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Estimating aboveground biomass of Pinus densata-dominated forests using Landsat time series and permanent sample plot data 被引量:10
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作者 Jialong Zhang Chi Lu +1 位作者 Hui Xu Guangxing Wang 《Journal of Forestry Research》 SCIE CAS CSCD 2019年第5期1689-1706,共18页
Southwest China is one of three major forest regions in China and plays an important role in carbon sequestration.Accurate estimations of changes in aboveground biomass are critical for understanding forest carbon cyc... Southwest China is one of three major forest regions in China and plays an important role in carbon sequestration.Accurate estimations of changes in aboveground biomass are critical for understanding forest carbon cycling and promoting climate change mitigation.Southwest China is characterized by complex topographic features and forest canopy structures,complicating methods for mapping aboveground biomass and its dynamics.The integration of continuous Landsat images and national forest inventory data provides an alternative approach to develop a long-term monitoring program of forest aboveground biomass dynamics.This study explores the development of a methodological framework using historical national forest inventory plot data and Landsat TM timeseries images.This method was formulated by comparing two parametric methods:Linear Regression for Multiple Independent Variables(MLR),and Partial Least Square Regression(PLSR);and two nonparametric methods:Random Forest(RF)and Gradient Boost Regression Tree(GBRT)based on the state of forest aboveground biomass and change models.The methodological framework mapped Pinus densata aboveground biomass and its changes over time in Shangri-la,Yunnan,China.Landsat images and national forest inventory data were acquired for 1987,1992,1997,2002 and 2007.The results show that:(1)correlation and homogeneity texture measures were able to characterize forest canopy structures,aboveground biomass and its dynamics;(2)GBRT and RF predicted Pinus densata aboveground biomass and its changes better than PLSR and MLR;(3)GBRT was the most reliable approach in the estimation of aboveground biomass and its changes;and,(4)the aboveground biomass change models showed a promising improvement of prediction accuracy.This study indicates that the combination of GBRT state and change models developed using temporal Landsat and national forest inventory data provides the potential for developing a methodological framework for the long-term mapping and monitoring program of forest aboveground biomass and its changes in Southwest China. 展开更多
关键词 Forest biomass change Gradient Boost Regression Tree LANDSAT MULTI-TEMPORAL images PERMANENT sample PLOTS PINUS densata Shangri-La China
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基于AIS的海洋捕鱼聚集区信息系统设计与开发
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作者 韩暄 侯英姿 +1 位作者 王俊夫 王方雄 《科学技术创新》 2025年第3期61-64,共4页
本文设计并开发了一个基于AIS大数据的海洋捕鱼聚集区信息系统,提供了基于B/S架构的系统界面、服务功能和数据库的设计方案。主要功能包括:利用LSTM+HDBSCAN深度学习算法识别和管理捕鱼聚集区、网络地图浏览、测距、打印输出及用户管理... 本文设计并开发了一个基于AIS大数据的海洋捕鱼聚集区信息系统,提供了基于B/S架构的系统界面、服务功能和数据库的设计方案。主要功能包括:利用LSTM+HDBSCAN深度学习算法识别和管理捕鱼聚集区、网络地图浏览、测距、打印输出及用户管理等服务。系统前端采用Vue实现动态交互界面,后端使用Django处理业务逻辑,深度学习模型使用Python代码实现,数据库采用PostgreSQL进行数据存储和管理。本系统整合了深度学习算法与地理信息系统技术,能够有效地了解渔业资源的分布、变化和利用情况,为海洋空间规划和渔业经济发展提供帮助。 展开更多
关键词 AIS 捕鱼聚集区 WEBGIS LSTM HDBSCAN
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利用核心SNP标记构建豆类蔬菜品种指纹图谱
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作者 吴新义 刘娜 +2 位作者 汪宝根 龚亚明 李国景 《浙江农业科学》 2025年第2期363-369,共7页
豆类蔬菜是我国重要的蔬菜作物。为促进豆类蔬菜品种权保护,以豇豆和菜用豌豆为研究对象,分别利用物种特异的核心单核苷酸多态性(SNP)标记构建了52份豇豆品种(系)和48份豌豆种质的DNA指纹图谱,并对14份豇豆品种(系)的种子纯度进行了检... 豆类蔬菜是我国重要的蔬菜作物。为促进豆类蔬菜品种权保护,以豇豆和菜用豌豆为研究对象,分别利用物种特异的核心单核苷酸多态性(SNP)标记构建了52份豇豆品种(系)和48份豌豆种质的DNA指纹图谱,并对14份豇豆品种(系)的种子纯度进行了检测。该研究结果表明,基于竞争性等位基因特异性PCR(KASP)技术的SNP标记可以用于豆类蔬菜品种的品种权保护、种子真伪区分以及种子纯度鉴定,建议尽快制订并发布基于SNP标记的豆类蔬菜品种鉴定技术标准,为种业和产业健康发展提供技术支撑。 展开更多
关键词 豆类蔬菜 SNP标记 KASP 品种保护 种子纯度
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Drivers of tree regeneration in coniferous monocultures during conversion to mixed forests in Central Europe – Implications for forest restoration management
