The mutagenic effects of discharge plasma and plasma+PAW(plasma and its activated water combined) on Astragalus adsurgens Pall seeds were explored. Needle array-plate dielectric-barrier discharge plasma was used to tr...The mutagenic effects of discharge plasma and plasma+PAW(plasma and its activated water combined) on Astragalus adsurgens Pall seeds were explored. Needle array-plate dielectric-barrier discharge plasma was used to treat A. adsurgens Pall seeds and PAW was prepared at the same time.The deionized water and the obtained PAW were used to cultivate plasma-treated seeds in groups and the survival rate of each group was counted. Results showed that the survival rate of the treated seeds of A. adsurgens Pall when cultured with deionized water was not significantly different from that of the control check(CK). The culture with PAW had an obvious lethal effect and each group reached the half-lethal dose. Reactive oxygen species(ROS) and RNA-Seq analysis of seedlings in the 3 h treatment group showed that the content of ROS in the 3 d post-treatment group was significantly higher than that of the CK. Because expression of the gene with the function of scavenging superoxide free radicals was upregulated, the ROS content of seedlings on the sixth day was significantly lower than that on the third day. Plasma and plasma+PAW treatments changed a large number of gene expressions;particularly, the plasma+PAW group caused plant-growth genes to be significantly upregulated. After treatment, the seedlings of A. adsurgens Pall may grow faster and have higher nutritional value. This research is of great significance to the wider application of isoelectronic bodies and their activated water physicochemical mutagens in biological effects and breeding research.展开更多
We put forward a new method for measuring the entropy production in the living cell.It involves heating the sample by alternating the electric field and recording the outward heat flow.The entropy production in a norm...We put forward a new method for measuring the entropy production in the living cell.It involves heating the sample by alternating the electric field and recording the outward heat flow.The entropy production in a normal cell MCF10A and a cancerous cell MDA-MB-231 were measured and compared.The results show that the method is effective for the entropy measurement of a living organism.The scaled electro-induced entropy production rate(SEEP)of MDA-MB-231 monotonically increases with the electric field strength at 5–40 V/cm.While that of MCF10A changes non-monotonically and there exists a peak at 5–30 V/cm.The electro-induced entropy production ratio(EEPR)is smaller than 1 in a large range of field strengths,from 5 to 25 V/cm,which reveals that under 5–25 V/cm electric field exposure,the direction of the entropy flow may be changed from normal tissue to cancerous cells.We present a facile and effective strategy for experimentally investigating the thermodynamic properties of the cell and give a deeper insight into the physical difference between normal and cancerous cells under electric field exposure.展开更多
基金supported by National Natural Science Foundation of China (Nos. 51767020 and 52067017)the Inner Mongolia Autonomous Region Science and Technology Plan Project (No. 2020GG0280)the Natural Science Foundation of Inner Mongolia Autonomous Region (Nos. 2019MS06025 and 2020MS01016)。
文摘The mutagenic effects of discharge plasma and plasma+PAW(plasma and its activated water combined) on Astragalus adsurgens Pall seeds were explored. Needle array-plate dielectric-barrier discharge plasma was used to treat A. adsurgens Pall seeds and PAW was prepared at the same time.The deionized water and the obtained PAW were used to cultivate plasma-treated seeds in groups and the survival rate of each group was counted. Results showed that the survival rate of the treated seeds of A. adsurgens Pall when cultured with deionized water was not significantly different from that of the control check(CK). The culture with PAW had an obvious lethal effect and each group reached the half-lethal dose. Reactive oxygen species(ROS) and RNA-Seq analysis of seedlings in the 3 h treatment group showed that the content of ROS in the 3 d post-treatment group was significantly higher than that of the CK. Because expression of the gene with the function of scavenging superoxide free radicals was upregulated, the ROS content of seedlings on the sixth day was significantly lower than that on the third day. Plasma and plasma+PAW treatments changed a large number of gene expressions;particularly, the plasma+PAW group caused plant-growth genes to be significantly upregulated. After treatment, the seedlings of A. adsurgens Pall may grow faster and have higher nutritional value. This research is of great significance to the wider application of isoelectronic bodies and their activated water physicochemical mutagens in biological effects and breeding research.
基金Supported by the National Natural Science Foundation of China(Nos 90403010 and 60963015)Distinguished Scientist Award 2008 of Inner Mongolia Autonomous Region and Inner Mongolia Autonomous Region Natural Science Foundation(No 2009BS0102).
文摘We put forward a new method for measuring the entropy production in the living cell.It involves heating the sample by alternating the electric field and recording the outward heat flow.The entropy production in a normal cell MCF10A and a cancerous cell MDA-MB-231 were measured and compared.The results show that the method is effective for the entropy measurement of a living organism.The scaled electro-induced entropy production rate(SEEP)of MDA-MB-231 monotonically increases with the electric field strength at 5–40 V/cm.While that of MCF10A changes non-monotonically and there exists a peak at 5–30 V/cm.The electro-induced entropy production ratio(EEPR)is smaller than 1 in a large range of field strengths,from 5 to 25 V/cm,which reveals that under 5–25 V/cm electric field exposure,the direction of the entropy flow may be changed from normal tissue to cancerous cells.We present a facile and effective strategy for experimentally investigating the thermodynamic properties of the cell and give a deeper insight into the physical difference between normal and cancerous cells under electric field exposure.
