摘要
【目的】测定药用植物白花丹中重要植物次生物质白花丹素对草地贪夜蛾Spodoptera frugiperda 2龄幼虫的毒力,并明确亚致死浓度(LC 25浓度)白花丹素对草地贪夜蛾亲代(F_(0))和子一代(F_(1))生长发育及解毒酶活性的影响。【方法】白花丹素通过饲料混毒法处理草地贪夜蛾2龄幼虫(蜕皮后的第2天)测定其对草地贪夜蛾的毒力,同时以LC 25浓度(0.343 mg/g)白花丹素处理草地贪夜蛾2龄幼虫,统计记录F_(0)代与F_(1)代幼虫历期、化蛹率、蛹历期、雌雄成虫寿命和成虫单雌产卵量,构建年龄-龄期两性生命表。LC_(25)浓度白花丹素处理草地贪夜蛾2龄幼虫24 h后,测定羧酸酯酶(carboxylesterase,CarE)、谷胱甘肽S-转移酶(glutathione S-transferase,GST)和细胞色素P450单加氧酶(cytochrome P450 monooxygenase,CYP450)3种解毒酶的活性。【结果】白花丹素对草地贪夜蛾2龄幼虫7 d内的致死中浓度(median lethal concentration,LC_(50))和LC_(25)值分别为0.607和0.343 mg/g。与溶剂对照(0.1%丙酮)对比,LC_(25)浓度白花丹素处理草地贪夜蛾2龄幼虫后亲代(F_(0))的化蛹率显著下降了25.13%,雌雄蛹历期分别显著缩短了1.29和1.08 d,成虫的单雌产卵量显著下降了47%,产卵期和成虫寿命分别显著降低了1.75和1.19 d;子代(F_(1))的平均世代周期(mean generation time,T)显著缩短了0.91 d。LC_(25)浓度白花丹素处理草地贪夜蛾2龄幼虫24 h可显著诱导其体内CarE,GST和CYP4503种解毒酶活性,分别为溶剂对照的1.28,1.30和1.42倍。【结论】LC_(25)浓度白花丹素对亲代(F_(0))草地贪夜蛾的生长发育和繁殖产生明显的不利影响,且处理草地贪夜蛾2龄幼虫24 h可显著提高3种解毒酶CarE,GST和CYP450酶活性,该结论有助于将植物次生物质白花丹素作为防治草地贪夜蛾的潜在生物防治手段。
【Aim】To determine the toxicity of plumbagin(an important plant secondary substance from Chinese medicine Plumbago zeylanica)to the 2nd instar larvae of Spodoptera frugiperda and ascertain the sublethal effect of a sublethal concentration(LC_(25))of plumbagin on the growth,development and detoxifying enzyme activities of the F_(0) and F_(1) generations of S.frugiperda.【Methods】The toxicity of plumbagin against the 2nd instar larvae of S.frugiperda(the 2nd day after ecdysis)was determined using insecticide incorporated artificial diet bioassay.After the 2nd instar larvae were exposed to LC_(25)(0.343 mg/g)of plumbagin,the larval duration,pupation rate,pupal duration,female and male adult longevity,and number of eggs laid per female adult of the F_(0) and F_(1) generations of S.frugiperda were recorded,the age-stage,two-sex life table was constructed,and the activities of three detoxifying enzymes including carboxylesterase(CarE),glutathione S-transferase(GST)and cytochrome P450 monooxygenase(CYP450)were determined at 24 h after treatment.【Results】The median lethal concentration(LC_(50))and LC_(25) values of plumbagin against the 2nd instar larvae of S.frugiperda in 7 d were 0.607 and 0.343 mg/g,respectively.For the parental generation(F_(0))of S.frugiperda,LC_(25) of plumbagin significantly decreased the pupation rate by 25.13%,significantly shortened the female and male pupal duration by 1.29 and 1.08 d,respectively,significantly decreased the number of eggs laid per female by 47%,and significantly shortened the oviposition period and adult longevity by 1.75 and 1.19 d,respectively,compared to the vehicle control(0.1%acetone).For the offspring generation(F_(1))of S.frugiperda,LC_(25) of plumbagin only significantly shortened the mean generation time(T)by 0.91 d,compared to the vehicle control.LC_(25) of plumbagin significantly induced the activities of the three detoxifying enzymes CarE,GST and CYP450 in the 2nd instar larvae of S.frugiperda after treatment for 24 h,which were increased to 1.28-,1.30-and 1.42-fold as high as those in the vehicle control.【Conclusion】LC_(25) of plumbagin had obvious adverse effects on the growth,development and fecundity of the F_(0) generation of S.frugiperda,and significantly increased the activities of the three detoxifying enzymes CarE,GST and CYP450 in the 2nd instar larvae after treatment for 24 h,which will be helpful for using the plant secondary substance plumbagin as one of potent biocontrol strategies for this pest.
作者
闫泽同
孙晓雨
石旺鹏
黄欣蒸
甄丛爱
董民
YAN Ze-Tong;SUN Xiao-Yu;SHI Wang-Peng;HUANG Xin-Zheng;ZHEN Cong-Ai;DONG Min(Department of Entomology,College of Plant Protection,China Agricultural University,Beijing 100193,China;The Modern Agricultural Industrial Technology System Beijing Innovation Team,Beijing 100193,China)
出处
《昆虫学报》
2025年第8期1067-1076,共10页
Acta Entomologica Sinica
基金
现代农业产业技术体系北京市创新团队BAIC08-2025-YJ03
2024年度中国农业大学植物保护学院“一流本科专业建设计划”教改项目
中国农业大学大学生创新训练项目(X2023100190834)。
关键词
草地贪夜蛾
白花丹素
生命表
亚致死效应
解毒酶
Spodoptera frugiperda
plumbagin
life table
sublethal effect
detoxifying enzyme
作者简介
闫泽同,男,2005年1月生,山西临汾人,本科生,研究方向为害虫生物防治,E-mail:746794867@qq.com;通讯作者:石旺鹏,E-mail:wpshi@cau.edu.cn;通讯作者:甄丛爱,E-mail:zhencongai@126.com。