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作者 Alexander Seliger Christian Ammer +1 位作者 Dominik Seidel Stefan Zerbe 《Forest Ecosystems》 CSCD 2024年第6期910-920,共11页
In Central Europe,anthropogenic coniferous monocultures have been subject to conversion to more diverse mixed forests since the 1990s,however,they are still abundant across many forest landscapes.Artificial and natura... In Central Europe,anthropogenic coniferous monocultures have been subject to conversion to more diverse mixed forests since the 1990s,however,they are still abundant across many forest landscapes.Artificial and natural tree regeneration both play a key role during conversion by determining the species composition and structure of the future forests.Many abiotic and biotic factors can potentially influence the regeneration process and its specific combinations or interactions may be different among tree species and its developmental stages.Here,we aimed to identify and quantify the effect of the most important drivers on the density of the most abundant regenerating tree species(i.e.,Norway spruce and European beech),as well as on species and structural diversity of the tree regeneration.We studied tree regeneration in four former monospecific coniferous stand types(i.e.,Norway spruce,Scots pine,European larch,and Douglas fir)in Southwest Germany that have been under conversion to mixed forests since the 1990s.We sampled tree regeneration in four growth height classes together with a variety of potentially influencing factors on 108 sampling plots and applied multivariate analyses.We identified light availability in the understorey,stand structural attributes,browsing pressure,and diaspore source abundance as the most important factors for the density and diversity of tree regeneration.Particularly,we revealed speciesspecific differences in drivers of regeneration density.While spruce profited from increasing light availability and decreasing stand basal area,beech benefited either from a minor reduction or more strikingly from an increase in overstorey density.Increasing diaspore source abundance positively and a high browsing pressure negatively affected both species equally.Our results suggest that humus and topsoil properties were modified during conversion,probably due to changes in tree species composition and silvicultural activities.The species and structural diversity of the tree regeneration benefitted from increasing light availability,decreasing stand basal area,and a low to moderate browsing pressure.We conclude that forest managers may carefully equilibrate among the regulation of overstorey cover,stand basal area,and browsing pressure to fulfil the objectives of forest conversion,i.e.,establishing and safeguarding a diverse tree regeneration to promote the development of mature mixed forests in the future. 展开更多
关键词 Forest restoration Norway spruce(Picea abies(L.)Karst) European beech(Fagus sylvatica L.) Tree establishment and growth Tree regeneration density Tree species and structural diversity Boruta analysis Generalized additive models(GAMs)
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基于SCARA的柔性葡萄采摘机械臂设计与试验 被引量:1
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作者 杨语录 李长勇 《农机化研究》 北大核心 2025年第1期72-78,86,共8页
针对现今葡萄种植环境狭小且复杂、常见关节型机械臂空间运动灵活度较小和避障能力较弱等问题,为了适应葡萄植株下层枝叶较少、障碍物较少,上层枝叶较多、障碍物较多的特点,设计并开发一款基于SCARA的柔性葡萄采摘机械臂,其底部为SCARA... 针对现今葡萄种植环境狭小且复杂、常见关节型机械臂空间运动灵活度较小和避障能力较弱等问题,为了适应葡萄植株下层枝叶较少、障碍物较少,上层枝叶较多、障碍物较多的特点,设计并开发一款基于SCARA的柔性葡萄采摘机械臂,其底部为SCARA机械臂,上部为柔性机械臂部分;同时,设计了SCARA与柔性机械臂的协调运动控制系统。通过分析机械臂的机械结构和工作原理确定机械臂的末端空间位置和机械臂的运动学关系,利用MatLab绘制其工作空间,并绘制柔性机械臂在随机两个空间点运动的轨迹、速度、加速度图,从而实现SCARA机械臂与柔性机械臂的运动控制。 展开更多
关键词 采摘 柔性机械臂 运动空间 运动学求解 SCARA机械臂 葡萄
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Analysis of the inflection points of height-diameter models
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作者 Tzeng Yih Lam Mark J.Ducey 《Forest Ecosystems》 SCIE CSCD 2024年第4期414-422,共9页
