Inhibin was purified from swine ovarian fluid by deposition and column deposition.The purified inhibin was diluted with ureasodiumacetate,and then was mixed with Freund adjuvant with same volume.Each of the twenty...Inhibin was purified from swine ovarian fluid by deposition and column deposition.The purified inhibin was diluted with ureasodiumacetate,and then was mixed with Freund adjuvant with same volume.Each of the twenty goats was given subcutaneous injections of the purified inhibin 2 mg at neck.The goats were given boosterinjections of the inhibin 1 mg that was mixed with ureasodiumacetate and Freund incomplete adjuvant after 30 and 50 days.Five goats in control group were given admixture of urea and sodium acetate and adjuvant at the same time.The goats were injected with prostaglandin F2a 005 mg at 21 d and 31 d after the last immunization.The blood samples were collected at 30 d,51 d and 60 d after the first immunization and before immunization.The plasma FSH concentrates was assayed with RIA and the inhibin antibody titer was assayed with agar plate diffusion method.The plasma antibody titer was 1∶16256 at 60 d after first immunization.The plasma FSH concentrates of the immunized goats was higher than those of the control group(202 vs.12 mIU/ml,PZ<005).The number of follicle and the number of corpora lutea of the immunized group were as twice as those of the control(1931 vs.1223 and 225 vs.110,respectively).It indicated that inhibin purified from swine ovarian fluid could induce goat produce high ovulation rate and it can improve reproductive traits of goat.展开更多
In order to amplify the partial bcl-2 gene of goose,we designed a pair of primers according to the reported chicken bcl-2 gene sequence in the GenBank,and obtained the gene by reverse transcript-polymerase chain react...In order to amplify the partial bcl-2 gene of goose,we designed a pair of primers according to the reported chicken bcl-2 gene sequence in the GenBank,and obtained the gene by reverse transcript-polymerase chain reaction(RT-PCR).Then the gene was cloned into pMD-18T vector and identified by PCR.The nucleotide and amino acid sequences of goose partial bcl-2 gene were compared with the counterpart sequences of chicken,and the nucleotide homology was 92.49%.This is the first report of bcl-2 partial nucleotide sequence of goose.展开更多
文摘Inhibin was purified from swine ovarian fluid by deposition and column deposition.The purified inhibin was diluted with ureasodiumacetate,and then was mixed with Freund adjuvant with same volume.Each of the twenty goats was given subcutaneous injections of the purified inhibin 2 mg at neck.The goats were given boosterinjections of the inhibin 1 mg that was mixed with ureasodiumacetate and Freund incomplete adjuvant after 30 and 50 days.Five goats in control group were given admixture of urea and sodium acetate and adjuvant at the same time.The goats were injected with prostaglandin F2a 005 mg at 21 d and 31 d after the last immunization.The blood samples were collected at 30 d,51 d and 60 d after the first immunization and before immunization.The plasma FSH concentrates was assayed with RIA and the inhibin antibody titer was assayed with agar plate diffusion method.The plasma antibody titer was 1∶16256 at 60 d after first immunization.The plasma FSH concentrates of the immunized goats was higher than those of the control group(202 vs.12 mIU/ml,PZ<005).The number of follicle and the number of corpora lutea of the immunized group were as twice as those of the control(1931 vs.1223 and 225 vs.110,respectively).It indicated that inhibin purified from swine ovarian fluid could induce goat produce high ovulation rate and it can improve reproductive traits of goat.
文摘In order to amplify the partial bcl-2 gene of goose,we designed a pair of primers according to the reported chicken bcl-2 gene sequence in the GenBank,and obtained the gene by reverse transcript-polymerase chain reaction(RT-PCR).Then the gene was cloned into pMD-18T vector and identified by PCR.The nucleotide and amino acid sequences of goose partial bcl-2 gene were compared with the counterpart sequences of chicken,and the nucleotide homology was 92.49%.This is the first report of bcl-2 partial nucleotide sequence of goose.
