摘要
为探索长鳍吻鮈幼鱼的肠排空特征,构建最佳数学模型,确定最适投喂频率,在水温19~20℃条件下,饱食投喂体质量(11.74±0.45) g的长鳍吻鮈幼鱼,于投喂后0、1、2、3、4、6、8、10、12 h,用间氨基苯甲酸乙酯甲磺酸盐麻醉,解剖3尾鱼观察肠内容物比例,饱食投喂后即刻作为肠排空程度0%时间点,于肠排空25%、50%、75%和99%时间点取肠组织,测定淀粉酶、糜蛋白酶和脂肪酶活性,研究肠排空过程中内容物质量和肠消化酶活性变化,比较线性模型、指数模型和平方根模型对肠内容物排空规律的曲线拟合效果。试验结果显示:长鳍吻鮈幼鱼肠内容物湿质量和干质量均随时间变化显著减少,即先缓慢降低,后迅速降低,再趋于平缓,平方根模型最适于模拟长鳍吻鮈幼鱼肠内容物湿质量和干质量排空过程。湿质量肠排空率为0.802 g/h, 80%肠排空时间为11.99 h;干质量肠排空率为0.801 g/h, 80%肠排空时间为11.38 h。肠排空过程中,肠道淀粉酶、糜蛋白酶和脂肪酶活性均先升后降。据此推算,长鳍吻鮈幼鱼最佳投喂时间间隔为12 h,投喂频率2次/d为宜。
To investigate the intestinal evacuation characteristics of juvenile Rhinogobio ventralis and to establish an optimal mathematical model for determining the most suitable feeding frequency,the propor tions of intestinal wet and dry contents were observed in the juvenile R.ventralis with body weight(11.74±0.45)g satiately fed and anaesthetize by MS-222 at 0,1,2,3,4,6,8,10 and 12 h after feeding at 19-20℃.The intestinal emptying time was estimated in R.ventralis at various emptying degrees of 0%(immediately satiated feeding),25%,50%,75%and 99%when the activities of amylase,chymotrypsin and lipase were measured to investigate the changes in content quality and intestinal digestive enzyme activity in the process of intestinal emptying,and the curve fitting effect of linear model,exponential model and square root model on the emptying of intestinal contents.It was found that the wet and dry weight of the intestinal contents of the juvenile was significantly decreased over time,with the initial slow reduction,followed by a rapid decrease,and then leveling off.The square root model was shown to be the most suitable model for simulating the wet and dry weight evacuation processes of the intestinal contents in juvenile R.ventralis,with the wet weight intestinal evacuation rate of 0.802 g/h,and 80%intestinal evacuation time at 11.99 hours,and with the dry weight intestinal evacuation rate of 0.801 g/h,and the 80%intestinal evacuation time at 11.38 hours.During the intestinal evacuation process,the activities of amylase,chymotrypsin,and lipase in the intestine were first increased and then decreased.Based on these findings,it is recommended that the optimal feeding interval for juvenile R.ventralis be 12 hours,with feeding frequency of twice a day.
作者
袁婷
黄耿
陈锋
俞伏虎
熊满堂
YUAN Ting;HUANG Geng;CHEN Feng;YU Fuhu;XIONG Mantang(Key Laboratory of Ecological Impacts of Hydraulic-Projects and Restoration of Aquatic Ecosystem,Ministry of Water Resources,Institute of Hydroecology,Ministry of Water Resources and Chinese Academy of Sciences,Wuhan 430079,China)
出处
《水产科学》
2025年第5期792-799,共8页
Fisheries Science
基金
武汉市2023年知识创新专项(2023020201020302).
关键词
长鳍吻鮈
肠内容物
肠排空模型
消化酶
Rhinogobio ventralis
intestinal content
intestinal evacuation rate model
digestive enzyme
作者简介
袁婷(1991-),女,助理研究员,研究方向:珍稀特有鱼类繁育.E-mail:yuanting@mail.ihe.ac.cn;通信作者:熊满堂(1993-),男,助理研究员,研究方向:水利水电工程水生态影响与减缓对策.E-mail:xiongmantang@mail.ihe.ac.cn.