The inflection point is an important feature of sigmoidal height-diameter(H-D)models.It is often cited as one of the properties favoring sigmoidal model forms.However,there are very few studies analyzing the inflectio... The inflection point is an important feature of sigmoidal height-diameter(H-D)models.It is often cited as one of the properties favoring sigmoidal model forms.However,there are very few studies analyzing the inflection points of H-D models.The goals of this study were to theoretically and empirically examine the behaviors of inflection points of six common H-D models with a regional dataset.The six models were the Wykoff(WYK),Schumacher(SCH),Curtis(CUR),HossfeldⅣ(HOS),von Bertalanffy-Richards(VBR),and Gompertz(GPZ)models.The models were first fitted in their base forms with tree species as random effects and were then expanded to include functional traits and spatial distribution.The distributions of the estimated inflection points were similar between the two-parameter models WYK,SCH,and CUR,but were different between the threeparameter models HOS,VBR,and GPZ.GPZ produced some of the largest inflection points.HOS and VBR produced concave H-D curves without inflection points for 12.7%and 39.7%of the tree species.Evergreen species or decreasing shade tolerance resulted in larger inflection points.The trends in the estimated inflection points of HOS and VBR were entirely opposite across the landscape.Furthermore,HOS could produce concave H-D curves for portions of the landscape.Based on the studied behaviors,the choice between two-parameter models may not matter.We recommend comparing seve ral three-parameter model forms for consistency in estimated inflection points before deciding on one.Believing sigmoidal models to have inflection points does not necessarily mean that they will produce fitted curves with one.Our study highlights the need to integrate analysis of inflection points into modeling H-D relationships. 展开更多
关键词 CONCAVITY Forest inventory and analysis Generalized height-diameter models Growth functions Height-diameter functions Mixed-effects modeling Points of inflection Species-specific models
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No generality in biodiversity-productivity relationships along elevation in temperate and subtropical forest landscapes
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作者 Jiayun Zou Yahuang Luo +12 位作者 Rupert Seidl Dominik Thom Jie Liu Lisa Geres Tobias Richter Linjiang Ye Wei Zheng Liangliang Ma Jie Song Kun Xu Dezhu Li Lianming Gao Sebastian Seibold 《Forest Ecosystems》 SCIE CSCD 2024年第3期255-265,共11页
An improved understanding of biodiversity-productivity relationships(BPRs)along environmental gradients is crucial for effective ecosystem management and biodiversity conservation.The stress-gradient hypothesis sugges... An improved understanding of biodiversity-productivity relationships(BPRs)along environmental gradients is crucial for effective ecosystem management and biodiversity conservation.The stress-gradient hypothesis suggests that BPRs are stronger in stressful environments compared to more favorable conditions.However,there is limited knowledge regarding the variation of BPRs along elevational gradients and their generality across different landscapes.To study how BPRs change with elevation,we harnessed inventory data on 6,431 trees from152 plots surveyed twice in eight to ten year intervals in mountain forests of temperate Europe and subtropical Asia.We quantified the relationship between aboveground productivity and different biodiversity measures,including taxonomic,functional,and phylogenetic diversity.To elucidate the processes underlying BPRs,we studied the variation of different functional traits along elevation across landscapes.We found no general pattern of BPRs across landscapes and elevations.Relationships were neutral for all biodiversity measures in temperate forests,and negative for taxonomic and functional diversity in subtropical forests.BPRs were largely congruent between taxonomic,functional and phylogenetic diversity.We found only weak support for the stress-gradient hypothesis,with BPRs turning