In order to survey the infectious situation of canine coronavirus (CCV) in giant panda population, a virus neutralization test detecting specific antibodies against CCV in giant panda抯 sera was established by using t...In order to survey the infectious situation of canine coronavirus (CCV) in giant panda population, a virus neutralization test detecting specific antibodies against CCV in giant panda抯 sera was established by using two-fold dilutions of serum and 100 TCID50 of the virus. The 62 sera samples of giant pandas, which were gathered from zoos and reserve region of Sichuan Province, China were detected. The neutralization antibody titer of 1:4 was recognized as the positive criterion, 8 sera samples were detected to be positive, and the positive rate was 12.9%. The titers of neutralizing antibody ranged from 1:8 to 1:32. It was the first comprehensive investigation on neutralization antibodies against CCV in giant panda population in China. The results of study showed that the infection of CCV in giant panda population was universal, which has posed a threat to the health of giant panda. Therefore, it is incumbent on us to study safe and effective vaccines to protect giant panda against CCV infection.展开更多
This study aimed to investigate the eff ect of LyPB on the intestinal microfl ora of giant pandas with indigestion,using high-throughput sequencing(HTS)technology.The species distribution and microfl oral density and ...This study aimed to investigate the eff ect of LyPB on the intestinal microfl ora of giant pandas with indigestion,using high-throughput sequencing(HTS)technology.The species distribution and microfl oral density and diversity before and after administration of the LyPB probiotic agent were analyzed.LyPB evidently has the ability to adjust the fl oral imbalance in the panda’s intestine.To test the eff ects of LyPB on the microfl ora of the panda gut,fecal samples were taken from a healthy giant panda(Anan)without administration of LyPB and from a dyspeptic giant panda Yangyang before and after LyPB administration.Compared with the sample obtained from healthy Anan(anan-c)and that obtained from dyspeptic Yangyang before LyPB administration(yangyang1),the sample taken from Yangyang(yangyang2)after LyPB administration displayed a signifi cant increase in the operational taxonomic unit index.An increase in the Chao index indicated an increase in the microfl oral richness,while an increase in the Shannon index indicated an increase in microfl oral diversity.At phylum and genus levels,a signifi cant increase was observed in the density of probiotic bacteria of phylum fi rmicutes,genus Streptococcus,while a drastic reduction in the density of Escherichia coli/Escherichia coli Shigella/bacteria of genus Shigella was observed.Data obtained in this study shows that LyPB preparations successfully improve the microbial structure within the panda’s intestinal canal by signifi cantly increasing the eff ective microbial community and decreasing the number of pathogenic microbes.展开更多
Arundinaria spanostachya is the main bamboo species on which giant pandas forage.Information on its regeneration capacity to meet the foraging demands of pandas is important to ensure the long-term survival of pandas....Arundinaria spanostachya is the main bamboo species on which giant pandas forage.Information on its regeneration capacity to meet the foraging demands of pandas is important to ensure the long-term survival of pandas.In 2014,2 plot types(grazed versus control)were selected in the winter habitat used by one wild giant panda.Various morphological and regeneration characteristics of the bamboo were assessed in the respective plots during 2014 and 2015.The panda avoided feeding on bamboo with a basal diameter of<6 mm,preferentially feeding on intermediate-sized bamboo.The density of new shoots and the recruitment rate of shoots were significantly higher in the grazed plots compared to the control plots.The annual recruitment rate of shoots was markedly higher than the annual mortality rate in the same plot type.After grazing,the basal diameter and height of new bamboo were similar between the 2 plots.In the grazed plots,the basal diameter of new bamboo was similar to that of the stumps.Innutrition and foraging by insects were the main factors influencing the survival of new shoots.Our results showed that grazing by giant pandas helped the clonal regeneration of A.spanostachya populations,with the basal diameter of new bamboo shoots meeting the foraging demand of pandas.However,information on the carrying capacity of A.spanostachya is necessary to ensure sufficient forage matter is available for reintroduced giant pandas.Furthermore,fertilization and insect control measures should be implemented during the shooting period to optimize A.spanostachya resources.展开更多
The endangered giant panda (Ailuropoda melanoleuca) is endemic to th e mountains of Sichuan, Gansu and Shaanxi Provinces in China. The species had nu mbered over 2000 animals in early 1970s, but declined to no more th...The endangered giant panda (Ailuropoda melanoleuca) is endemic to th e mountains of Sichuan, Gansu and Shaanxi Provinces in China. The species had nu mbered over 2000 animals in early 1970s, but declined to no more than 1,000 anim als fragmented into perhaps 32 subpopulations in late 1990s as the result of num erous detrimental forces such as habitat shrinking, poaching and bamboo flowerin g. The mass flowering and followed die-off of bamboo played key roles in the dec lination of panda population in the past three decades. It trigged the starvatio n and following high mortality of giant pandas in 1970s over Min Mountains and i n 1980s across Qionglai Mountains in Sichuan Province. The situation of survivor ship is made worse by the fact that the panda habitat is fragmented into many sm all 'islands', each containing only a few pandas. Such small, isolated panda pop ulations are rendered even more vulnerable to extinction form threats such as ha bitat degradation, natural disaster, disease, and the deleterious effects of inb reeding. So restoring the panda habitat and reintroduction pandas to their histo rical habitat might be an important issue for protecting the giant panda in wild .展开更多
基金This research was supported by National Science Founda-tion of China (No. 30000123) and Conversation Department of Wildlife Ani-mal & Plants of State Forestry Bureau.
