Objective: To investigate the time course of serum S-100 concentrations of patients with acute cerebral infarction,and their relation with the clinical data and the prognosis. Methods: Serum S-100 levels were serially...Objective: To investigate the time course of serum S-100 concentrations of patients with acute cerebral infarction,and their relation with the clinical data and the prognosis. Methods: Serum S-100 levels were serially determined in 36 patients with acute cerebral infarction within 12 h, at 24 h and day 2, 3, 4, 5, 7 and 10 after acute cerebral infarction and in 20 age- and sex-matched control subjects. An S-100 content assay was performed using a two-site radioimmunoassay technique. The clinical status was assessed using NIH Stroke Scale. The functional deficit at 4 weeks after acute cerebral infarction was scored using the modified Rankin scale. A cranial computed tomography was performed initially. Results: Elevated concentrations of S-100 (>0.2 μg/L) were observed in 29 of 36 patients with acute cerebral infarction,but none of the control subjects. The S-100 peak levels were at day 2 and 3 after acute cerebral infarction and were significantly high in those patients with severe neurological deficit at admission, with extensive infarction or with space-occupying effect of ischemic edema as compared with the rest of the populations. Conclusion: Serum S-100 level assay can be used as a peripheral marker of ischemic brain damage, and may be helpful for evaluation of therapeutic effects in acute ischemic stroke.展开更多
Objective To study the features of the activity changes of glutamate (GLu ) in the hippocampusand hypothalamus and its effects on the activation of the hypothalamus-pituitary-adrenal axis (HPA ) duringacute cerebral i...Objective To study the features of the activity changes of glutamate (GLu ) in the hippocampusand hypothalamus and its effects on the activation of the hypothalamus-pituitary-adrenal axis (HPA ) duringacute cerebral ischemia (ACI ). Methods: The changes of Glu content, corticotrophin releasing hormone(CRH ) mRNA expression level and adrenocorticotropic hormone (ACTH ) concentration were determinedwith high-performance liquid chromatography (HPLC ) and in situ hybridization in different time intervals after middle cerebral artery occlusion (MCAO) in rats. Results: Glu content was increased rapidly in the hippocampus and hypothalamus 15 min after MCAO and reached the peak (the average Glu content in the hippocarnpus and hypothalamus were 21. 50± 2. 88 mg/g wt and 14. 20±2. 58 mg/g wt respectively) in the lsthour after MCAO and it returned rapidly to the base line level after reperfusion. The Gln content in the hippocampus and hypothalamus went up once more in the 24th hour of reperfusion, remained at a relatively highlevel till the 48th hour of reperfusion and then declined gradually. The expression level of CRH mRNA wasmarkedly enhanced in the temporal cortex, hippocampus and hypothalamus in the lst hour after MCAO andthis condition was kept on till the 96th hour of reperfusion. In the same time, the plasma level of ACTH wasrelatively increased. In the peak stage of reperfusion injury,there was a positive correlation of the Glu contentin the hypothalamus with the number of positive cells of CRH mRNA expression and the plasma level of ACTH. Conclusion: The central CRH system is possible to locate mainly in the limbic system and Gln might beone of the factors to induce excessive excitable stress response of the HPA axis.展开更多
文摘Objective: To investigate the time course of serum S-100 concentrations of patients with acute cerebral infarction,and their relation with the clinical data and the prognosis. Methods: Serum S-100 levels were serially determined in 36 patients with acute cerebral infarction within 12 h, at 24 h and day 2, 3, 4, 5, 7 and 10 after acute cerebral infarction and in 20 age- and sex-matched control subjects. An S-100 content assay was performed using a two-site radioimmunoassay technique. The clinical status was assessed using NIH Stroke Scale. The functional deficit at 4 weeks after acute cerebral infarction was scored using the modified Rankin scale. A cranial computed tomography was performed initially. Results: Elevated concentrations of S-100 (>0.2 μg/L) were observed in 29 of 36 patients with acute cerebral infarction,but none of the control subjects. The S-100 peak levels were at day 2 and 3 after acute cerebral infarction and were significantly high in those patients with severe neurological deficit at admission, with extensive infarction or with space-occupying effect of ischemic edema as compared with the rest of the populations. Conclusion: Serum S-100 level assay can be used as a peripheral marker of ischemic brain damage, and may be helpful for evaluation of therapeutic effects in acute ischemic stroke.
文摘Objective To study the features of the activity changes of glutamate (GLu ) in the hippocampusand hypothalamus and its effects on the activation of the hypothalamus-pituitary-adrenal axis (HPA ) duringacute cerebral ischemia (ACI ). Methods: The changes of Glu content, corticotrophin releasing hormone(CRH ) mRNA expression level and adrenocorticotropic hormone (ACTH ) concentration were determinedwith high-performance liquid chromatography (HPLC ) and in situ hybridization in different time intervals after middle cerebral artery occlusion (MCAO) in rats. Results: Glu content was increased rapidly in the hippocampus and hypothalamus 15 min after MCAO and reached the peak (the average Glu content in the hippocarnpus and hypothalamus were 21. 50± 2. 88 mg/g wt and 14. 20±2. 58 mg/g wt respectively) in the lsthour after MCAO and it returned rapidly to the base line level after reperfusion. The Gln content in the hippocampus and hypothalamus went up once more in the 24th hour of reperfusion, remained at a relatively highlevel till the 48th hour of reperfusion and then declined gradually. The expression level of CRH mRNA wasmarkedly enhanced in the temporal cortex, hippocampus and hypothalamus in the lst hour after MCAO andthis condition was kept on till the 96th hour of reperfusion. In the same time, the plasma level of ACTH wasrelatively increased. In the peak stage of reperfusion injury,there was a positive correlation of the Glu contentin the hypothalamus with the number of positive cells of CRH mRNA expression and the plasma level of ACTH. Conclusion: The central CRH system is possible to locate mainly in the limbic system and Gln might beone of the factors to induce excessive excitable stress response of the HPA axis.
