目的通过文献计量学定量分析胎儿α-地中海贫血的热点和前沿趋势。方法采用VOSviewer和CiteSpace 6.3.1软件对Web of Science核心合集数据库、中国知网和万方数据知识服务平台2009年1月至2023年12月关于胎儿α-地中海贫血和重型α-地中...目的通过文献计量学定量分析胎儿α-地中海贫血的热点和前沿趋势。方法采用VOSviewer和CiteSpace 6.3.1软件对Web of Science核心合集数据库、中国知网和万方数据知识服务平台2009年1月至2023年12月关于胎儿α-地中海贫血和重型α-地中海贫血的文献进行年发文量、作者、期刊、参考文献及关键词可视化分析。结果共纳入964篇中文文献和1130篇英文文献。英文发文量在2016年前呈上升趋势,2018年发文量达到顶峰,中文文献发文量于2015年最高。发文量最多的国家是美国,泰国清迈大学发文量最多。Tongsong T是英文文献发文量最高的作者,杜丽是中文文献发文量最多的作者。Hemoglobin是发文量最多的英文期刊(87篇),中国优生与遗传杂志是中文发文量最多的期刊。共被引文献聚类排名前3位的是基因编辑、多态性、羟基脲。关键词分析产前诊断、基因型、基因诊断和治疗策略为该领域的高频词汇,基因治疗可能是未来的研究焦点。结论该领域的研究主要集中在产前诊断和治疗策略方面。无创产前诊断和治疗方法的进展将改变目前胎儿重型α-地中海贫血的管理方式。展开更多
目的探讨胎儿超声心动图对矫正性大动脉转位(corrected transposition of the great arteries,cTGA)的诊断价值,分析漏、误诊的原因,以提高cTGA产前诊断的准确性。方法选取13例经产前超声心动图证实为cTGA胎儿的超声资料,超声检查的共...目的探讨胎儿超声心动图对矫正性大动脉转位(corrected transposition of the great arteries,cTGA)的诊断价值,分析漏、误诊的原因,以提高cTGA产前诊断的准确性。方法选取13例经产前超声心动图证实为cTGA胎儿的超声资料,超声检查的共同征象是房室连接不一致、心室大动脉连接不一致。结果13例cTGA胎儿中超声诊断正确11例(84.62%),误诊2例(15.38%),其中1例误诊为完全性大动脉转位、室间隔缺损,1例误诊为右位心、右心室双出口、室间隔缺损。13例中心房正位、心室左襟、主动脉位于肺动脉的左前(SLL)型12例,心房反位、心室右襟、主动脉位于肺动脉的右前(IDD)型1例;1例(7.69%)为单纯性,12例(92.31%)合并其他心血管畸形,包括室间隔缺损12例、三尖瓣反流8例、中位心7例、肺动脉狭窄4例、房室传导阻滞2例、Ebstein畸形1例。结论胎儿超声心动图可以比较准确地诊断cTGA,但容易误诊。展开更多
BACKGROUND: Premature rupture of membrane(PROM) is linked to significant maternal prenatal mortalities and morbidity. In Ethiopia, where maternal mortality is still high, the maternal and fetal outcomes in PROM is ver...BACKGROUND: Premature rupture of membrane(PROM) is linked to significant maternal prenatal mortalities and morbidity. In Ethiopia, where maternal mortality is still high, the maternal and fetal outcomes in PROM is very important to decrease maternal and child mortality and for better management and prevention of complications. Thus, this study aimed to detect the maternal and fetal outcomes and associated factors in term PROM at Mizan-Aman General Hospital, south-west Ethiopia.METHODS: A retrospective cross sectional study was conducted using data available at MizanAman General Hospital during a period of 3 years(January 2011 to December 2013). We examined records of 4 525 women who gave birth in the hospital; out of these women, 185 were diagnosed with term PROM and all of them were included in the study. The data of these women were collected using a checklist based on registration books. The data were analyzed using SPSS version 20.0 statistical package. The association between independent and dependent variables was assessed by bivariate and multiple logistic regression analyses. 95%CI and P value less than 0.05 were considered statistically signifi cant.RESULTS: Of the 4 525 women who gave birth in the hospital, 202 were complicated by term PROM. About 22.2% of the women showed unfavorable maternal outcomes. The most common cause of maternal morbidity and mortality was puerperal sepsis. About 33.5% of neonates experienced unfavorable outcomes. The duration of PROM >12 hours(AOR=5.6, 95%CI 1.3–24.1) latency >24 hours(AOR=2.8, 95%CI 1.7–11.8), residing in rural areas(AOR=4.2, 95%CI 3.96–29.4) and birth weight less than 2 500 g were associated with unfavorable outcomes.CONCLUSION: Women residing in rural areas, long latency, and neonates with birth weight less 2 500 g may have unfavorable outcomes. Therefore, optimum obstetric and medical care is essential for the reduction of the devastating complications related to disorders.展开更多