from negative to positive(effect not significant)close to the tree line in subtropical forests.In temperate forests,however,elevation patterns were strongly modulated by species identity effects as influenced by specific traits.The effect of traits such as community-weighted mean of maximum plant height and wood density on productivity was congruent across landscapes.Our study highlights the context-dependence of BPRs across elevation gradients and landscapes.Species traits are key modulating factors of BPRs and should be considered more explicitly in studies of the functional role of biodiversity.Furthermore,our findings highlight that potential trade-offs between conserving biodiversity and fostering ecosystem productivity exist,which require more attention in policy and management. 展开更多
关键词 Biodiversity-ecosystem functioning Climate mitigation Elevation gradients Forest management Forest productivity Functional traits Nature conservation Phylogenetic diversity
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The effects of data aggregation on long-term projections of forest stands development
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作者 Kobra Maleki Rasmus Astrup +2 位作者 Nicolas Cattaneo Wilson Lara Henao Clara Anton-Fernandez 《Forest Ecosystems》 SCIE CSCD 2024年第3期381-389,共9页
Forest management planning often relies on Airborne Laser Scanning(ALS)-based Forest Management Inventories(FMIs)for sustainable and efficient decision-making.Employing the area-based(ABA)approach,these inventories es... Forest management planning often relies on Airborne Laser Scanning(ALS)-based Forest Management Inventories(FMIs)for sustainable and efficient decision-making.Employing the area-based(ABA)approach,these inventories estimate forest characteristics for grid cell areas(pixels),which are then usually summarized at the stand level.Using the ALS-based high-resolution Norwegian Forest Resource Maps(16 m×16 m pixel resolution)alongside with stand-level growth and yield models,this study explores the impact of three levels of pixel aggregation(standlevel,stand-level with species strata,and pixel-level)on projected stand development.The results indicate significant differences in the projected outputs based on the aggregation level.Notably,the most substantial difference in estimated volume occurred between stand-level and pixel-level aggregation,ranging from-301 to+253 m^(3)·ha^(-1)for single stands.The differences were,on average,higher for broadleaves than for spruce and pine dominated stands,and for mixed stands and stands with higher variability than for pure and homogenous stands.In conclusion,this research underscores the critical role of input data resolution in forest planning and management,emphasizing the need for improved data collection practices to ensure sustainable forest management. 展开更多
关键词 Growth and yield models Dominant species Norway spruce Scots pine BROADLEAVES Forest resource map Stand variability
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Coarse woody debris and wood-colonizing fungi differences between a reserve stand and a managed forest in the Taborz region of Poland 被引量:2
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作者 Slwomir Pieeka Agata Sotnik +1 位作者 Marta Damszel Zbigniew Sierota 《Journal of Forestry Research》 SCIE CAS CSCD 2019年第3期1081-1091,共11页
The aim of this research was to evaluate the amount of woody debris (m3/ha) on the forest floor and the associated wood-colonizing fungi. The study was performed in the Taborz region, known for its Scots pine provenan... The aim of this research was to evaluate the amount of woody debris (m3/ha) on the forest floor and the associated wood-colonizing fungi. The study was performed in the Taborz region, known for its Scots pine provenance experiments, against the background of a recently launched Polish legislation to protect the biodiversity on the forest floor in managed (harvested) stands. We investigated a managed stand (136-years-old) and the reserve stand ‘Sosna Taborska’(261-years-old). In the reserve stand, the mean volume of woody debris was