文摘In order to survey the infectious situation of canine coronavirus (CCV) in giant panda population, a virus neutralization test detecting specific antibodies against CCV in giant panda抯 sera was established by using two-fold dilutions of serum and 100 TCID50 of the virus. The 62 sera samples of giant pandas, which were gathered from zoos and reserve region of Sichuan Province, China were detected. The neutralization antibody titer of 1:4 was recognized as the positive criterion, 8 sera samples were detected to be positive, and the positive rate was 12.9%. The titers of neutralizing antibody ranged from 1:8 to 1:32. It was the first comprehensive investigation on neutralization antibodies against CCV in giant panda population in China. The results of study showed that the infection of CCV in giant panda population was universal, which has posed a threat to the health of giant panda. Therefore, it is incumbent on us to study safe and effective vaccines to protect giant panda against CCV infection.
文摘This study aimed to investigate the eff ect of LyPB on the intestinal microfl ora of giant pandas with indigestion,using high-throughput sequencing(HTS)technology.The species distribution and microfl oral density and diversity before and after administration of the LyPB probiotic agent were analyzed.LyPB evidently has the ability to adjust the fl oral imbalance in the panda’s intestine.To test the eff ects of LyPB on the microfl ora of the panda gut,fecal samples were taken from a healthy giant panda(Anan)without administration of LyPB and from a dyspeptic giant panda Yangyang before and after LyPB administration.Compared with the sample obtained from healthy Anan(anan-c)and that obtained from dyspeptic Yangyang before LyPB administration(yangyang1),the sample taken from Yangyang(yangyang2)after LyPB administration displayed a signifi cant increase in the operational taxonomic unit index.An increase in the Chao index indicated an increase in the microfl oral richness,while an increase in the Shannon index indicated an increase in microfl oral diversity.At phylum and genus levels,a signifi cant increase was observed in the density of probiotic bacteria of phylum fi rmicutes,genus Streptococcus,while a drastic reduction in the density of Escherichia coli/Escherichia coli Shigella/bacteria of genus Shigella was observed.Data obtained in this study shows that LyPB preparations successfully improve the microbial structure within the panda’s intestinal canal by signifi cantly increasing the eff ective microbial community and decreasing the number of pathogenic microbes.
基金supported by National Natural science Foundation of China(No.31370367)
文摘Arundinaria spanostachya is the main bamboo species on which giant pandas forage.Information on its regeneration capacity to meet the foraging demands of pandas is important to ensure the long-term survival of pandas.In 2014,2 plot types(grazed versus control)were selected in the winter habitat used by one wild giant panda.Various morphological and regeneration characteristics of the bamboo were assessed in the respective plots during 2014 and 2015.The panda avoided feeding on bamboo with a basal diameter of<6 mm,preferentially feeding on intermediate-sized bamboo.The density of new shoots and the recruitment rate of shoots were significantly higher in the grazed plots compared to the control plots.The annual recruitment rate of shoots was markedly higher than the annual mortality rate in the same plot type.After grazing,the basal diameter and height of new bamboo were similar between the 2 plots.In the grazed plots,the basal diameter of new bamboo was similar to that of the stumps.Innutrition and foraging by insects were the main factors influencing the survival of new shoots.Our results showed that grazing by giant pandas helped the clonal regeneration of A.spanostachya populations,with the basal diameter of new bamboo shoots meeting the foraging demand of pandas.However,information on the carrying capacity of A.spanostachya is necessary to ensure sufficient forage matter is available for reintroduced giant pandas.Furthermore,fertilization and insect control measures should be implemented during the shooting period to optimize A.spanostachya resources.
文摘The endangered giant panda (Ailuropoda melanoleuca) is endemic to th e mountains of Sichuan, Gansu and Shaanxi Provinces in China. The species had nu mbered over 2000 animals in early 1970s, but declined to no more than 1,000 anim als fragmented into perhaps 32 subpopulations in late 1990s as the result of num erous detrimental forces such as habitat shrinking, poaching and bamboo flowerin g. The mass flowering and followed die-off of bamboo played key roles in the dec lination of panda population in the past three decades. It trigged the starvatio n and following high mortality of giant pandas in 1970s over Min Mountains and i n 1980s across Qionglai Mountains in Sichuan Province. The situation of survivor ship is made worse by the fact that the panda habitat is fragmented into many sm all 'islands', each containing only a few pandas. Such small, isolated panda pop ulations are rendered even more vulnerable to extinction form threats such as ha bitat degradation, natural disaster, disease, and the deleterious effects of inb reeding. So restoring the panda habitat and reintroduction pandas to their histo rical habitat might be an important issue for protecting the giant panda in wild .
