摘要
目的:建立高效液相指纹图谱,为白茅根药材及其颗粒的质量评价提供理论依据。方法:采用HPLC-PDA检测,色谱柱Diamonsil C18 (4.6 mm × 250 mm 5 μm),流速1.0 mL∙min−1,检测波长320 nm,流动相为乙腈-0.1%磷酸水,梯度洗脱,利用相似度评价软件和聚类分析对白茅根药材与配方颗粒样品进行分析。结果:以白茅根水溶性成分进行指纹图谱研究,白茅根药材与配方颗粒图谱同时导入相似度评价软件,10批白茅根药材的相似度均大于0.912,10批配方颗粒的相似度均大于0.944,药材与配方颗粒有18个共有峰,相似度在0.915~0.972范围内;聚类分析结果表明,白茅根药材和配方颗粒分别聚为两类。结论:建立的白茅根药材和配方颗粒的HPLC指纹图谱分析方法灵敏,稳定性好,重复性高,体现了白茅根药材和配方颗粒的整体化学成分特征,为白茅根药材和配方颗粒的质量评价提供依据。
Objective: To establish a high-performance liquid chromatography (HPLC) fingerprint to provide a theoretical basis for the quality evaluation of Imperata cylindrica medicinal material and its granules. Method: HPLC-PDA method was adopted. Chromatographic column Diamonsil C18 (4.6 mm × 250 mm 5 μm), flow rate of 1.0 mL·min−1, detection wavelength of 320 nm, mobile phase of acetonitrile and 0.1% phosphoric acid water, gradient elution, gradient elution. Then, similarity evaluation software and cluster analysis were used to analyze the Imperata cylindrica medicinal material and formula particle samples. Result: Fingerprint analysis was conducted on the water-solu- ble components of Imperata cylindrica and its granules, and the fingerprint of Imperata cylindrica and its granules were simultaneously imported into the similarity evaluation software. The similarity of 10 batches of Imperata cylindrica medicinal materials was greater than 0.912, and the similarity of 10 batches of granules was greater than 0.944, there are 18 common peaks between the medicinal herbs and the formula particles, with a similarity range of 0.915 to 0.972;the clustering analysis results indicated that the medicinal materials and formula particles of Imperata cylindrica were clustered into two categories, respectively. Conclusion: The established HPLC fingerprint analysis method for Imperata cylindrica medicinal herb and formula granules is sensitive, stable, and highly reproducible, reflecting the overall chemical composition characteristics of Imperata cylindrica medicinal herb and formula granules, providing a basis for the quality evaluation of Imperata cylindrica medicinal herb and formula granules.
出处
《中医学》
2023年第6期1397-1406,共10页
Traditional Chinese Medicine
