Lymphocyte chalone was partially purified by ultrafiltration and chromatography onSep hadex G75 column.Its inhibitory effect on proliferation of T,B lymphoctes was demonstratedby[~3H]-TdR incorporation method.In mouse...Lymphocyte chalone was partially purified by ultrafiltration and chromatography onSep hadex G75 column.Its inhibitory effect on proliferation of T,B lymphoctes was demonstratedby[~3H]-TdR incorporation method.In mouse bone marrow transplantation model,the treatmentwith lymphocyte chalone in vivo and in vitro was found to have significantly improved the 30 daysurvival rate and mean survival time,suggesting its inhibitory effect on acute graft-versus-host dis-ease(GVHD)and the helpful effect on H-2 incompatible tone marrow transplantation in mice.展开更多
A crude preparation of lymphocyte chalone was extracted from calf Spleen using coldaoctone and distilled water.Its inhibitory cffect on mouse lymphocyte proliferation was demonstratedby <sup>3</sup>H-TdR i...A crude preparation of lymphocyte chalone was extracted from calf Spleen using coldaoctone and distilled water.Its inhibitory cffect on mouse lymphocyte proliferation was demonstratedby <sup>3</sup>H-TdR incorporation method in vitro.This extract shoed no inhibitory effect on the<sup>3</sup>H-TdR uptake of K<sub>5</sub>62 cells and displayed no cytotoxicity to mouse lymphocyte,suggesting thatthere existed the activity of lymphocytc chalone in the extract.The crude chalone inhibited <sup>3</sup>H-TdRuptake of mouse lymphocyte stimulated with concanavalin A（Con A）and mixed lymphocytc re-sponse（MLR）,and the degree of the inhibition was greater as the doses of the extract in culturesbecame higher,indicating it had inhibitory effect on T lymphocytes Its influence on the numbers ofrosette-forming cells（RFC）and plaque-forming cells（PFC）in the spleen of mice revealed that ithad inhibitory effocts on some stages of immunization,including the actions on T and B展开更多
Using a flow cytometry (FCM), we detected and enumerated the positive cell percentage of T cell receptor (TCRα/β), CD3, CD18, and CD25, in normal human peripheral blood mononuclear cells (PBMC) irradiated by γ ray ...Using a flow cytometry (FCM), we detected and enumerated the positive cell percentage of T cell receptor (TCRα/β), CD3, CD18, and CD25, in normal human peripheral blood mononuclear cells (PBMC) irradiated by γ ray in vitro and PBMC from persons who suffered acute radiation symptoms 3 years earlier. The results showed that after PBMC from the normal persons were irradiated with 1～6 Gy dose the positive cell percentage of TCR, CD3, CD18, and CD25, did not change significantly when analysis was conducted immediately after PBMC were irradiated, while the positive cell percentage of TCR, CD3 and CD25, decreased with the increase in irradiation dose and increased with prolongation of culture time significantly at different levels. The change in positive cell percentage of CD18 was relatively smaller than that of the others after irradiated PBMC were cultured in vitro for 8～14 d. Immunocytochemical analysis showed that more TCR and CD3 positive stains presented in cytoplasm 8 ～12d after irradiation. These results implied that the decrement of positive TCR and CD3 T cells induced by irradiation might be related to abnormal expression and disabled assembly of TCR/CD3 complex.展开更多
The susceptibility was compared between murine bone marr ow hemopoietic cells and splenic lymphocytes to four major factors of cryopreservation process: toxicity of dimethyl sulfoxide (DMSO), cooling rate,Tris-NH4Cl t...The susceptibility was compared between murine bone marr ow hemopoietic cells and splenic lymphocytes to four major factors of cryopreservation process: toxicity of dimethyl sulfoxide (DMSO), cooling rate,Tris-NH4Cl treatment, and dilution after thawing. When the concentration of DMSO was over 20%, the proliferative function or viability of both kinds of cells decreased markedly. Injury to hemopoietic stem cells and lymphoeytes was found more severe in rapid cooling than in slow cooling, and the intensity of iujury seemed alike under the same fast cooling condition. Pretreatment of bone marrow cells with Tris-NH4Cl did not significantly accentuate the injury of frozen-thawed hemopoietic stem cells. Dilution of the frozen-thawed cells in ice bath or at a fast rate was harmful to both cells. No significant differential susceptibilities of the two kinds of cells to each factor were found. These results indicate that selective destruction or inactivation of lymphocytes by cold treatment among bone marrow cells may be impossible.展开更多
基金This projection was supported by the National Natural Science Fundation of China
文摘Lymphocyte chalone was partially purified by ultrafiltration and chromatography onSep hadex G75 column.Its inhibitory effect on proliferation of T,B lymphoctes was demonstratedby[~3H]-TdR incorporation method.In mouse bone marrow transplantation model,the treatmentwith lymphocyte chalone in vivo and in vitro was found to have significantly improved the 30 daysurvival rate and mean survival time,suggesting its inhibitory effect on acute graft-versus-host dis-ease(GVHD)and the helpful effect on H-2 incompatible tone marrow transplantation in mice.