six times higher than in the managed forests, i.e. 65 versus 11 m3/ha. In addition, in the reserve stand, the number of fungi taxa colonizing the dead wood was larger than in the managed stands, with a higher number of fruitbodies. Total fungal richness was higher in the reserve than in the managed stand, i.e. 28 versus 12 species. The dominant taxa at both sites were Fomitopsis pinicola and Fomes The aim of this research was to evaluate the amount of woody debris (m^3/ha) on the forest floor and the associated wood-colonizing fungi. The study was performed in the Taborz region, known for its Scots pine provenance experiments, against the background of a recently launched Polish legislation to protect the biodiversity on the forest floor in managed (harvested) stands. We investigated a managed stand (136-years-old) and the reserve stand ‘Sosna Taborska’(261-years-old). In the reserve stand, the mean volume of woody debris was six times higher than in the managed forests, i.e. 65 versus 11 m^3/ha. In addition, in the reserve stand, the number of fungi taxa colonizing the dead wood was larger than in the managed stands, with a higher number of fruitbodies. Total fungal richness was higher in the reserve than in the managed stand, i.e. 28 versus 12 species. The dominant taxa at both sites were Fomitopsis pinicola and Fomes fomentarius, although some taxa were only found in the reserve (e.g., Stereum hirsutum). The volume of woody debris as well as the diversity of fungi in the managed stand were lower than in the reserve, albeit greater than in other Scots pine stands in Poland. These results testify to the gains in biodiversity yielded by the management conservation management approach at the reserve stand. 展开更多
关键词 Coarse WOODY DEBRIS FUNGI Managed (harvested) STANDS RESERVE Sosna Taborska RESERVE FOREST Indices
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酵母单杂交技术验证叶用莴苣LsHPB互作启动子
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作者 曹佳琰 王惠玉 韩莹琰 《北京农学院学报》 2025年第1期37-43,共7页
【目的】AG和SOC1都属于MADS-box家族基因,AG参与调控多种植物花器官中雄蕊和雌蕊的发育和成熟;SOC1在多种植物中参与整合许多开花途径。以叶用莴苣SOC1-like基因LsHPB基因为研究对象,挖掘出LsHPB基因下游互作蛋白之一LsAG,解析LsHPB参... 【目的】AG和SOC1都属于MADS-box家族基因,AG参与调控多种植物花器官中雄蕊和雌蕊的发育和成熟;SOC1在多种植物中参与整合许多开花途径。以叶用莴苣SOC1-like基因LsHPB基因为研究对象,挖掘出LsHPB基因下游互作蛋白之一LsAG,解析LsHPB参与叶用莴苣抽薹的分子机理,进一步完善MADS-box家族基因调控植物响应高温诱导后抽薹的模型。【方法】通过酵母单杂试验验证转录因子LsHPB与候选基因启动子proLsAG之间的关系,构建双荧光素酶载体,注射烟草并比较检测荧光信号强度和LUC和REN两种酶活性的比值,验证转录因子LsHPB能否激活LsAG启动子。【结果】酵母单杂交试验显示酵母菌在0 ng/mL AbA的SD/-Leu培养基上正常生长,但在含有200 ng/mL AbA抑制浓度的SD/-Leu固体培养基上只有同时含有Prey和Bait的酵母菌株才能生长。随着酵母菌液稀释程度加倍,酵母菌斑的个数明显减少。双荧光素酶试验显示了烟草叶片荧光表达情况,pGreenII62-SK-LsHPB+pGreenII0800-LUC-ProLsAG的组合菌液注射部分荧光信号强度最高,此外此组合LUC和REN两种酶活性的比值高于其他组合。由此可验证LsAG为LsHPB基因下游调控因子,最终确定LsHPB蛋白结合LsAG启动子的互作关系。【结论】初步解析了LsHPB参与叶用莴苣抽薹的分子机理,为完善MADS-box家族基因调控植物响应高温诱导后抽薹的模型奠定基础。 展开更多
关键词 叶用莴苣 酵母单杂 LsAG LsHPB MADS-box家族基因
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基于STM32的小麦籽粒含水率在线检测系统设计
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作者 姬虹 李康 +3 位作者 宋东方 王万章 李保谦 冯伟 《农机化研究》 北大核心 2025年第5期94-101,共8页
为满足联合收获机小麦含水率在线检测需求,设计了一种基于STM32的小麦籽粒含水率在线检测系统。该系统使用STM32F103RCT6嵌入式芯片作为硬件开发平台,采用同侧圆弧电容式含水率检测传感器作为采集终端,经过电路与STM32单片机处理后,将RS... 为满足联合收获机小麦含水率在线检测需求,设计了一种基于STM32的小麦籽粒含水率在线检测系统。该系统使用STM32F103RCT6嵌入式芯片作为硬件开发平台,采用同侧圆弧电容式含水率检测传感器作为采集终端,经过电路与STM32单片机处理后,将RS485采集的数据通过4G发送到云服务器进行数据交互的M2M双向数据传输设备,实现串口设备的无线长距离数据传输,进而实现远程数据通信管理,具有良好的人机交互性。台架验证试验结果表明:性能可靠,检测含水率最大绝对误差为0.87%,最小绝对误差为0.07%,其平均绝对误差为0.29%,相对误差值均小于10%,平均相对误差为4.7%,系统含水率检测值与实际含水率检测值拟合,R^(2)=0.9685能够满足联合收获机谷物含水率在线检测要求。 展开更多
关键词 小麦籽粒 联合收获机 含水率 在线检测 STM32 同侧圆弧电容器
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凡纳对虾parkin共调基因和parkin在抗白斑综合征病毒中的表达分析和SNP开发
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作者 薛倩 李旭鹏 +5 位作者 李洋 栾生 罗坤 孔杰 邢群 孟宪红 《渔业科学进展》 北大核心 2025年第1期194-209,共16页
