建立了Qu ECh ERS前处理结合超高效液相色谱-串联质谱同时检测猪组织中赛庚啶和可乐定残留量的方法。猪肉样品添加0.5 g C18粉,猪肝和猪肾样品添加3.0 g硫酸镁和2.0 g氯化钠,再分别加入10 m L 0.1%甲酸乙腈进行提取,提取液经浓缩后采用L...建立了Qu ECh ERS前处理结合超高效液相色谱-串联质谱同时检测猪组织中赛庚啶和可乐定残留量的方法。猪肉样品添加0.5 g C18粉,猪肝和猪肾样品添加3.0 g硫酸镁和2.0 g氯化钠,再分别加入10 m L 0.1%甲酸乙腈进行提取,提取液经浓缩后采用LC-MS/MS进行检测,内标法定量。该方法线性关系良好,猪肉、猪肝和猪肾中赛庚啶和可乐定在1~50μg/kg浓度范围内加标回收率分别为62%~112%和106%~121%,相对标准偏差均<10%,方法检出限为0.5μg/kg。方法灵敏度高,选择性好,可作为猪组织中赛庚啶和可乐定残留量检测的确证方法。展开更多
The article discusses a method that simultaneously determination of nitrofuran metabolites in chicken muscle by UPLC-MS/MS.The nitrofuran metabolites were derived and extracted from the chicken muscle,and detected by ...The article discusses a method that simultaneously determination of nitrofuran metabolites in chicken muscle by UPLC-MS/MS.The nitrofuran metabolites were derived and extracted from the chicken muscle,and detected by electrospray ionization and in the positive ion mode.It has higher sensitivity and shorter analytical time.This method is more suitable for determination of nitrofuran metabolites in the chicken muscle.展开更多
A UPLC-MS/MS method was established for the determination of fifteen kinds of β-agonists in feed.The analytes were separated on a column of BEH C18 100 mm×2.1 mm(i.d.) employing methanol as mobile phase A and 0....A UPLC-MS/MS method was established for the determination of fifteen kinds of β-agonists in feed.The analytes were separated on a column of BEH C18 100 mm×2.1 mm(i.d.) employing methanol as mobile phase A and 0.2% formic acid as mobile phase B(VA∶VB=1∶1).The analytes were determined by tandem MS under ESI+ and MRM mode.The linearity of fifteen kinds of β-agonists was range of 1-50 μg/L.The spiked recoveries of fifteen kinds of β-agonists ranged from 60% to 105%.The LOD and LOQ of the method were 0.1 mg/kg and 0.05 mg/kg,respectively.展开更多
文摘建立了Qu ECh ERS前处理结合超高效液相色谱-串联质谱同时检测猪组织中赛庚啶和可乐定残留量的方法。猪肉样品添加0.5 g C18粉,猪肝和猪肾样品添加3.0 g硫酸镁和2.0 g氯化钠,再分别加入10 m L 0.1%甲酸乙腈进行提取,提取液经浓缩后采用LC-MS/MS进行检测,内标法定量。该方法线性关系良好,猪肉、猪肝和猪肾中赛庚啶和可乐定在1~50μg/kg浓度范围内加标回收率分别为62%~112%和106%~121%,相对标准偏差均<10%,方法检出限为0.5μg/kg。方法灵敏度高,选择性好,可作为猪组织中赛庚啶和可乐定残留量检测的确证方法。
文摘The article discusses a method that simultaneously determination of nitrofuran metabolites in chicken muscle by UPLC-MS/MS.The nitrofuran metabolites were derived and extracted from the chicken muscle,and detected by electrospray ionization and in the positive ion mode.It has higher sensitivity and shorter analytical time.This method is more suitable for determination of nitrofuran metabolites in the chicken muscle.
文摘A UPLC-MS/MS method was established for the determination of fifteen kinds of β-agonists in feed.The analytes were separated on a column of BEH C18 100 mm×2.1 mm(i.d.) employing methanol as mobile phase A and 0.2% formic acid as mobile phase B(VA∶VB=1∶1).The analytes were determined by tandem MS under ESI+ and MRM mode.The linearity of fifteen kinds of β-agonists was range of 1-50 μg/L.The spiked recoveries of fifteen kinds of β-agonists ranged from 60% to 105%.The LOD and LOQ of the method were 0.1 mg/kg and 0.05 mg/kg,respectively.