文摘A crude preparation of lymphocyte chalone was extracted from calf Spleen using coldaoctone and distilled water.Its inhibitory cffect on mouse lymphocyte proliferation was demonstratedby <sup>3</sup>H-TdR incorporation method in vitro.This extract shoed no inhibitory effect on the<sup>3</sup>H-TdR uptake of K<sub>5</sub>62 cells and displayed no cytotoxicity to mouse lymphocyte,suggesting thatthere existed the activity of lymphocytc chalone in the extract.The crude chalone inhibited <sup>3</sup>H-TdRuptake of mouse lymphocyte stimulated with concanavalin A（Con A）and mixed lymphocytc re-sponse（MLR）,and the degree of the inhibition was greater as the doses of the extract in culturesbecame higher,indicating it had inhibitory effect on T lymphocytes Its influence on the numbers ofrosette-forming cells（RFC）and plaque-forming cells（PFC）in the spleen of mice revealed that ithad inhibitory effocts on some stages of immunization,including the actions on T and B
文摘Using a flow cytometry (FCM), we detected and enumerated the positive cell percentage of T cell receptor (TCRα/β), CD3, CD18, and CD25, in normal human peripheral blood mononuclear cells (PBMC) irradiated by γ ray in vitro and PBMC from persons who suffered acute radiation symptoms 3 years earlier. The results showed that after PBMC from the normal persons were irradiated with 1～6 Gy dose the positive cell percentage of TCR, CD3, CD18, and CD25, did not change significantly when analysis was conducted immediately after PBMC were irradiated, while the positive cell percentage of TCR, CD3 and CD25, decreased with the increase in irradiation dose and increased with prolongation of culture time significantly at different levels. The change in positive cell percentage of CD18 was relatively smaller than that of the others after irradiated PBMC were cultured in vitro for 8～14 d. Immunocytochemical analysis showed that more TCR and CD3 positive stains presented in cytoplasm 8 ～12d after irradiation. These results implied that the decrement of positive TCR and CD3 T cells induced by irradiation might be related to abnormal expression and disabled assembly of TCR/CD3 complex.
文摘The susceptibility was compared between murine bone marr ow hemopoietic cells and splenic lymphocytes to four major factors of cryopreservation process: toxicity of dimethyl sulfoxide (DMSO), cooling rate,Tris-NH4Cl treatment, and dilution after thawing. When the concentration of DMSO was over 20%, the proliferative function or viability of both kinds of cells decreased markedly. Injury to hemopoietic stem cells and lymphoeytes was found more severe in rapid cooling than in slow cooling, and the intensity of iujury seemed alike under the same fast cooling condition. Pretreatment of bone marrow cells with Tris-NH4Cl did not significantly accentuate the injury of frozen-thawed hemopoietic stem cells. Dilution of the frozen-thawed cells in ice bath or at a fast rate was harmful to both cells. No significant differential susceptibilities of the two kinds of cells to each factor were found. These results indicate that selective destruction or inactivation of lymphocytes by cold treatment among bone marrow cells may be impossible.