Objective: To explore the molecular regulation mechanism of carvedilol in attenuating the reversion back towards fetal energy metabolism during the development of cardiac hypertrophy induced by coarctation of abdomina...Objective: To explore the molecular regulation mechanism of carvedilol in attenuating the reversion back towards fetal energy metabolism during the development of cardiac hypertrophy induced by coarctation of abdominal aorta (CAA) in male Wistar rats. Methods: Hemodynamic and ventricular remodeling parameters, free fatty acid content in the serum were measured in the experimental animals at 16 weeks after the surgical CAA, the rats receiving carvedilol intervention (CAR) after CAA, and those with sham operation (SH). The expressions of muscle carnitine palmitoyltransferaseⅠ (M-CPTⅠ) and medium chain acyl-CoA dehydrogenase (MCAD) mRNA in the cardiac myocytes from every group were studied with RT-PCR. Results: Significant left ventricular hypertrophy were observed in the rats 16 weeks after coarctation operation (P<0.05), together with significant free fatty acids accumulation and downregulation of M-CPTⅠ and MCAD mRNA (P<0.05) in CAA group. Carvedilol at a dose of 30 mg/kg/d for 12 weeks inhibited the left ventricular hypertrophy induced by pressure overload and enhanced the gene expressions of rate-limiting enzyme (M-CPTⅠ) and key enzyme of fatty acid (MCAD) in the CAR group compared with CAA group (P<0.05). Conclusion: Pressure overload-induced hypertrophy in CAA rats causes the reversion back towards fetal enery metabolism, that is, downregulates the expressions of rate-limiting enzyme and key enzyme of fatty acid oxidation. The intervention therapy with carvedilol, a vasodilating alpha- and beta-adrenoreceptor antagonist, attenuates the reversion of the metabolic gene expression to fetal type through upregulating M-CPTⅠ and MCAD mRNA expressions. Thus, carvedilol may exert cardioprotective effects on heart failure by the mechanism of preserving the adult metabolic gene regulation.展开更多
Human fetal liver cells were cultured in vitro for 12h and the supernatant(Fetal liver cell conditioned medium,FLCM)was collected.The effects of FLCM ongranulopoiesis were studied.The results show that when combined w...Human fetal liver cells were cultured in vitro for 12h and the supernatant(Fetal liver cell conditioned medium,FLCM)was collected.The effects of FLCM ongranulopoiesis were studied.The results show that when combined with colonystimulating factor(CSF),FLCM could significantly stimulate the proliferation of normalmyctoid progenitor cells(CFU-e),and increase ~3H-TdR incorporation into bone mar-row cells.The data suggest that FLCM contains a CSF synergistic activity.展开更多
On the basis of the characteristics of Y-chromosome sequence which consists of Y specific repeat DNA family(DYZI) of 800￣5 000 copies,a pair of primers Y3,Y4 is designed to amplify the specific DNA segment of 446 bp ...On the basis of the characteristics of Y-chromosome sequence which consists of Y specific repeat DNA family(DYZI) of 800￣5 000 copies,a pair of primers Y3,Y4 is designed to amplify the specific DNA segment of 446 bp by the polymerase chain reaction(PCR),so as to detect the presence of Y chromosome in male fetal cell in maternal peripheral blood for the purpose of prenatal determination of fetal sex.The authors made use of PCR amplification of crude DNA from maternal peripheral blood in early, mid,and late pregnancies to determine the fetal sex.Comparison of the results with those of PCR of their corresponding chorionic villi and amniotic fluid,and with the sex of the aborted fetus or the new born showed coincident rates of 93%,100%,and 87. 5% in early, mid,and late pregnancies respectively.The procedure offers a new alternative way for non-invasive prenatal diagnosis,展开更多