本实验室前期研究中基于全基因组关联分析(GWAS)方法筛选到抗白斑综合征病毒(WSSV)候选基因:parkin共调基因(PACRG)。PACRG与帕金森病相关基因parkin共用一个双向启动子,二者共同参与细胞自噬过程,从而在细胞保护方面发挥作用。本研究... 本实验室前期研究中基于全基因组关联分析(GWAS)方法筛选到抗白斑综合征病毒(WSSV)候选基因:parkin共调基因(PACRG)。PACRG与帕金森病相关基因parkin共用一个双向启动子,二者共同参与细胞自噬过程,从而在细胞保护方面发挥作用。本研究对凡纳对虾(Penaeus vannamei)PACRG和parkin在抗WSSV中的功能进行探讨,对其mRNA和氨基酸序列进行特征分析,利用real-timePCR技术检测对虾感染WSSV后不同时间、不同组织中PACRG和parkin的表达水平。通过荧光原位杂交技术(FISH)进行空间定位。利用PCR和Sanger测序技术获得单核苷酸多态性位点(SNP)并进行抗WSSV的关联分析。结果显示,PACRG开放阅读框(ORF)全长600bp,编码199个氨基酸,预测包含一个ParcG结构域。parkinORF序列全长1653bp,编码550个氨基酸,预测包含UBQ、IBR结构域和一个信号肽结构。与多物种进行同源序列比对发现,凡纳对虾PACRG氨基酸序列与日本对虾(Penaeus japonicus)的同源性高达89.70%;凡纳对虾parkin氨基酸序列与中国对虾(Penaeus chinensis)和斑节对虾(Penaeus monodon)的同源性高达93.45%。PACRG和parkin蛋白的保守性较高。感染WSSV后,PACRG和parkin在对虾肝胰腺、鳃、肌肉和眼柄中的表达水平发生显著变化,其中,眼柄中PACRG和parkin表现出极相似的组织表达模式。在肌肉中,PACRGmRNA和WSSV在空间位置上呈现高度重叠状态。结合上述结果,推测PACRG和parkin在凡纳对虾与WSSV的互作中发挥功能。在PACRG中筛选到2个SNP位点,在parkin中筛选到15个SNP位点,其中位于parkin非翻译区(UTR)的5个SNP位点与抗WSSV性状显著相关。本文为研究凡纳对虾抗WSSV的分子机制和抗病分子育种提供了理论依据和参考数据。 展开更多
关键词 凡纳对虾 白斑综合征病毒(WSSV) parkin共调基因(PACRG) PARKIN基因 SNP
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Tree structure and diversity shape the biomass of primary temperate mountain forests 被引量:1
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作者 Dheeraj Ralhan Ruffy Rodrigo +14 位作者 Heather Keith Annemiek Irene Stegehuis Jakob Pavlin Yumei Jiang Milos Rydval Juliana Nogueira Alexandre Fruleux Marek Svitok Martin Mikolas Daniel Kozak Martin Dusatko Pavel Janda Oleh Chaskovsky Catalin-Constantin Roibu Miroslav Svoboda 《Forest Ecosystems》 SCIE CSCD 2024年第4期568-579,共12页
Primary forests are spatially diverse terrestrial ecosystems with unique characteristics,being naturally regenerative and heterogeneous,which supports the stability of their carbon storage through the accumulation of ... Primary forests are spatially diverse terrestrial ecosystems with unique characteristics,being naturally regenerative and heterogeneous,which supports the stability of their carbon storage through the accumulation of live and dead biomass.Yet,little is known about the interactions between biomass stocks,tree genus diversity and structure across a temperate montane primary forest.Here,we investigated the relationship between tree structure(variability in basal area and tree size),genus-level diversity(abundance,tree diversity)and biomass stocks in temperate primary mountain forests across Central and Eastern Europe.We used inventory data from726 permanent sample plots from mixed beech and spruce across the Carpathian Mountains.We used nonlinear regression to analyse the spatial variability in forest biomass,structure,and genus-level diversity and how they interact with plot-level tree age,disturbances,temperature and altitude.We found that the combined effects of genus and structural indices were important for addressing the variability in biomass across different spatial scales.Local processes in disturbance regimes and uneven tree age support forest hete rogeneity and the accumulation of live and dead biomass through the natural regeneration,growth and decay of the forest ecosystem.Structural complexities in basal area index,supporte d by genus-level abundance,positively influence total biomass stocks,which was modulated by tree age and disturbances.Spruce forests showed higher tree density and basal area than mixed beech forests,though mixed beech still contributes significantly to biomass across landscapes.Forest heterogeneity was strongly influenced by complexities in forest composition(tree genus diversity,structure).We addressed the importance of primary forests as stable carbon stores,achieved through structure and diversity.Safeguarding such ecosystems is critical for ensuring the stability of the primary forest,carbon store and biodiversity into the future. 展开更多
关键词 Biodiversity indicators Biomass carbon stock Ecosystem functioning Forest composition Primary forest structure Tree size distribution
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TSIT-PatchTST模型:一种净生态系统交换量(NEE)缺失值插补方法
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作者 齐建东 石成城 吴鹏 《北京林业大学学报》 北大核心 2025年第2期105-118,共14页
