG Zheng JIANG Zhuo JON Myong-Gil W.C.Ma B.R.S.Babu J.Komicki E.F.Jones J.D.Cole R.Aryaeinejad M.W.Drigert I.Y.Lee J.O.Rasmussen M.A.Stoyer M.Ter-Akopian A.V.Daniel 《Chinese Physics Letters》 SCIE CAS CSCD 2001年第1期24-26,共3页
Levels in the neutron-rich ^(104)Mo nucleus have been investigated by observing promptγ-rays from the spontaneous fission of 252Cf with the Gammasphere detector array.The ground-state band,the one-phonon and the two-... Levels in the neutron-rich ^(104)Mo nucleus have been investigated by observing promptγ-rays from the spontaneous fission of 252Cf with the Gammasphere detector array.The ground-state band,the one-phonon and the two-phononγ-vibrational bands as well as a quasiparticle band have been confirmed and expanded with spin up to 14ħ.Other two side bands probably built on new quasiparticle states are identified.The possible configurations for the quasiparticle bands are discussed.Two of the quasiparticle bands show larger moments of inertia and may have pair-free characteristics.The levels of the ground-state band,the one-phononγ-band and the two-phononγ-band calculated from a general collective model are in close agreement with the experimental data. 展开更多
关键词 BANDS PHONON QUASI
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Observation of a Collective Oblate Band in ^(137)Ce
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作者 ZHU Sheng-Jiang ZHU Ling-Yan +10 位作者 LI Ming GAN Cui-Yun MSakhaee YANG Li-Ming ZHANG Zheng JIANG Zhuo LONG Gui-Lu Jon Myang-Gil WEN Shu-Xian WU Xiao-Guang LIU Xiang-An 《Chinese Physics Letters》 SCIE CAS CSCD 2000年第7期480-482,共3页
High spin states in ^(137) Ce have been studied by using the heavy-ion induced reaction ^(124)Sn(^(18) O, 5n) carried out at China Institute of Atomic Energy. Partial level scheme with spin up to (43/2^(-)), including... High spin states in ^(137) Ce have been studied by using the heavy-ion induced reaction ^(124)Sn(^(18) O, 5n) carried out at China Institute of Atomic Energy. Partial level scheme with spin up to (43/2^(-)), including a high spin collective rotational band observed for the first time, has been established. The collective band shows the properties similar to those oblate bands with γ ~-60° in neighboring nuclei and probably originates from the five quasi-particle configuration νs_(1/2)■ [vh_(11/2)]^(2)π9_(7/2)■ πh_(11/2). The characteristics of this oblate band have been discussed. 展开更多
关键词 COLLECTIVE neighboring BANDS
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