A series of experiments are conducted to confirm whether the vectors calculated for an early section of a continuous non-invasive fetal electrocardiogram （fECG） recording can be directly applied to subsequent sectio...A series of experiments are conducted to confirm whether the vectors calculated for an early section of a continuous non-invasive fetal electrocardiogram （fECG） recording can be directly applied to subsequent sections in order to reduce the computation required for real-time monitoring. Our results suggest that it is generally feasible to apply the initial optimal maternal and fetal ECG combination vectors to extract the fECG and maternal ECG in subsequent recorded sections.展开更多
Objective To explore fetal arrhythmia clinical significance and its correlation with fetal prognosis. Methods Twenty-six cases of fetal arrhythmia detected among 12 799 pregnant women recorded over a ten-year period i...Objective To explore fetal arrhythmia clinical significance and its correlation with fetal prognosis. Methods Twenty-six cases of fetal arrhythmia detected among 12 799 pregnant women recorded over a ten-year period in Peking Uinon Medical College (PUMC) Hospital were reviewed retrospectively. Fetal arrhythmia was diagnosed by fetal auscultation, ultrasonography, electric fetal heart monitoring, and fetal echocardiography. Results Twenty-six fetuses were documented with fetal arrhythmia (3 tachycardia, 4 bradycardia, 19 normal heart rate with irregular fetal cardiac rhythm). The incidence of fetal arrhythmia in our hospital was 0.2%. They were diagnosed at the average of 35 weeks’ gestation (15 to 41 weeks). Twenty-two cases were diagnosed by antenatal fetal auscultation, 1 case was diagnosed by ultrasonography, and 3 cases were diagnosed by electric fetal heart monitoring. Fetal echocardiograms were per-formed on 17 fetuses, 6 cases (35.3%) of which showed that ventricular premature beats with normal structure of fetal heart. All neonates survived postnatally and 24 of them (92.3%) were followed up. Echocardiograms were performed for 16 neonates and 2 of them were identified as atrial septal defects with normal heart rhythms. The results of follow-up showed that the two patients had no apparent clinical manifestation. The echocardiogram showed that atrial septal defect obliterated already. Conclusion The prognosis is well for most of the fetuses with arrhythmias, with low incidence of heart deformation.展开更多
文摘目的通过文献计量学定量分析胎儿α-地中海贫血的热点和前沿趋势。方法采用VOSviewer和CiteSpace 6.3.1软件对Web of Science核心合集数据库、中国知网和万方数据知识服务平台2009年1月至2023年12月关于胎儿α-地中海贫血和重型α-地中海贫血的文献进行年发文量、作者、期刊、参考文献及关键词可视化分析。结果共纳入964篇中文文献和1130篇英文文献。英文发文量在2016年前呈上升趋势,2018年发文量达到顶峰,中文文献发文量于2015年最高。发文量最多的国家是美国,泰国清迈大学发文量最多。Tongsong T是英文文献发文量最高的作者,杜丽是中文文献发文量最多的作者。Hemoglobin是发文量最多的英文期刊(87篇),中国优生与遗传杂志是中文发文量最多的期刊。共被引文献聚类排名前3位的是基因编辑、多态性、羟基脲。关键词分析产前诊断、基因型、基因诊断和治疗策略为该领域的高频词汇,基因治疗可能是未来的研究焦点。结论该领域的研究主要集中在产前诊断和治疗策略方面。无创产前诊断和治疗方法的进展将改变目前胎儿重型α-地中海贫血的管理方式。
文摘目的探讨胎儿超声心动图对矫正性大动脉转位(corrected transposition of the great arteries,cTGA)的诊断价值,分析漏、误诊的原因,以提高cTGA产前诊断的准确性。方法选取13例经产前超声心动图证实为cTGA胎儿的超声资料,超声检查的共同征象是房室连接不一致、心室大动脉连接不一致。结果13例cTGA胎儿中超声诊断正确11例(84.62%),误诊2例(15.38%),其中1例误诊为完全性大动脉转位、室间隔缺损,1例误诊为右位心、右心室双出口、室间隔缺损。13例中心房正位、心室左襟、主动脉位于肺动脉的左前(SLL)型12例,心房反位、心室右襟、主动脉位于肺动脉的右前(IDD)型1例;1例(7.69%)为单纯性,12例(92.31%)合并其他心血管畸形,包括室间隔缺损12例、三尖瓣反流8例、中位心7例、肺动脉狭窄4例、房室传导阻滞2例、Ebstein畸形1例。结论胎儿超声心动图可以比较准确地诊断cTGA,但容易误诊。