【目的】净生态系统交换量(NEE)是评估陆地生态系统在全球碳循环中作用的重要指标,NEE原始观测数据缺失值的插补精度会直接影响生态系统关键参数的可靠性和精确性。为提高不同植被NEE在长时间连续性数据缺失情景下的插补精度,提出一种... 【目的】净生态系统交换量(NEE)是评估陆地生态系统在全球碳循环中作用的重要指标,NEE原始观测数据缺失值的插补精度会直接影响生态系统关键参数的可靠性和精确性。为提高不同植被NEE在长时间连续性数据缺失情景下的插补精度,提出一种融合时间序列表征向量的TSIT-PatchTST深度学习模型。【方法】以全球长期通量观测网络站点的碳通量因子数据为研究对象,通过构造短缺失(1 d)、中缺失(7 d)、长缺失(30 d)3种随机连续数据缺失场景,评估边际分布采样法(MDS)、PatchTST模型、TS2Vec-PatchTST模型和TSIT-PatchTST模型在8种不同植被类型下NEE的插补结果。【结果】在短缺失场景下,4种插补方法都表现出最优的性能。随着连续缺失天数的增多,MDS的插补精度逐渐下降,该方法在长缺失场景下已不能对NEE进行有效插补,而其他3种深度学习模型能够有效地插补NEE缺失数据。综合3种缺失场景,TSIT-PatchTST模型表现出最优的插补性能,尤其在长缺失场景下该模型具有较高的插补精度。长缺失场景下,TSIT-PatchTST模型对31个站点插补结果的平均均方误差(MSE)为0.942μmol/(m2·s),平均绝对误差(MAE)为0.628μmol/(m2·s),平均R2为0.457。与PatchTST模型的插补结果相比,TSIT-PatchTST模型平均MSE下降了53.3%,平均MAE下降了39.7%,平均R2相持平。【结论】综合8种植被类型和3种缺失场景的应用结果,得出TSITPatchTST模型的插补效果最佳,并具有适应性。TSIT-PatchTST模型可应用于时间序列数据缺失场景以提高数据插补精度。 展开更多
关键词 深度学习 模型开发 数据插补 TSIT-PatchTST模型 碳循环 植被类型 净生态系统交换量(NEE)
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猪急性腹泻综合征冠状病毒N蛋白间接ELISA抗体检测方法的建立及初步应用
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作者 曾苗苗 杨小曼 +9 位作者 张鑫 刘大凯 时洪艳 张记宇 张燎原 陈建飞 冯廷帅 李修文 石达 冯力 《畜牧兽医学报》 北大核心 2025年第1期319-326,共8页
为建立一种猪急性腹泻综合征冠状病毒(SADS-CoV)血清抗体的快速检测方法,本研究首先经PCR扩增SADS-CoV N基因,纯化鉴定后克隆至表达载体pET-32a(+),构建重组质粒pET-32a-N。重组N蛋白诱导表达并通过SDS-PAGE和Western blot鉴定后,采用... 为建立一种猪急性腹泻综合征冠状病毒(SADS-CoV)血清抗体的快速检测方法,本研究首先经PCR扩增SADS-CoV N基因,纯化鉴定后克隆至表达载体pET-32a(+),构建重组质粒pET-32a-N。重组N蛋白诱导表达并通过SDS-PAGE和Western blot鉴定后,采用纯化的重组N蛋白作为包被抗原,以兔抗猪HRP-IgG作为二抗,利用方阵滴定法优化各反应条件,建立了一种以SADS-CoV N蛋白为靶标的检测SADS-CoV血清抗体的间接ELISA方法,并对此方法的敏感性、特异性、重复性及临床应用价值进行鉴定。SDS-PAGE及Western blot结果显示,重组表达的N蛋白约为70 ku,且具有良好的反应原性。采用该方法检测SADS-CoV阳性血清抗体效价可达1∶3 200,并且与猪流行性腹泻病毒(PEDV)、猪传染性胃肠炎病毒(TGEV)、猪丁型冠状病毒(PDCoV)、猪繁殖与呼吸障碍综合征病毒(PRRSV)、猪轮状病毒(PoRV)和猪圆环病毒(PCV)阳性血清均无交叉反应,具有良好的敏感性和特异性。批内和批间重复性试验的变异系数均小于10%,具有较好的重复性。利用该方法和间接免疫荧光方法(IFA)分别对50份猪临床血清样品进行检测,结果显示二者的总符合率为98%。综上,本研究建立了一种基于SADS-CoV N蛋白的间接ELISA抗体检测方法,可用于临床SADS-CoV血清抗体检测以及流行病学的监测,对防控SADS-CoV的流行具有重要意义。 展开更多
关键词 猪急性腹泻综合征冠状病毒(SADS-CoV) N蛋白 原核表达 间接ELISA
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基于TOPSIS-AISM模型的黄土高原主推玉米品种适应性评价
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作者 李帅 陈亚飞 +6 位作者 王辉 许吉利 王仕稳 孙笑宇 孙秋雨 殷俐娜 邓西平 《干旱地区农业研究》 北大核心 2025年第1期1-12,31,共13页
为构建黄土高原地区玉米品种适应性评价体系,筛选与该地区气候条件相匹配的优良品种,通过2022年和2023年大田试验比较黄土高原50个主推玉米品种在两种降水状况下的产量和穗部性状,基于符合黄土高原气候特征的2023年试验数据,获取了以高... 为构建黄土高原地区玉米品种适应性评价体系,筛选与该地区气候条件相匹配的优良品种,通过2022年和2023年大田试验比较黄土高原50个主推玉米品种在两种降水状况下的产量和穗部性状,基于符合黄土高原气候特征的2023年试验数据,获取了以高产、抗倒伏和高效为目的3大类共11个性状指标,通过Pearson相关性分析判别指标间的相关性,使用CRITIC法确定指标权重,基于TOPSIS-AISM模型得到各品种优劣排序。结果表明,拔节~抽雄期作物需水量和降水量的耦合度较低,导致玉米产量、穗粗、穗重的平均值下降,产量的变异系数增大而百粒重的变异系数减小。TOPSIS-AISM联用筛选出‘陕科6号’、‘登海605’和‘正大12’为稳定较优品种。玉米品种对拔节~抽雄期的降水状况响应敏感,水分利用效率和降水与作物需水耦合系数为高权重指标,可较好地评价黄土高原地区玉米品种适应性。此外,TOPSIS-AISM联用较传统TOPSIS方法更具客观性和普遍性,判定结果排序稳定,可适用于作物品种评价和推广。 展开更多
关键词 玉米品种 适应性评价 对抗结构解释模型(AISM) 逼近理想解排序法(TOPSIS) 黄土高原
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Physiological characteristics changes of Aesculus chinensis seeds during natural dehydration 被引量:8
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作者 喻方圆 杜艳 沈永宝 《Journal of Forestry Research》 SCIE CAS CSCD 2006年第2期103-106,共4页
A study was conducted to determine the physiological characteristics changes of Aesculus chinensis seeds during natural dehydration in 2003. The results showed that A. chinensis seeds were recalcitrant with being high... A study was conducted to determine the physiological characteristics changes of Aesculus chinensis seeds during natural dehydration in 2003. The results showed that A. chinensis seeds were recalcitrant with being highly desiccation-sensitive. The seed moisture content of fresh fruits was higher than 60%. When the seeds were naturally dried for 30 days, their moisture content declined to 30.2% and their viability was completely lost. The seed germination percentage had a small increase at the beginning of desiccation and then decreased rapidly. The relative electrical conductivity of the A. chinensis seeds increased along with a decrease in seed moisture content. However, there was an abnormal increase in relative electrical conductivity when the seed moisture content was between 53.7% and 50.9%. Superoxide dismutase (SOD) activity decreased rapidly in the period of desiccation except for an abnormality when the seed moisture content was between 53.7% and 50.9%. Malondialdehyde (MDA) content increased slowly at the early stage of desiccation and then rose rapidly after the moisture content was below 50.9%. The soluble sugar content in seeds slowly increased with the increasing period of desiccation. The seed germination percentage was at the high level when seed moisture content was in range of 47%- 60%, which suggests that this was the optimum moisture content for maintaining A. chinensis seed viability. 展开更多
