The experiment was conducted to study the effects and possible mechanism of GLP-2 on proliferation,metabolism and apoptosis of cultured enterocytes from a 28-d weaned piglet injured by exposure to β-conglycinin.A cel...The experiment was conducted to study the effects and possible mechanism of GLP-2 on proliferation,metabolism and apoptosis of cultured enterocytes from a 28-d weaned piglet injured by exposure to β-conglycinin.A cell damage model was established to investigate cell proliferation, metabolism and apoptosis by exposing primary cell cultures of intestinal epithelial cells(IEC) to 1.2 and 2.4 mg/mL β-conglycinin.A 2×3 factorial experiment was then used to study the effect of different GLP-2 concentrations of(1×10<sup>-9</sup>,1×10<sup>-8</sup> and 1×10<sup>-7</sup>mol/L),in combination with the two concentrations ofβ-conglycinin.Cells exposed to the allergenβ-conglycinin had decreased(P【0.05) MTT OD;decreased (P【0.01) protein retention and total protein content of cells;increased(P【0.01) LDH and caspase-3 activities and decreased(P【0.05) Na<sup>+</sup>,K<sup>+</sup>-ATPase activity.When GLP-2 was used in combination withβ-conglycinin,MTT OD,protein retention,total protein content and Na<sup>+</sup>,K<sup>+</sup>-ATPase activity significantly increased(P【0.05);LDH activity gradually decreased(P【0.05 or P【0.01) and Caspase-3 activity significantly decreased(P【0.01) with increasing concentrations of GLP-2.The results indicated thatβ-conglycinin had adverse effects on proliferation and integrity of IEC in vitro.GLP-2 relieved or prevented the adverse effects ofβ-conglycinin on proliferation and integrity of IEC by regulating Na<sup>+</sup>,K<sup>+</sup>- ATPase and Caspase-3 activities,and consequently affecting cell metabolism.展开更多
The purpose of this study was to evaluate the effect and the potential mechanism of administering a pGRF gene plasmid on the growth and immunological function of weanling piglets subjected to immune-stress.Eighteen we...The purpose of this study was to evaluate the effect and the potential mechanism of administering a pGRF gene plasmid on the growth and immunological function of weanling piglets subjected to immune-stress.Eighteen weanling(Duroc×Landrace×Large White) piglets aged 35 d±2 d and initial BW of 7.86 kg±0.59 kg were randomly assigned to three treatments according to gender and BW by using a single factor design.The three treatments were injections of a pGRF gene plasmid,pGRF gene plasmid followed by challenge with lipopolysaccharide(LPS),and LPS to piglets not receiving the plasmid.Each treatment group consisted of six piglets.The results were as follows:piglets in the pGRF gene plasmid plus LPS treatment had a better growth performance than those only receiving LPS(P【0.05), and F/G of piglets in the pGRF gene plasmid plus LPS group were very slightly lower(P】0.05) than those in the LPS group;serum levels of IGF-1 in the pGRF gene plasmid plus LPS group were significantly higher than those in the LPS group(P【0.05 or P【0.01);serum levels of IgG in the pGRF gene plasmid plus LPS group were higher than those in the LPS group(P【0.05);serum levels of IL-1 and IL-6 in the pGRF gene plasmid plus LPS group were significantly lower than those in the LPS group(P【0.05 or P【0.01).展开更多