文摘BACKGROUND: Premature rupture of membrane(PROM) is linked to significant maternal prenatal mortalities and morbidity. In Ethiopia, where maternal mortality is still high, the maternal and fetal outcomes in PROM is very important to decrease maternal and child mortality and for better management and prevention of complications. Thus, this study aimed to detect the maternal and fetal outcomes and associated factors in term PROM at Mizan-Aman General Hospital, south-west Ethiopia.METHODS: A retrospective cross sectional study was conducted using data available at MizanAman General Hospital during a period of 3 years(January 2011 to December 2013). We examined records of 4 525 women who gave birth in the hospital; out of these women, 185 were diagnosed with term PROM and all of them were included in the study. The data of these women were collected using a checklist based on registration books. The data were analyzed using SPSS version 20.0 statistical package. The association between independent and dependent variables was assessed by bivariate and multiple logistic regression analyses. 95%CI and P value less than 0.05 were considered statistically signifi cant.RESULTS: Of the 4 525 women who gave birth in the hospital, 202 were complicated by term PROM. About 22.2% of the women showed unfavorable maternal outcomes. The most common cause of maternal morbidity and mortality was puerperal sepsis. About 33.5% of neonates experienced unfavorable outcomes. The duration of PROM >12 hours(AOR=5.6, 95%CI 1.3–24.1) latency >24 hours(AOR=2.8, 95%CI 1.7–11.8), residing in rural areas(AOR=4.2, 95%CI 3.96–29.4) and birth weight less than 2 500 g were associated with unfavorable outcomes.CONCLUSION: Women residing in rural areas, long latency, and neonates with birth weight less 2 500 g may have unfavorable outcomes. Therefore, optimum obstetric and medical care is essential for the reduction of the devastating complications related to disorders.
文摘Objective: To explore the molecular regulation mechanism of carvedilol in attenuating the reversion back towards fetal energy metabolism during the development of cardiac hypertrophy induced by coarctation of abdominal aorta (CAA) in male Wistar rats. Methods: Hemodynamic and ventricular remodeling parameters, free fatty acid content in the serum were measured in the experimental animals at 16 weeks after the surgical CAA, the rats receiving carvedilol intervention (CAR) after CAA, and those with sham operation (SH). The expressions of muscle carnitine palmitoyltransferaseⅠ (M-CPTⅠ) and medium chain acyl-CoA dehydrogenase (MCAD) mRNA in the cardiac myocytes from every group were studied with RT-PCR. Results: Significant left ventricular hypertrophy were observed in the rats 16 weeks after coarctation operation (P<0.05), together with significant free fatty acids accumulation and downregulation of M-CPTⅠ and MCAD mRNA (P<0.05) in CAA group. Carvedilol at a dose of 30 mg/kg/d for 12 weeks inhibited the left ventricular hypertrophy induced by pressure overload and enhanced the gene expressions of rate-limiting enzyme (M-CPTⅠ) and key enzyme of fatty acid (MCAD) in the CAR group compared with CAA group (P<0.05). Conclusion: Pressure overload-induced hypertrophy in CAA rats causes the reversion back towards fetal enery metabolism, that is, downregulates the expressions of rate-limiting enzyme and key enzyme of fatty acid oxidation. The intervention therapy with carvedilol, a vasodilating alpha- and beta-adrenoreceptor antagonist, attenuates the reversion of the metabolic gene expression to fetal type through upregulating M-CPTⅠ and MCAD mRNA expressions. Thus, carvedilol may exert cardioprotective effects on heart failure by the mechanism of preserving the adult metabolic gene regulation.