关键词 Aesculus chinensis SEEDS Air drying Physiological characteristics Seedmoisture content Seed germination percentage Superoxide dismutase (SOD) activity Malondialdehyde(MDA) content Seed electrical conductivity
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烟草 HSP18.1 基因生信分析及非生物胁迫下表达分析
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作者 刘晨 魏进彬 +6 位作者 宋凯 臧志鹏 高玉珍 杨红静 任维贤 张建栋 王祯 《安徽农业科学》 2025年第1期113-122,共10页
以秦烟1号无菌幼苗为供试材料克隆出HSP18.1基因的CDS区,构建重组质粒,对该基因蛋白进行诱导表达及表达条件优化,通过生物信息学分析,研究其在不同组织中非生物胁迫表达。结果表明:该基因属于HSP20家族,全长615 bp,CDS全长579 bp,编码19... 以秦烟1号无菌幼苗为供试材料克隆出HSP18.1基因的CDS区,构建重组质粒,对该基因蛋白进行诱导表达及表达条件优化,通过生物信息学分析,研究其在不同组织中非生物胁迫表达。结果表明:该基因属于HSP20家族,全长615 bp,CDS全长579 bp,编码192个氨基酸,且含多个磷酸化和糖基化位点,二级结构以无规则卷曲为主;烟草HSP18.1蛋白与拟南芥HSP18.1蛋白同源,且与多个蛋白相互作用。系统发育表明,烟草HSP18.1与文冠果的亲缘关系较近,与小米椒的亲缘关系较远;在热胁迫下NtHSP18.1基因在根、茎叶中被强烈诱导表达,表明NtHSP18.1基因是调节植物耐热性的关键。 展开更多
关键词 烟草 HSP18.1基因 生物信息学 蛋白诱导表达 热胁迫
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基于小流域山坪塘沉积泥沙^(137)Cs的泥沙沉积剖面粒径特征
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作者 成六三 《节水灌溉》 北大核心 2025年第1期96-101,共6页
小流域泥沙来源分析对流域综合治理与水土保持措施评价具有重要意义。选择岩溶区小流域山坪塘为例,从山坪塘沉积泥沙入手,探明山坪塘沉积泥沙剖面与其控制流域内的土地利用类型的^(137)Cs比活度和及黏粒含量的深度分布特征,为反演小流... 小流域泥沙来源分析对流域综合治理与水土保持措施评价具有重要意义。选择岩溶区小流域山坪塘为例,从山坪塘沉积泥沙入手,探明山坪塘沉积泥沙剖面与其控制流域内的土地利用类型的^(137)Cs比活度和及黏粒含量的深度分布特征,为反演小流域土壤侵蚀时空强度分布提供基础。研究结果表明:①山坪塘沉积泥沙的^(137)Cs比活度在垂直剖面上变化较为复杂,最大值出现在山坪塘的底顶部,^(137)Cs比活度范围为0.99~3.58 Bq/kg,平均值为1.8 Bq/kg;②山坪塘控制流域内坡耕地、草地和林地表层土壤的^(137)Cs比活度分布差异性较大,且^(137)Cs比活度极差分别为4.16、2.86和6.36 Bq/kg;③山坪塘旋回沉积泥沙与其控制流域内土地利用类型表层土壤的粒径组分含量主要以黏粒、中粉粒和粉粒为主,且它们的粒径组分含量之间比例基本保持一致;④山坪塘沉积泥沙粒径特征参数(中值粒径、众数粒径、体积加权平均粒径和分形维数D)在垂直剖面上变化基本保持一致,而有效粒径变化较为复杂。研究为开展岩溶小流域山坪塘泥沙沉积来源特征演变提供了重要基础。 展开更多
关键词 岩溶小流域 ^(137)Cs 土壤侵蚀 乌江流域
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Extraction of seed oil from Diospyros lotus optimized using response surface methodology 被引量:5
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作者 Gholamhossein Sodeifian Nedasadat Saadati Ardestani Seyed Ali Sajadian 《Journal of Forestry Research》 SCIE CAS CSCD 2019年第2期709-719,共11页
Oil from seeds of Diospyros lotus was extracted using a conventional method with two different solvents:hexane and petroleum ether. A central composite design with response surface methodology were used to optimize th... Oil from seeds of Diospyros lotus was extracted using a conventional method with two different solvents:hexane and petroleum ether. A central composite design with response surface methodology were used to optimize the process. A second-order polynomial equation was employed, and ANOVA was applied to evaluate the impact of various operating parameters including extraction temperature(x_1; 44.9–70.1 °C), extraction time(x_2;5.0–10.0 h) and solvent to solid ratio(x_3;11.6–28.4 mL g^(-1)), on oil yield. Experiments to validate the model showed decent conformity between predicted and actual values. Extraction conditions for optimal oil yield were 61 °C, 8.75 h extraction duration and 19.25 mL g^(-1) solvent to solid ratio. Under these conditions, the oil yield was predicted to be 5.1340%. Oil samples obtained were then analyzed using gas chromatography. The fatty acid composition revealed the major fatty acids to be oleic acid(C18:1) and linoleic acid(C18:2). The analysis of oil also demonstrated a decent ratio between omega-3 and omega-6 fatty acids. The structure of seeds was imaged using scanning electron microscopy. Oil quality was analyzed thermogravimetrically and by Fourier transform infrared spectroscopy. The assigned nutritional features of the D. lotus oil suggested that it can be used as an edible oil in pharmaceutical and food industry in the future. 展开更多
关键词 DIOSPYROS LOTUS Solvent EXTRACTION PHYSICOCHEMICAL properties FATTY ACIDS Response surface methodology
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