To provide and accumulate cytological data for bramble breeding in China, karyotypes of 8 Raspberry cultivars and 7 Blackberry cultivars introduced from foreign countries were investigated in the article. The results ...To provide and accumulate cytological data for bramble breeding in China, karyotypes of 8 Raspberry cultivars and 7 Blackberry cultivars introduced from foreign countries were investigated in the article. The results showed that all the 15 bramble cuitivars were of euploids and no aeuploids were found. All the 8 Raspberry cultivars were found to have 14 chromosomes, and the karyotype formulae of 2n=2x=14=14m, 2n=2x=14=14m (2SAT) and 2n=2x=14=14m (4SAT) were found respectively in 4 cultivars of ‘Algonquin’, ‘Reveille’, ‘Dinkum’ and ‘Ploana’, 3 cultivars of ‘Chilcotin’, ‘Nova’, and ‘Bristol’, and cultivar of ‘Chilliwack’. In the contrast, three various chromosome numbers of 28, 42 and 49 were found in the 7 Blackberry cultivars, their karyotypes formulae were as follows: 2n=4x=28=28m for ‘Arapaho’, ‘Shawnee’ and ‘Navaho’; 2n=6x=42=38m (6SAT) + 4sm for ‘Ollalie’; and 2n=7x=49=49m for ‘Black Butte’, ‘Boysen’ and ‘Kotata’. The karyotypes of all the bramble cultivars,except that of ‘Ollalie’,which was composed of metacentric chromosomes and submetacentric chromosomes and had a karyotype asymmetry type 2A, were composed of metacentric chromosome with type 1A asymmetry. Chromosome numbers of 9 bramble cultivars ‘Algonquin’, ‘Chilcotin’, ‘Reveille’, ‘Chilliwack’, ‘Nova’, ‘Ploana’, ‘Dinkum’, ‘Bristol’ and ‘Black Butte’ and karyotypes of 15 bramble cultivars were reported for the first time.展开更多
草莓转录组中特异表达基因密码子的使用偏好性分析能够为基因异源表达提供参考。为了探究红蓝光处理对草莓转录组特异表达基因密码子使用偏好性的影响,以四川农业大学果树分子生物学实验室前期红蓝光处理后的草莓转录组数据为原始实验数...草莓转录组中特异表达基因密码子的使用偏好性分析能够为基因异源表达提供参考。为了探究红蓝光处理对草莓转录组特异表达基因密码子使用偏好性的影响,以四川农业大学果树分子生物学实验室前期红蓝光处理后的草莓转录组数据为原始实验数据,以白光处理的草莓转录组数据为原始对照数据,利用perl语言及测序结果中的FPKM值(fragments per kb per million fragments)对转录组数据进行筛选,通过Mobyle在线程序对筛选得到的特异表达基因进行密码子偏好性分析。结果表明:蓝光处理后的特异表达基因有114条,大约是红光处理的2倍;从GC含量结合GC3s含量来看,红光和蓝光处理中的特异表达基因各自均有91%偏好使用以A/T结尾的密码子;最优密码子中,红光特异基因偏好以A/T结尾的最优密码子所占的比例比蓝光特异基因多2.84%;密码子使用频率比较结果发现,酵母表达系统更适合用于2种处理特异表达基因的异源表达实验,拟南芥更适合作为2种处理特异表达基因的转基因受体。展开更多
文摘To provide and accumulate cytological data for bramble breeding in China, karyotypes of 8 Raspberry cultivars and 7 Blackberry cultivars introduced from foreign countries were investigated in the article. The results showed that all the 15 bramble cuitivars were of euploids and no aeuploids were found. All the 8 Raspberry cultivars were found to have 14 chromosomes, and the karyotype formulae of 2n=2x=14=14m, 2n=2x=14=14m (2SAT) and 2n=2x=14=14m (4SAT) were found respectively in 4 cultivars of ‘Algonquin’, ‘Reveille’, ‘Dinkum’ and ‘Ploana’, 3 cultivars of ‘Chilcotin’, ‘Nova’, and ‘Bristol’, and cultivar of ‘Chilliwack’. In the contrast, three various chromosome numbers of 28, 42 and 49 were found in the 7 Blackberry cultivars, their karyotypes formulae were as follows: 2n=4x=28=28m for ‘Arapaho’, ‘Shawnee’ and ‘Navaho’; 2n=6x=42=38m (6SAT) + 4sm for ‘Ollalie’; and 2n=7x=49=49m for ‘Black Butte’, ‘Boysen’ and ‘Kotata’. The karyotypes of all the bramble cultivars,except that of ‘Ollalie’,which was composed of metacentric chromosomes and submetacentric chromosomes and had a karyotype asymmetry type 2A, were composed of metacentric chromosome with type 1A asymmetry. Chromosome numbers of 9 bramble cultivars ‘Algonquin’, ‘Chilcotin’, ‘Reveille’, ‘Chilliwack’, ‘Nova’, ‘Ploana’, ‘Dinkum’, ‘Bristol’ and ‘Black Butte’ and karyotypes of 15 bramble cultivars were reported for the first time.
文摘草莓转录组中特异表达基因密码子的使用偏好性分析能够为基因异源表达提供参考。为了探究红蓝光处理对草莓转录组特异表达基因密码子使用偏好性的影响,以四川农业大学果树分子生物学实验室前期红蓝光处理后的草莓转录组数据为原始实验数据,以白光处理的草莓转录组数据为原始对照数据,利用perl语言及测序结果中的FPKM值(fragments per kb per million fragments)对转录组数据进行筛选,通过Mobyle在线程序对筛选得到的特异表达基因进行密码子偏好性分析。结果表明:蓝光处理后的特异表达基因有114条,大约是红光处理的2倍;从GC含量结合GC3s含量来看,红光和蓝光处理中的特异表达基因各自均有91%偏好使用以A/T结尾的密码子;最优密码子中,红光特异基因偏好以A/T结尾的最优密码子所占的比例比蓝光特异基因多2.84%;密码子使用频率比较结果发现,酵母表达系统更适合用于2种处理特异表达基因的异源表达实验,拟南芥更适合作为2种处理特异表达基因的转基因受体。