文摘Human fetal liver cells were cultured in vitro for 12h and the supernatant(Fetal liver cell conditioned medium,FLCM)was collected.The effects of FLCM ongranulopoiesis were studied.The results show that when combined with colonystimulating factor(CSF),FLCM could significantly stimulate the proliferation of normalmyctoid progenitor cells(CFU-e),and increase ~3H-TdR incorporation into bone mar-row cells.The data suggest that FLCM contains a CSF synergistic activity.
文摘On the basis of the characteristics of Y-chromosome sequence which consists of Y specific repeat DNA family(DYZI) of 800￣5 000 copies,a pair of primers Y3,Y4 is designed to amplify the specific DNA segment of 446 bp by the polymerase chain reaction(PCR),so as to detect the presence of Y chromosome in male fetal cell in maternal peripheral blood for the purpose of prenatal determination of fetal sex.The authors made use of PCR amplification of crude DNA from maternal peripheral blood in early, mid,and late pregnancies to determine the fetal sex.Comparison of the results with those of PCR of their corresponding chorionic villi and amniotic fluid,and with the sex of the aborted fetus or the new born showed coincident rates of 93%,100%,and 87. 5% in early, mid,and late pregnancies respectively.The procedure offers a new alternative way for non-invasive prenatal diagnosis,
基金supported by the National Natural Science Foundation of China(Grant No.61271079)
文摘A series of experiments are conducted to confirm whether the vectors calculated for an early section of a continuous non-invasive fetal electrocardiogram （fECG） recording can be directly applied to subsequent sections in order to reduce the computation required for real-time monitoring. Our results suggest that it is generally feasible to apply the initial optimal maternal and fetal ECG combination vectors to extract the fECG and maternal ECG in subsequent recorded sections.
文摘Objective To explore fetal arrhythmia clinical significance and its correlation with fetal prognosis. Methods Twenty-six cases of fetal arrhythmia detected among 12 799 pregnant women recorded over a ten-year period in Peking Uinon Medical College (PUMC) Hospital were reviewed retrospectively. Fetal arrhythmia was diagnosed by fetal auscultation, ultrasonography, electric fetal heart monitoring, and fetal echocardiography. Results Twenty-six fetuses were documented with fetal arrhythmia (3 tachycardia, 4 bradycardia, 19 normal heart rate with irregular fetal cardiac rhythm). The incidence of fetal arrhythmia in our hospital was 0.2%. They were diagnosed at the average of 35 weeks’ gestation (15 to 41 weeks). Twenty-two cases were diagnosed by antenatal fetal auscultation, 1 case was diagnosed by ultrasonography, and 3 cases were diagnosed by electric fetal heart monitoring. Fetal echocardiograms were per-formed on 17 fetuses, 6 cases (35.3%) of which showed that ventricular premature beats with normal structure of fetal heart. All neonates survived postnatally and 24 of them (92.3%) were followed up. Echocardiograms were performed for 16 neonates and 2 of them were identified as atrial septal defects with normal heart rhythms. The results of follow-up showed that the two patients had no apparent clinical manifestation. The echocardiogram showed that atrial septal defect obliterated already. Conclusion The prognosis is well for most of the fetuses with arrhythmias, with low incidence of heart deformation.