为探究色氨酸对断奶仔猪生长性能的影响,本次试验对相关方面的研究进行了Meta分析。通过计算机检索中国知网、维普、万方、Web of science、scopus、Pubmed、EBSCO、Embase、Cochrance数据库,收集了色氨酸对仔猪影响的试验研究,按照纳...为探究色氨酸对断奶仔猪生长性能的影响,本次试验对相关方面的研究进行了Meta分析。通过计算机检索中国知网、维普、万方、Web of science、scopus、Pubmed、EBSCO、Embase、Cochrance数据库,收集了色氨酸对仔猪影响的试验研究,按照纳入标准和排除标准筛选文献和提取数据。总计收集了871篇文献,筛选排除后纳入近20年文献共11篇,通过Review Manager 5.3软件进行Meta分析,使用随机效应模型计算日增重、采食量和血清尿素氮的标准化均数差(SMD)及95%置信区间(CI),使用固定效应模型计算肝脏增重的标准化均数差(SMD)及95%置信区间(CI),通过亚组分析和敏感性分析解释异质性来源,利用漏斗图评估发表偏倚。结果表明:饲料中添加色氨酸极显著提高了断奶仔猪的平均日增重(P<0.01)和平均日采食量(P<0.01);饲料中添加色氨酸极显著降低了断奶仔猪血清尿素氮浓度(P<0.01);饲料中添加色氨酸提高了断奶仔猪肝脏增重(P=0.21)。亚组分析显示,色氨酸浓度≥0.25%能极显著提高断奶仔猪平均日增重(P=0.004,SMD=40.10,95%CI:13.12~67.08)、平均日采食量(P<0.01,SMD=39.46,95%CI:19.63~59.29);断奶日龄≥28 d能极显著提高断奶仔猪平均日增重(P=0.001,SMD=25.27,95%CI:9.90~40.63)。综上所述,在饲料中添加色氨酸能够很好的提高断奶仔猪的平均日增重、平均日采食量和肝脏增重,降低仔猪血清尿素氮浓度。当断奶日龄≥28 d时,平均日增重效果更好;当色氨酸浓度≥0.25%时,对断奶仔猪平均日增重及平均日采食量的提高更为显著。展开更多
基金supported by Program for Changjiang Scholars and Innovative Reseach Team in University (IRTO 555)Applied Basic Research(045Y029-031) of Sichuan Province,People's Republic of China
文摘The experiment was conducted to study the effects and possible mechanism of GLP-2 on proliferation,metabolism and apoptosis of cultured enterocytes from a 28-d weaned piglet injured by exposure to β-conglycinin.A cell damage model was established to investigate cell proliferation, metabolism and apoptosis by exposing primary cell cultures of intestinal epithelial cells(IEC) to 1.2 and 2.4 mg/mL β-conglycinin.A 2×3 factorial experiment was then used to study the effect of different GLP-2 concentrations of(1×10<sup>-9</sup>,1×10<sup>-8</sup> and 1×10<sup>-7</sup>mol/L),in combination with the two concentrations ofβ-conglycinin.Cells exposed to the allergenβ-conglycinin had decreased(P【0.05) MTT OD;decreased (P【0.01) protein retention and total protein content of cells;increased(P【0.01) LDH and caspase-3 activities and decreased(P【0.05) Na<sup>+</sup>,K<sup>+</sup>-ATPase activity.When GLP-2 was used in combination withβ-conglycinin,MTT OD,protein retention,total protein content and Na<sup>+</sup>,K<sup>+</sup>-ATPase activity significantly increased(P【0.05);LDH activity gradually decreased(P【0.05 or P【0.01) and Caspase-3 activity significantly decreased(P【0.01) with increasing concentrations of GLP-2.The results indicated thatβ-conglycinin had adverse effects on proliferation and integrity of IEC in vitro.GLP-2 relieved or prevented the adverse effects ofβ-conglycinin on proliferation and integrity of IEC by regulating Na<sup>+</sup>,K<sup>+</sup>- ATPase and Caspase-3 activities,and consequently affecting cell metabolism.
文摘The purpose of this study was to evaluate the effect and the potential mechanism of administering a pGRF gene plasmid on the growth and immunological function of weanling piglets subjected to immune-stress.Eighteen weanling(Duroc×Landrace×Large White) piglets aged 35 d±2 d and initial BW of 7.86 kg±0.59 kg were randomly assigned to three treatments according to gender and BW by using a single factor design.The three treatments were injections of a pGRF gene plasmid,pGRF gene plasmid followed by challenge with lipopolysaccharide(LPS),and LPS to piglets not receiving the plasmid.Each treatment group consisted of six piglets.The results were as follows:piglets in the pGRF gene plasmid plus LPS treatment had a better growth performance than those only receiving LPS(P【0.05), and F/G of piglets in the pGRF gene plasmid plus LPS group were very slightly lower(P】0.05) than those in the LPS group;serum levels of IGF-1 in the pGRF gene plasmid plus LPS group were significantly higher than those in the LPS group(P【0.05 or P【0.01);serum levels of IgG in the pGRF gene plasmid plus LPS group were higher than those in the LPS group(P【0.05);serum levels of IL-1 and IL-6 in the pGRF gene plasmid plus LPS group were significantly lower than those in the LPS group(P【0.05 or P【0.01).
